|Table of Contents|

MicroRNA-29a promotes epithelial-mesenchymal transition in non-small cell lung cancer through down-regulating PTEN gene expression

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 05
Page:
653-659
Research Field:
Publishing date:

Info

Title:
MicroRNA-29a promotes epithelial-mesenchymal transition in non-small cell lung cancer through down-regulating PTEN gene expression
Author(s):
Liang Yuan1; Feng Yangyang2; Li Linlin1; Shen Xiaoyu1; Xin Tian1; Zhao Yuwei1; Ma Rui1
1.Department of No.4 Internal Medicine，Cancer Hospital of China Medical University,Liaoning Cancer Hospital，Liaoning Shenyang 110042，China；2.Institute of Pathology and Pathophysiology，College of Basic Medical Science，China Medical University，Liaoning Shenyang 110122，China.
Keywords:
non-small cell lung cancer; microRNA-29a; PTEN; epithelial-mesenchymal transition
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2018.05.002
Abstract:
Objective：To explore the effects of microRNA-29a (miR-29a) and PTEN/Akt signaling pathway on TGF-β1-induced epithelial-mesenchymal transition (EMT) in non-small cell lung cancer (NSCLC).Methods：After 48 h of treatment with TGF-β1 (10 ng/ml)，the A549 cells were divided into the blank，negative control (NC)，miR-29a inhibitors (IN)，PTEN-siRNA and miR-29a inhibitors+PTEN-siRNA groups.EMT related factors and PTEN expressions were detected by qRT-PCR assay and Western blotting.The scratch test was conducted to observe cell migration.Xenograft tumor model in nude mice was used to evaluate the effects of miR-29a on EMT of NSCLC cells in vivo.Results：In NSCLC tissues，the expressions of miR-29a，Akt and p-Akt were up-regulated，while PTEN expression was down-regulated.Gene and protein expressions of E-cadherin and PTEN in the miR-29a inhibitors group were higher than the blank，NC，PTEN-siRNA and miR-29a inhibitors+PTEN-siRNA groups，while the expressions of N-cadherin，β-catenin，Vimentin and Slug were higher than other groups.miR-29a inhibitors significantly inhibit cell migration and invasion in NSCLC cell lines.In vivo xenograft experiment revealed that miR-29a inhibitor inhibits the growth of transplanted tumor through up-regulating E-cadherin and PTEN expressions and down-regulating the expressions of N-cadherin，β-catenin，Vimentin and Slug.Conclusion：These results suggest that miR-29a could promote TGF-β1-induced EMT in NSCLC cells through down-regulating PTEN expression.

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辽宁省自然科学基金项目（编号：20170540562）
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