|Table of Contents|

The expression and function of lncRNA MEG3 in non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 03
Page:
398-401
Research Field:
Publishing date:

Info

Title:
The expression and function of lncRNA MEG3 in non-small cell lung cancer
Author(s):
Wei Rongfu
Department of Oncology,Gaochun County People's Hospital,Jiangsu Nanjing 211300,China.
Keywords:
NSCLClncRNAMEG3BCL-XLproliferation
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2017.03.016
Abstract:
Objective:To investigate the expression and its molecular mechanisms of lncRNA MEG3 in patients with non-small cell lung cancer (NSCLC).Methods:The expression levels of MEG3 in cancerous tissue and para-cancer normal lung tissue of NSCLC patients were detected by using qRT-PCR.The relationship between the expression of MEG3 and pathological characteristics were analyzed.After MEG3 transferred into A549 cells in vitro,the expression of MEG3 in A549 cells was detected by qRT-PCR and the cell viability was analyzed by MTT assay.Expression of BCL-2 and BCL-XL was detected by Western blot.Results:The expression of MEG3 was down-regulated in tissues of NSCLC compared with normal lung tissues which was associated with lymphatic metastasis,tumor size and TNM stages (P<0.05).The proliferation of A549 cells pcDNA3.0-MEG3-transfected was significantly inhibited compared with control.After transfection,the expression level of BCL-XL was obviously down-regulated (P<0.05).Conclusion:The expression of MEG3 was down-regulated in NSCLC.MEG3 might inhibit the proliferation of lung cancer cell partly through down-regulating the expression of BCL-XL.

References:

[1]Wang KC,HY Chang.Molecular mechanisms of long noncoding RNAs[J].Mol Cell,2011,43(6):904-914.
[2]Zhou X,Chen J,Tang W.The molecular mechanism of HOTAIR in tumorigenesis,metastasis,and drug resistance[J].Acta Biochim Biophys Sin (Shanghai),2014,46(12):1011-1015.
[3]Braconi C,Kogure T,Valeri N,et al.microRNA-29 can regulate expression of the long non-coding RNA gene MEG3 in hepatocellular cancer[J].Oncogene,2011,30(47):4750-4756.
[4]Zhou S,Wang J,Zhang Z.An emerging understanding of long noncoding RNAs in kidney cancer[J].J Cancer Res Clin Oncol,2014,140(12):1989-1995.
[5]Jia LF,Wei SB,Gan YH,et al.Expression,regulation and roles of miR-26a and MEG3 in tongue squamous cell carcinoma[J].Int J Cancer,2014,135(10):2282-2293.
[6]Yin DD,Liu ZJ,Zhang E,et al.Decreased expression of long noncoding RNA MEG3 affects cell proliferation and predicts a poor prognosis in patients with colorectal cancer[J].Tumour Biol,2015,13(2):123-128.
[7]Benetatos L,Hatzimichael E,Dasoula A,et al.CpG methylation analysis of the MEG3 and SNRPN imprinted genes in acute myeloid leukemia and myelodysplastic syndromes[J].Leuk Res,2010,34(2):148-153.
[8]Lu KH,Li W,Liu XH,et al.Long non-coding RNA MEG3 inhibits NSCLC cells proliferation and induces apoptosis by affecting p53 expression[J].BMC Cancer,2013,13(23):461-467.
[9]Sun M,Xia R,Jin F,et al.Downregulated long noncoding RNA MEG3 is associated with poor prognosis and promotes cell proliferation in gastric cancer[J].Tumour Biol,2014,35(2):1065-1073.
[10]Liu Z,Sun M,Lu K,et al.The long noncoding RNA HOTAIR contributes to cisplatin resistance of human lung adenocarcinoma cells via downregualtion of p21(WAF1/CIP1) expression[J].PLoS One,2013,8(10):e77293.
[11]Li P,Li J,Yang R,et al.Study on expression of lncRNA RGMB-AS1 and repulsive guidance molecule b in non-small cell lung cancer[J].Diagn Pathol,2015,10(23):63-69.
[12]Li J,Li P,Zhao W,et al.Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma[J].Cancer Cell Int,2015,15(34):48-52.
[13]Zhou Y,Zhang X,Klibanski A.MEG3 noncoding RNA:a tumor suppressor[J].J Mol Endocrinol,2012,48(3):R45-53.
[14]Qin R,Chen Z,Ding Y,et al.Long non-coding RNA MEG3 inhibits the proliferation of cervical carcinoma cells through the induction of cell cycle arrest and apoptosis[J].Neoplasma,2013,60(5):486-492.
[15]Wang P,Ren Z,Sun P.Overexpression of the long non-coding RNA MEG3 impairs in vitro glioma cell proliferation[J].J Cell Biochem,2012,113(6):1868-1874.
[16]Ying L,Huang Y,Chen H,et al.Downregulated MEG3 activates autophagy and increases cell proliferation in bladder cancer[J].Mol Biosyst,2013,9(3):407-411.
[17]Liu J,Wan L,Lu K,et al.The long noncoding RNA MEG3 contributes to cisplatin resistance of human lung adenocarcinoma[J].PLoS One,2015,10(5):e0114586.
[18]Xia Y,He Z,Liu B,et al.Downregulation of Meg3 enhances cisplatin resistance of lung cancer cells through activation of the WNT/β-catenin signaling pathway[J].Mol Med Rep,2015,12(3):4530-4537.

Memo

Memo:
-
Last Update: 2016-12-29