|Table of Contents|

Over-expression of Lin28A induced by recombinant lentiviral vector fosters proliferation and invasion of gastric cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2018 02
Page:
163-165
Research Field:
Publishing date:

Info

Title:
Over-expression of Lin28A induced by recombinant lentiviral vector fosters proliferation and invasion of gastric cancer cells
Author(s):
Song Hu; Xu Yixin; Xu Teng; Fan Ruizhi; Cao Meng; Xu Wei; Song Jun
Department of Gastrointestinal Surgery,The Affiliated Hospital of Xuzhou Medical University,Jiangsu Xuzhou 221002,China.
Keywords:
Lin28; colon cancer; proliferation; invasion
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2018.02.001
Abstract:
Objective:To construct the recombinant lentiviral vector expressing Lin28A and to explore the effects of over-expression of Lin28A on proliferation and invasion of colon cancer cells.Methods:Human colon cancer HT-29 cells in vitro were stably transfected with lentivirus-mediated over-expression of Lin28A,and the expression levels of Lin28A，proliferating cell nuclear antigen (PCNA) and metalloproteinase-2 (MMP-2) were detected by using Western blot.The proliferative activity and metastatic capability of gastric cancer cells were assessed by methyl thiazol tetrazolium (MTT) and Transwell assays.Results:Over-expression of Lin28A could enhance proliferative activities and metastatic potential of colon cancer cells and up-regulate the expression of PCNA and MMP-2.Conclusion:Lin28A may function as an oncogene involved in colon cancer cell proliferation and invasion,and the mechanism may concern the expression of PCNA and MMP-2.

References:

［1］West JA,Viswanathan SR,Yabuuchi A,et al.A role for Lin28 in primordial germ-cell development and germ-cell malignancy［J］.Nature,2009,460(7257):909-913.
［2］King CE,Wang L,Winograd R,et al.Lin28B fosters colon cancer migration,invasion and transformation through let-7-dependent and -independent mechanisms［J］.Oncogene,2011,30(40):4185-4193.
［3］Pang M,Wu G,Hou X,et al.Lin28B promotes colon cancer migration and recurrence［J］.PLoS One,2014,9(10):e109169.
［4］Wang T,He Y,Zhu Y,et al.Comparison of the expression and function of Lin28A and Lin28B in colon cancer［J］.Oncotarget,2016,7(48):79605-79616.
［5］Treiber T,Treiber N,Plessmann U,et al.A compendium of RNA-binding proteins that regulate microRNA biogenesis［J］.Mol Cell,2017,66(2):270-284.
［6］Salim EI,Hegazi MM,Kang JS,et al.Expression patterns of cancer stem cell markers during specific celecoxib therapy in multistep rat colon carcinogenesis bioassays［J］.Asian Pac J Cancer Prev,2016,17(3):1023-1035.
［7］Ortega J,Li JY,Lee S,et al.Phosphorylation of PCNA by EGFR inhibits mismatch repair and promotes misincorporation during DNA synthesis［J］.Proc Natl Acad Sci USA,2015,112(18):5667-5672.
［8］Mao D,Qiao L,Lu H,et al.B-cell translocation gene 3 overexpression inhibits proliferation and invasion of colorectal cancer SW480 cells via Wnt/β-catenin signaling pathway［J］.Neoplasma,2016,63(5):705-716.
［9］Deng J,Chen W,Du Y,et al.Synergistic efficacy of Cullin1 and MMP-2 expressions in diagnosis and prognosis of colorectal cancer［J］.Cancer Biomark,2017,19(1):57-64.
［10］Schwegmann K,Bettenworth D,Hermann S,et al.Detection of early murine colorectal cancer by MMP-2/-9-Guided fluorescence endoscopy［J］.Inflamm Bowel Dis,2016,22(1):82-91.
［11］Liu YC,Kim SL,Park YR,et al.Parthenolide promotes apoptotic cell death and inhibits the migration and invasion of SW620 cells ［J］.Intest Res,2017,15(2):174-181.

Memo

Memo:
江苏省“六大人才高峰”资助项目（编号：2014-WSW-032）；江苏省“科教强卫工程”青年医学人才项目（编号：QNRC2016790）
Last Update: 2017-11-30