|Table of Contents|

Relationship between gene induced by transforming growth factor and tumor drug resistance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 03
Page:
358-361
Research Field:
Publishing date:

Info

Title:
Relationship between gene induced by transforming growth factor and tumor drug resistance
Author(s):
Zeng LiKe Changbin
Department of Pain Treatment,Taihe Hospital,Hubei Shiyan 442008,China.
Keywords:
transforming growth factorgene proteintumor drug resistance
PACS:
R730.5
DOI:
10.3969/j.issn.1672-4992.2017.03.006
Abstract:
Objective:To investigate the correlation of gene expression induced by transforming growth factor in tumor resistance.Methods:The expression of SKOV3 protein in human ovarian cancer cell line TGFBI was transfected with gene plasmid and empty plasmid.The expression of TGFBI protein was detected by Western blot,and the inhibition rate was detected by MTT assay.The effect of paclitaxel on apoptosis of two cells was detected by flow cytometry.Results:The expression of TGFBI protein was significantly higher than that of TGFBI protein after transfection of TGFBI gene.The inhibition rate of TGFBI was significantly higher than that of empty plasmid transfected cells (P<0.05) at the same concentration of paclitaxel and cisplatin.The apoptosis rate of TGFBI plasmid was (67.35±12.36)%,and the apoptosis rate of empty plasmid was (50.21±14.85)%.The apoptosis rate of TGFBI cells was significantly higher than that of empty plasmid transfected cells (P<0.05).Conclusion:High expression of TGFBI can reduce the drug resistance of ovarian cancer cell line SKOV3,improve the sensitivity of tumor cells to chemotherapeutic drugs,and can promote tumor cell apoptosis.

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Last Update: 2016-12-29