|Table of Contents|

Effects of arsenic trioxide on invasion and migration and USP22 expression in human HepG2 hepatocarcinoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 05
Page:
672-675
Research Field:
Publishing date:

Info

Title:
Effects of arsenic trioxide on invasion and migration and USP22 expression in human HepG2 hepatocarcinoma cells
Author(s):
Ni Boxiong1; Yang Yanmei2; Chen Xiaoning1; Sun Shibo1
1.Department of General Surgery，the Second Affiliated Hospital，Harbin Medical University，Heilongjiang Harbin 150086，China；2.Center for Endemic Disease Control，Chinese Center for Disease Control and Prevention，Harbin Medical University，Heilongjiang Harbin 150081，China.
Keywords:
As2O3; HepG2 cells; invasion and migration; USP22 protein
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2018.05.006
Abstract:
Objective：To investigate the effects of arsenic trioxide (As2O3) of various concentrations on invasion，migration and USP22 expression in human HepG2 hepatocarcinoma cells and the underlying molecular mechanism.Methods：CCK8 was used to test the proliferation levels of HepG2 cells treated with As2O3 of different concentrations in different time.Wound-healing and Transwell assays were used to examine the effects of As2O3 of different concentrations on cell migration and invasion.The protein levels of USP22 treated with As2O3 of different concentrations were determined by Western blot.Results：With the increase of As2O3 concentration and time，the proliferation of HepG2 cells was obviously inhibited.The migration，invasion and the expression of USP22 protein in HepG2 cells which were treated with 2，4，8 μmol/L As2O3 respectively for 24 h were obviously decreased compared with the control group.Conclusion：As2O3 could inhibit the migration and invasion of human hepatocellular carcinoma HepG2 cells，which may be related to the down-regulation of USP22 protein．

References:

［1］Siegel RL，Miller KD，Jemal A.Cancer statistics，2016［J］.CA Cancer J Clin，2016，66(1)：7-30.
［2］Wang ZY，Chen Z.Acute promyelocytic leukemia：from highly fatal to highly curable［J］.Blood，2008，111(5)：2505-2515.
［3］Thomas-Schoemann A，Batteux F，Mongaret C，et al.Arsenic trioxide exerts antitumor activity through regulatory T cell depletion mediated by oxidative stress in a murine model of colon cancer［J］.J Immunol，2012，189(11)：5171-5177.
［4］Wang L，Hu X，Xu Y，et al.Arsenic trioxide inhibits lung metastasis of mouse colon cancer via reducing the infiltration of regulatory T cells［J］.Tumour Biol，2016，37(11)：15165-15173.
［5］Chen Z，Zhang H，Yang L，et al.Construction of a metabolomics profile of arsenic trioxide effect in gastric carcinoma cell line SGC7901［J］.Acta Biochim Biophys Sin（Shanghai)，2016，48(5)：474-481.
［6］Xu Erdi，Yin Chunyan，Xiao Yanfeng，et al.Effects of As2O3 on gastric cancer cell strain SGC7901 growth and VEGF expression［J］.Modern Oncology，2013，21(11)：2419-2422.［徐尔迪，尹春燕，肖延风，等.三氧化二砷对胃癌细胞系SGC7901的生长及VEGF表达的影响［J］.现代肿瘤医学，2013，21(11)：2419-2422.］
［7］Zhang XY，Pfeiffer HK，Thorne AW，et al.USP22，an hSAGA subunit and potential cancer stem cell marker，reverses the polycomb-catalyzed ubiquitylation of histone H2A［J］.Cell Cycle，2008，7(11)：1522-1524.
［8］Tang B，Tang F，Li B，et al.High USP22 expression indicates poor prognosis in hepatocellular carcinoma［J］.Oncotarget，2015，6(14)：12654-12667.
［9］Yang X，Sun D，Tian Y，et al.Metformin sensitizes hepatocellular carcinoma to arsenic trioxide-induced apoptosis by downregulating Bcl2 expression［J］.Tumour Biol，2015，36(4)：2957-2964.
［10］Tang B，Liang X，Tang F，et al.Expression of USP22 and Survivin is an indicator of malignant behavior in hepatocellular carcinoma［J］.Int J Oncol，2015，47(6)：2208-2216.
［11］Ling SB，Sun DG，Tang B，et al.Knock-down of USP22 by small interfering RNA interference inhibits HepG2 cell proliferation and induces cell cycle arrest［J］.Cell Mol Biol（Noisy-le-grand)，2012，58 Suppl：OL1803-1808.
［12］Cui L，Gao B，Cao Z，et al.Downregulation of B7-H4 in the MHCC97-H hepatocellular carcinoma cell line by arsenic trioxide［J］.Mol Med Rep，2016，13(3)：2032-2038.
［13］Li HY，Cao LM.Inhibitory effect of arsenic trioxide on invasion in human hepatocellular carcinoma SMMC-7721 cells and its mechanism［J］.Chin J Cell Mol Imm，2012，28(12)：1254-1257.［李海燕，曹励民.三氧化二砷抑制人肝癌SMMC-772细胞侵袭及其机制［J］.细胞与分子免疫学杂志，2012，28(12)：1254-1257.］
［14］Chen JY，Chen YT，Lin ZY，et al.Inhibitory effect and mechanism of arsenic trioxide on human hepatocellular carcinoma cells migrationinduced by low dose of sorafenib［J］.Chin J Hepat Surg(Electronic Edition)，2016，5（2）：114-118.［陈洁盈，陈耀庭，林泽宇，等.三氧化二砷逆转低浓度索拉非尼对人肝癌细胞促迁移作用及其机制［J］.中华肝脏外科手术学电子杂志，2016，5（2）：114-118.］
［15］Ao N，Liu Y，Feng H，et al.Ubiquitin-specific peptidase USP22 negatively regulates the STAT signaling pathway by deubiquitinating SIRT1［J］.Cell Physiol Biochem，2014，33(6)：1863-1875.
［16］Ji M，Shi H，Xie Y，et al.Ubiquitin specific protease 22 promotes cell proliferation and tumor growth of epithelial ovarian cancer through synergy with transforming growth factor beta1［J］.Oncol Rep，2015，33(1)：133-140.
［17］Ning Z，Wang A，Liang J，et al.USP22 promotes the G1/S phase transition by upregulating FoxM1 expression via beta-catenin nuclear localization and is associated with poor prognosis in stage II pancreatic ductal adenocarcinoma［J］.Int J Oncol，2014，45(4)：1594-1608.

Memo

Memo:
-
Last Update: 1900-01-01