|Table of Contents|

Expression and clinical significance of LINC01006 in esophageal cancer tissue

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 05
Page:
764-771
Research Field:
Publishing date:

Info

Title:
Expression and clinical significance of LINC01006 in esophageal cancer tissue
Author(s):
FANG Zhenghua1; ZHU Chen1; DU Mingyu2; XIE Peng1
1.Anqing Second People's Hospital,Anhui Anqing 246000,China;2.Jiangsu Cancer Hospital,Jiangsu Nanjing 210000,China.
Keywords:
LINC01006; esophageal squamous cell carcinoma; invasion; miR-449c
PACS:
R735.1
DOI:
10.3969/j.issn.1672-4992.2025.05.007
Abstract:
Objective:To investigate the expression,clinical significance of lncRNA LINC01006 in esophageal squamous cell carcinoma (ESCC),and to explore its possible mechanism of action.Methods:The tumors and corresponding adjacent tissues were collected from 60 patients with ESCC treated in our hospital from January 2016 to July 2019.RT-qPCR was used to detect LINC01006 expression in tissue.The relationship between LINC01006 expression and clinical features,overall survival (OS) of patients was analyzed.The effect of silencing LINC01006 on the proliferation,invasion of esophageal cancer cells was explored by CCK8 and Transwell experiments.Dual-luciferase reporter assay was employed to identify the relationship between miR-449c and LINC01006.Results:The results of RT-qPCR assay showed that LINC01006 expression in ESCC tissue was significantly higher than the corresponding adjacent tissues(5.755±2.602 vs 1.406±0.077,P＜0.01).And its expression level was closely related to the TNM stage of patients by Chi-quare test (P＜0.01).The results of Kaplan-Meier analysis indicated that the OS of patients with high expression of LINC01006 was significantly shorter than those with low expression(P＜0.01).Cox regression univariate and multivariate analysis showed that the expression of LINC01006 was an independent risk factor for OS (HR=4.245,95%CI:1.589~11.338,P=0.004;HR=3.356,95%CI:1.185~9.509,P=0.023) in patients with ESCC.Cell biological experiments exhibited that LINC01006 knockdown significantly reduced the proliferation,invasion of ESCC cells.Dual-luciferase reporter assay showed that miR-449c directly targeted LINC01006.The results of rescue experiments indicated that inhibition of miR-449c expression reversed the inhibition of tumor cell activity caused by silencing LINC01006.Western blot experimental result further validated that LINC01006 can activate the expression of MET protein by competitive binding to miR-449c.Conclusion:LINC01006 is significantly overexpressed in ESCC tissue and cells.Knocking down LINC01006 can inhibit the proliferation and invasion abilities of tumor cells via miR-449c-MET axis,which is able to be a new treatment target of ESCC.

References:

