|Table of Contents|

The growth inhibitory effect of Trillium saponins on cervical cancer Hela cells and the possible mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 04
Page:
540-543
Research Field:
Publishing date:

Info

Title:
The growth inhibitory effect of Trillium saponins on cervical cancer Hela cells and the possible mechanism
Author(s):
Zeng Xiaocong1; Yuan Xinrong1; Xu Hui1; Sun Yang2; Huang Jianqing1; Yang Dongxiao1; Deng Shangfei1
1.Department of Oncology，No.422 Hospital of PLA，Guangdong Zhanjiang 524005,China；2.Department of Pharmacology，School of Pharmacy,the Fourth Military Medical University,Shaanxi Xi'an 710032,China.
Keywords:
Trillium saponins; proliferation; apoptosis; cervical cancer
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2017.04.009
Abstract:
Objective:To investigate the effects of Trillium saponins on the proliferation and the apoptosis of cervical cancer Hela cells.Methods:Hela cells were treated with different concentrations of Trillium saponins［(0~100.00)mg/L］.Cell proliferation was assessed by MTT assay and apoptosis was evaluated by flow cytometric analysis.The expressions of cleaved caspase-3,8,9,Bax and Bcl-2 of Hela cells were observed by Western blotting.Results:Trillium saponins could inhibit the growth of Hela significantly,and the IC50 value was （7.67±1.40）mg/L.Trillium saponins could induce the apoptosis of Hela cells.It upregulated the expression of cleaved caspase-3,9 and Bax and downregulated the expression of Bcl-2 in Hela cells concentration-dependently.Conclusion:Trillium saponins inhibited Hela proliferation,at least in part,inducing apoptosis of Hela through the mitochondrial pathway of apoptosis．

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Last Update: 2016-12-29