|Table of Contents|

Effect of silencing of ATM expression by siRNA on radiosensitivity of human gastric adenocarcinoma BGC823 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2017 02
Page:
190-194
Research Field:
Publishing date:

Info

Title:
Effect of silencing of ATM expression by siRNA on radiosensitivity of human gastric adenocarcinoma BGC823 cells
Author(s):
Zheng Xu; Liu Dan; Liu Fan
Department of Radiation Oncology,the Fourth Affiliated Hospital of China Medical University,Liaoning Shenyang 110032,China.
Keywords:
gastric cancer; ATM; siRNA; radiation sensitivity
PACS:
R735.2
DOI:
10.3969/j.issn.1672-4992.2017.02.007
Abstract:
Objective:To study the effect of interference of ataxia-telangiectasia mutated(ATM) expression of the radiosensitivity on human gastric cancer BGC823 cells.Methods:Using the plasmid of interfering RNA to transfect BGC823 cells．The mRNA and protein expression level of ATM was measured by RT-PCR,Real-time PCR and Western blot，respectively．Using the colonies formation assay to observe the change of cell radiosensitivity by different doses of irradiation．Cell cycle and cell apoptosis were analyzed by flow cytometry.Results:The expression of ATM in the BGC823 cells were knocked out by small interfering RNA(siRNA),the colonies formation numbers/ratios of BGC823-Ri-ATM cells were (15.4±0.9)%,(9.2±0.3)%,(2.2±0.1)% and (1.0±0.1)% by the different doses of irradiation (2,4,6,and 8Gy),which were lower than the BGC823-Vector's colonies formation numbers/ratios (28.1±0.6)%,(15.6±0.8)%,(5.3±0.1)% and (1.8±0.1)% respectively(P＜0.05).The flow cytometry detection that the BGC823-Ri-ATM cells were induced cell cycle arrest and apoptosis by 4Gy intensity radioactivity after 12h,but not incurred in the vector cells.Conclusion:ATM gene expression can affect the radiation sensitivity of gastric cancer cells,the silencing of ATM increase the radiotherapy sensitization of BGC823 cells.ATM gene may play an important role in gastric cancer radiation sensitivity.

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