|Table of Contents|

Mechanism of ghrelin regulation of ERK signaling pathway on multidrug resistance of breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 05
Page:
687-690
Research Field:
Publishing date:

Info

Title:
Mechanism of ghrelin regulation of ERK signaling pathway on multidrug resistance of breast cancer cells
Author(s):
Qu Wenzhi; Li Zihao; Zhang Yunwei; Jin Guanghua; Tu Wei
Department of Breat Surgery，the Fourth Affiliated Hospital，China Medical University，Liaoning Shenyang 110032，China.
Keywords:
ghrelin; breast cancer; ERK; P-gp
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2017.05.004
Abstract:
Objective：To investigate the effect and mechanism of ghrelin regulated ERK signaling pathway on multidrug resistance of breast cancer cells.Methods：Human breast cancer cell MDA-MB-231 cell culture，to set up control group，doxorubicin group，ghrelin group，ghrelin combined with doxorubicin group，ghrelin combined with doxorubicin plus PD098059 inhibitor group，flow cytometry was used to detect cell apoptosis.The expression of ERK，p-ERK and P-gp protein was detected by Western-blot method.Results：Human breast cancer MDA-MB-231 cell apoptosis rate was lowest by ghrelin intervention(P<0.01)，and highest by doxorubicin intervention (P<0.01).Ghrelin combined with doxorubicin could inhibit the pro apoptotic effect of doxorubicin on human breast cancer MDA-MB-231 cells (P<0.01).Ghrelin combined with doxorubicin plus ERK signaling pathway specific blocker PD98059 could attenuate the inhibitory effect of ghrelin combined with doxorubicin on human breast cancer MDA-MB-231 cell apoptosis（P<0.01）.Compared with ghrelin group，the expression of ERK and the level of p-ERK were significantly decreased in doxorubicin group(P<0.05)，and P-gp protein expression of ghrelin group compared with the doxorubicin group was significantly increased(P<0.05).The expression of ERK and the level of p-ERK of ghrelin combined with doxorubicin group compared with doxorubicin group were significantly increased(P<0.05)，and the expression of P-gp protein was significantly increased(P<0.05).The expression of ERK and the level of p-ERK of ghrelin combined with doxorubicin plus inhibitor group compared with ghrelin combined with doxorubicin group were significantly decreased(P<0.01)，and there was no significant difference in the expression of P-gp protein between the two groups（P=0.07）.Conclusion：Certain concentration of ghrelin activates ERK signaling pathway in breast cancer cells to increase P-gp protein expression，inhibit apoptosis of breast cancer cells by doxorubicin and induce drug resistance.Blocking the ghrelin-ERK signaling pathway may reverse the occurrence of multidrug resistance in breast cancer cells.

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辽宁省科学技术计划项目（编号：2013225303）
Last Update: 2017-01-26