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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2020 02

Page:

181-184

Research Field:

Publishing date:

Info

Title:

The effect and possible angiogenesis mechanism of AFP transfection of SMMC-7721 xenografts in nude mice

Author(s):

Wei Bai¹; Chen Jingsan¹; Yang Shengli²; Zhan Jing¹

1.Department of Oncology,Liyuan Hospital,the Affiliated Hospital of Tongji Medical University,Huazhong University of Science and Technology,Hubei Wuhan 430077,China;2.Cancer Center of Union Hospital,the Affiliated Hospital of Tongji Medical University,Huazhong University of Science and Technology,Hubei Wuhan 430022,China.

Keywords:

hepatocellular carcinoma; AFP; tumor growth; angiogenesis introduction

PACS:

R735.7

DOI:

10.3969/j.issn.1672-4992.2020.02.001

Abstract:

Objective:To investigate the effect and possible angiogenesis mechanism of AFP gene transfection of SMMC-7721 xenografts in nude mice.Methods:An expressing vector encoding AFP was recombined and then transfected into cultured SMMC-7721 with lipofectin.SMMC-7721/AFP and SMMC-7721 were inoculated nude mice to establish nude mice subcutaneous transplantation tumor of hepatocellular carcinoma model.Nude mice tumor volume and quality were observed dynamically HOXA7,eIF4E,VEGFA,FGF2 and MDM2 were measured in tumor tissue by real time RT-PCR and Western blot.Results:SMMC-7721/AFP and SMMC-7721/CON were established.Then xenografts in nude mice were successfully established.Compared with untransfected group,AFP gene transfection in experimental group promoted tumor volume and quality with significant difference.The volume and quality of the xenografts in nude mice were （307.71±47.63）mm3 and （20.243±0.411）g separately.The mRNA expression of HOXA7，eIF4E，VEGFA，FGF2 and MDM2 were upregulated.RT-PCR showed that AFP overexpression improved the mRNA expression of HOXA7,eIF4E and FGF2 mRNA to 2.488±1.155，23.828±2.465 and 4.407±1.164 with significance.However,VEGFA mRNA expression improved without significant difference.And Western blot results showed that HOXA7 was significantly increased in SMMC-7721/AFP compared to that in SMMC-7721/CON.However,the protein level of FGF2 and VEGFA had no significant alteration.Conclusion:AFP gene transfection can promote the growth of nude mouse transplantation tumor of hepatocellular carcinoma significant,which may be related with tumor angiogenesis.

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Memo

Memo:

湖北省自然科学基金（编号：2014CFB960）

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Last Update: 2019-11-29