|Table of Contents|

The mechanism of pristimerin inhibits proliferation of human glioblastoma U251 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2019 03
Page:
363-366
Research Field:
Publishing date:

Info

Title:
The mechanism of pristimerin inhibits proliferation of human glioblastoma U251 cells
Author(s):
Xu GaofengBao GangBai XiaobinQi LeiXie WanfuLi Ruichun
Department of Neurosurgery,the First Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710061,China.
Keywords:
pristimerinhuman glioblastomacell proliferationPI3K/AKT signalling
PACS:
R739.4
DOI:
10.3969/j.issn.1672-4992.2019.03.001
Abstract:
Objective:To explore the impact of pristimerin on cell proliferation of U251 cells.Methods:After treating with different concentrations of pristimerin(2,4,8 μmol/L) for 24 h,the viability of U251 cells was measured using MTT assay.Cell cycle was detected by cell cycle staining Kit.The expression levels of PCNA(proliferating cell nuclear antigen),p-PI3K,PI3K,p-AKT and AKT were determined by Western blot.Results:Compared with the control group,pristimerin markedly reduced the cell viability of U251 in a dose dependent way.Additionally,the expression levels of PCNA protein were obviously downregulated in pristimerin-treated U251 cells.We also found that the cell numbers in G1 period were obviously increased while the cell numbers in S period were markedly decreased in U251 cells treated by pristimerin.Eventually,the ratios of p-PI3K/PI3K and p-AKT/AKT were markedly reduced in pristimerin-treated U251 cells.Conclusion:Pristimerin can inhibit cell proliferation of human glioblastoma by inhibiting the activation of PI3K/AKT signalling.

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陕西省科技研究发展(攻关)计划项目(编号:2014K11-01-01-03)
Last Update: 2018-12-29