|Table of Contents|

The expression and clinical significance of Th17 cell correlation factor in patients with non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2021 03
Page:
413-417
Research Field:
Publishing date:

Info

Title:
The expression and clinical significance of Th17 cell correlation factor in patients with non-small cell lung cancer
Author(s):
ZHU Yong; SHI Shunbin; ZHANG Lin; QIAN Taotao; WANG Hailong
Department of Thoracic Surgery，the 9th People's Hospital of Suzhou，Jiangsu Suzhou 215200，China.
Keywords:
NSCLC; Th17 cell; correlation factor; clinical significance
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2021.03.011
Abstract:
Objective：By detecting the expression of Th17 cells and related factors in NSCLC，the clinical significance of Th17 cells in NSCLC was discussed.Methods：41 NSCLC patients admitted to our hospital from February 2016 to February 2019 were selected as the NSCLC group，and 19 healthy people who underwent physical examination in our hospital during the same period were selected as the normal control group(NC group).Peripheral blood，cancerous tissues and adjacent tissues of patients with NSCLC and peripheral blood of peoples in NC group were collected.The level of Th17 cells in peripheral blood was detected by flow cytometry.The level of IL-17 in the homogenate supernatant of peripheral blood and lung tissues was detected by enzyme-linked immunosorbent assay.IL-17(IL-17+cells) in tissues was detected by immunohistochemical staining.The levels of Th17 and IL-17 in peripheral blood，cancerous tissues and para-cancerous tissues of NSCLC patients with different pathological types and pathological stages were compared.Results：The levels of Th17 cells and IL-17 in peripheral blood of patients in NSCLC group were higher than those in NC group，and the difference was statistically significant(P<0.05).Immunohistochemical tests showed that IL-17+ cells were positively expressed in cytoplasm with brown-brown color and tan color，and typical IL-17+ cells were significantly scattered in the tumor tissues and stained with brown color.The levels of Th17 and IL-17 in cancer tissues of NSCLC patients were higher than those in para-cancerous tissues，and the difference was statistically significant(P<0.05).Th17 and IL-17 levels in peripheral blood of patients with different pathological types of lung squamous cell carcinoma and lung adenocarcinoma，as well as in cancerous tissues and para-cancerous tissues，were not statistically significant(P>0.05).Ⅲ-Ⅳ NSCLC，focal cancer tissue，tissue adjacent to carcinoma patients peripheral blood Th17，IL-17 levels were higher than Ⅰ-Ⅱ period，and the difference was statistically significant(P<0.05).Conclusion：The expression of Th17 cells and its related factors in peripheral blood and cancer tissues of NSCLC patients was significantly upregaged and increased with the increase of tumor pathological stages，which may be related to the occurrence and development of NSCLC.

References:

