|Table of Contents|

Study of influence of S100A6 gene over-expression on growth of transplanted lung adenocarcinoma in nude rats

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 06
Page:
837-843
Research Field:
Publishing date:

Info

Title:
Study of influence of S100A6 gene over-expression on growth of transplanted lung adenocarcinoma in nude rats
Author(s):
Jiang Hua1; Nan Yandong1; Fang Yanfeng1; Jin Faguang1; Yang Shuanying2
1.Department of Respiratory and Critical Care Medicine,Tangdu Hospital,the Fourth Military Medical University,Shaanxi Xi'an 710038,China;2.Department of Respiratory Medicine，the Second Affiliated Hospital of Xi'an Jiaotong University,Shaanxi Xi'an 710004,China.
Keywords:
lung adenocarcinoma; S100A6; overexpression; apoptosis
PACS:
R73-35.4
DOI:
10.3969/j.issn.1672-4992.2017.06.001
Abstract:
Objective:To explore the influence of S100A6 gene overexpression on growth and apoptosis of transplanted lung adenocarcinoma in nude rats and its possible mechanism.Methods:18 healthy male Balb/c nude mice were randomly divided into three groups,empty vector control group(6 cases),negative control group(6 cases) and S100A6 gene overexpression group(6 cases).The transplanted lung adenocarcinoma models were established by subcutaneous injection of A549 lung adenocarcinoma cells line with empty vector,non-carrier vector and pLenO-DCE-S100A6,respectively.The volume,mass and pathology of transplanted tumor in different groups were observed.The S100A6 expression and cell apoptosis were detected by molecular biology methods.Results:The transplanted lung adenocarcinoma with S100A6 overexpression gene in nude mices was successfully established.The tumor volume and mass of S100A6 overexpression group were significantly higher than that of empty vector control group and negative control group(P<0.05).Pathological feature in the S100A6 overexpression group were that cells size became larger,nuclear pleomorphism was obvious and nucleocytoplasmic ratio was larger.There were more ribosome and rough endoplasmic reticulum in cytoplasm and some cancer thrombus appeared in the small blood vessels of alveolar septum.The expression of S100A6 among the three groups had significant difference(P＜0.05).Tumor cell apoptosis in S100A6 overexpression group were significantly higher than that in empty vector control group and negative control group(P＜0.05).Conclusion:The overexpression of S100A6 could significantly facilitate tumor cell growth via apoptosis pathway,which established experiment basis for explore molecular mechanism of lung cancer.

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Memo

Memo:
国家自然科学基金（编号：81001040）;第四军医大学优秀文职人员基金（编号：2011-01）;第四军医大学唐都医院后备人才基金（编号：5033）
Last Update: 2017-01-26