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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2021 06

Page:

941-944

Research Field:

Publishing date:

Info

Title:

Umbilical cord blood-derived DC enhance the role of NK and NKT cells killing leukemia cells

Author(s):

ZHAO Shuang; WU Song; YANG Shufen; ZHANG Qu

Shenyang Fourth People's Hospital,Liaoning Shenyang 110031,China.

Keywords:

leukemia; umbilical cord blood; dendritic cells; natural killer cells; natural killer T cells

PACS:

R733.7

DOI:

10.3969/j.issn.1672-4992.2021.06.007

Abstract:

Objective:To investigate the mechanism of the cytotoxicity of NK and NKT cells enhanced by DC from umbilical cord blood on leukemia cells.Methods£ºUmbilical cord blood mononuclear cells (PBMC) were extracted and cultured into DC,NK and NKT lymphocytes in vitro.The killing effects of NK,NKT,NK+DC and NKT+DC on target cells were detected by using human myeloid leukemia K-562 cells as target cells.The level of IFN-¦Ã in the co-culture supernatant was detected.Results£ºThe killing effect of NKT+DC on target cells was significantly enhanced compared with that of NKT group (P£¼0.05).The killing effect of NK+DC on target cells was also significantly stronger than that of the NK group (P£¼0.05).The expression level of IFN-¦Ã was the highest in the NKT+DC+target cell group compared with other groups,and reached the highest at 72 h (P£¼0.05).The expression of IFN-¦Ã in the NK+DC+target group was higher than that in the NK+target group (P£¼0.05).Conclusion£ºDC can effectively enhance the killing effect of NKT cells and NK cells on leukemia cells by stimulating NKT cells to produce a large amount of IFN-¦Ã.

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