[1]王亚龙，王永岗.多原发肺癌研究现状和展望［J］.中华肿瘤防治杂志，2019，26(09)：670-674. WANG YL，WANG YG.Current situation and prospect of multiple primary lung cancer［J］.Chinese Journal of Cancer Prevention and Treatment，2019，26(09)：670-674.
[2]LU Y，MA T，WANG L，et al.Advances in lymph node metastasis and lymph node dissection in early non-small cell lung cancer［J］.Chinese Journal of Lung Cancer，2019，22(8)：520-525.
[3] SHROFF GS,GROOT PMD,PAPADIMITRAKOPOULOU VA，et al.Targeted therapy and immunotherapy in the treatment of non-small cell lung cancer［J］.Radiologic Clinics of North America，2018，56(3)：485-495.
[4] WENJIE G，SIHAN L，XIAOLI Z，et al.The coexpression of multi-immune inhibitory receptors on T lymphocytes in primary non-small-cell lung cancer［J］.Drug Design Development & Therapy，2017，28(11)：3367-3376.
[5] ASADZADEH Z，MOHAMMADI H，SAFARZADEH E，et al.The paradox of Th17 cell functions in tumor immunity［J］.Cellular Immunology，2017，322(10)：15-25.
[6] FUCIKOVA J，BECHT E，IRIBARREN K，et al.Calreticulin expression in human non-small cell lung cancers correlates with increased accumulation of antitumor immune cells and favorable prognosis［J］.Cancer Res，2016，76(7)：1746-1756.
[7]叶波，赵珩.第八版国际肺癌TNM分期修订稿解读［J］.中国肺癌杂志，2016，19(06)：337-342. YE B，ZHAO H.Interpretation of the eighth international lung cancer TNM staging revision［J］.Chinese Journal of Lung cancer，2016，19(06)：337-342.
[8]王朋敏，王鑫，张晶，等.IL-23/Th17相关细胞因子在基底细胞癌中的表达水平及意义［J］.中国皮肤性病学杂志，2020，34(05)：530-533. WANG PM，WANG X，ZHANG J，et al.Expression level and significance of IL-23/Th17-related cytokines in basal cell carcinoma［J］.Chinese Journal of Dermatovenerology，2020，34(05)：530-533.
[9]PRITCHARD H，ANGELIDAKIS G，JIANG Y，et al.Impact of CD4+T-cell count on sustained virologic response to direct-acting antivirals in HIV-negative cancer patients with chronic hepatitis C［J］.Journal of Hepatology，2018，68(12)：S283-S284.
[10] 何玲，刘慧萍，张国民，等.Th17/Treg细胞与卵巢早衰的关系［J］.中华中医药学刊，2017，35(04)：897-899. HE L，LIU HP，ZHANG GM，et al.Relationship between Th17/Treg cells and premature ovarian failure［J］.Chinese Journal of Traditional Chinese Medicine，2017，35(04)：897-899.
[11] PURVI MEHROTRA, JASON A COLLETT, SUSAN J GUNST，et al.Th17 cells contribute to pulmonary fibrosis and inflammation during chronic kidney disease progression after acute ischemia［J］.Am J Physiol Regul Integr Comp Physiol，2018，314(2)：265-273.
[12]陈立，林文源.IL-17~+γδT细胞在相关疾病中的免疫作用［J］.中国免疫学杂志，2018，34(11)：1756-1761. CHEN L，LIN WY.Immunological effects of IL-17 ~+T cells in related diseases［J］.Chinese Journal of Immunology，2018，34(11)：1756-1761.
[13] CHANG SEON HEE.T helper 17(Th17) cells and interleukin-17(IL-17) in cancer［J］.Archives of Pharmacal Research，2019，42(7)：549-559.
[14]陈钢，段静静，苏文，等.非小细胞肺癌患者外周血Th17淋巴细胞和白细胞介素17的表达及其意义［J］.肿瘤研究与临床，2018，30(01)：29-33. CHEN G，DUAN JJ，SU W，et al.Expression of Th17 lymphocytes and interleukin-17 in peripheral blood of patients with non-small cell lung cancer and its significance［J］.Cancer Research and Clinic，2018，30(01)：29-33.
[15] HUANG Q，DUAN L，QIAN X，et al.IL-17 promotes angiogenic factors IL-6，IL-8，and VEGF production via Stat1 in lung adenocarcinoma［J］.Sci Rep，2016，7(6)：36551.
[16] 王晓琴.Th17细胞及Treg细胞在非小细胞肺癌的免疫调节作用［J］.中国肿瘤生物治疗杂志，2018，25(2)：187-191. WANG XQ.Immunomodulatory effects of Th17 and Treg cells in non-small cell lung cancer［J］.Chinese Journal of Cancer Biotherapy，2018，25(2)：187-191.
[17]马维江，王平，杨立民，等.辅助性T细胞17和调节性T细胞的免疫功能及其对非小细胞肺癌免疫调节作用的研究进展［J］.肿瘤研究与临床，2020，32(01)：66-69. MA WJ，WANG P，YANG LM，et al.Immune function of helper T cells 17 and regulatory T cells and its effect on immune regulation of non-small cell lung cancer［J］.Cancer Research and Clinical Practice，2020，32(01)：66-69.
[18] 耿婉如，高峨嵋，贺航咏.Th17细胞对肺癌双向调节作用的研究进展［J］.细胞与分子免疫学杂志，2017，33(4)：550-554. GENG WR，GAO EM，HE HY.Progress in the study of bidirectional regulation of lung cancer by Th17 cells［J］.Journal of Cell and Molecular Immunology，2017，33(4)：550-554.
[19]王守杰，王建莉，蔡志坚.Th17细胞及其在肿瘤免疫中作用的最新进展［J］.中国肿瘤生物治疗杂志，2018，25(03)：293-299. WANG SJ，WANG JL，CAI ZJ.Recent advances in Th17 cells and its role in tumor immunity［J］.Chinese Journal of Cancer Biotherapy，2018，25(03)：293-299.

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苏州市科学技术项目（编号：WS201313）
Last Update: 2020-12-31