［1］ CAO W,QIN K,LI F,et al.Comparative study of cancer profiles between 2020 and 2022 using global cancer statistics (GLOBOCAN) ［J］.J Natl Cancer Cent,2024,4(2):128-134.
[2] LIU M,YANG W,GUO C,et al.Effectiveness of endoscopic screening on esophageal cancer incidence and mortality: A 9-year report of the endoscopic screening for esophageal cancer in China (ESECC) randomized trial ［J］.J Clin Oncol,2024,42(14):1655-1664.
[3] JU W,ZHENG R,ZHANG S,et al.Cancer statistics in Chinese older people,2022: current burden,time trends,and comparisons with the US,Japan,and the Republic of Korea ［J］.Sci China Life Sci,2023,66(5):1079-1091.
[4] ZHENG Y,LIANG G,YUAN D,et al.Perioperative toripalimab plus neoadjuvant chemotherapy might improve outcomes in resectable esophageal cancer:an interim analysis of a phase III randomized clinical trial ［J］.Cancer Commun (Lond),2024,44(10):1214-1227.
[5] GUO JC,HUANG TC,KUO HY,et al.Adjuvant chemoradiotherapy plus pembrolizumab for locally advanced esophageal squamous cell carcinoma with high risk of recurrence following neoadjuvant chemoradiotherapy:a single-arm phase II study ［J］.Cancer Immunol Immunother,2024,73(11):230.
[6] SUN Z,ZHAN L,WANG S,et al.Knockdown of long non-coding RNA LINC01006 represses the development of hepatocellular carcinoma by modulating the miR-194-5p/CADM1 axis ［J］.Ann Hepatol,2022,27 Suppl 1:100571.
[7] MA E,WANG Q,LI J,et al.LINC01006 facilitates cell proliferation,migration and invasion in prostate cancer through targeting miR-34a-5p to up-regulate DAAM1 ［J］.Cancer Cell Int,2020,20:515.
[8] CHEN M,ZOU C,TIAN Y,et al.An integrated ceRNA network identifies miR-375 as an upregulated miRNA playing a tumor suppressive role in aggressive prostate cancer ［J］.Oncogene,2024,43(21):1594-1607.
[9] WU Z,WANG H,FANG S,et al.MiR-449c inhibits gastric carcinoma growth ［J］.Life Sci,2015,137:14-19.
[10] MA F,LI Y,XIANG C,et al.Proteomic characterization of esophageal squamous cell carcinoma response to immunotherapy reveals potential therapeutic strategy and predictive biomarkers ［J］.J Hematol Oncol,2024,17(1):11.
[11] COAN M,HAEFLIGER S,OUNZAIN S,et al.Targeting and engineering long non-coding RNAs for cancer therapy ［J］.Nat Rev Genet,2024,25(8):578-595.
[12] HERMAN AB,TSITSIPATIS D,GOROSPE M.Integrated lncRNA function upon genomic and epigenomic regulation ［J］.Mol Cell,2022,82(12):2252-2266.
[13] LIANG Y,CHEN X,WU Y,et al.LncRNA CASC9 promotes esophageal squamous cell carcinoma metastasis through upregulating LAMC2 expression by interacting with the CREB-binding protein ［J］.Cell Death Differ,2018,25(11):1980-1995.
[14] LIU Y,LI C,FANG L,et al.Lipid metabolism-related lncRNA SLC25A21-AS1 promotes the progression of oesophageal squamous cell carcinoma by regulating the NPM1/c-Myc axis and SLC25A21 expression ［J］.Clin Transl Med,2022,12(6):e944.
[15] HUANG X,LIU C,LI H,et al.Hypoxia-responsive lncRNA G077640 promotes ESCC tumorigenesis via the H2AX-HIF1α-glycolysis axis ［J］.Carcinogenesis,2023,44(5):383-393.
[16] MA H,WENG F,TONG X,et al.LncRNA TRPM2-AS promotes endometrial carcinoma progression and angiogenesis via targeting miR-497-5p/SPP1 axis ［J］.Cell Mol Biol Lett,2024,29(1):93.
[17] TIAN L,HAN F,YANG J,et al.Long non-coding RNA LINC01006 exhibits oncogenic properties in cervical cancer by functioning as a molecular sponge for microRNA-28-5p and increasing PAK2 expression ［J］.Int J Mol Med,2021,47(4):46.
[18] SONG Y,WANG S,CHEN X.LINC01006 regulates the proliferation,migration and invasion of hepatocellular carcinoma cells through regulating miR-433-3p/CBX3 axis ［J］.Ann Hepatol,2021,25:100343.
[19] CHEN X,WANG A,YUE X.miR-449c inhibits migration and invasion of gastric cancer cells by targeting PFKFB3［J］.Oncol Lett,2018,16(1):417-424.
[20] ZHANG D,PAN N,JIANG C,et al.LncRNA SNHG8 sponges miR-449c-5p and regulates the SIRT1/FoxO1 pathway to affect microglia activation and blood-brain barrier permeability in ischemic stroke ［J］.J Leukoc Biol,2022,111(5):953-966.
[21] LI Q,LI H,ZHAO X,et al.DNA methylation mediated downregulation of miR-449c controls osteosarcoma cell cycle progression by directly targeting oncogene c-Myc ［J］.Int J Biol Sci,2017,13(8):1038-1050.
[22] SANDBOTHE M,BUURMAN R,REICH N,et al.The microRNA-449 family inhibits TGF-β-mediated liver cancer cell migration by targeting SOX4 ［J］.J Hepatol,2017,66(5):1012-1021.
[23] ZHANG L,WANG Y,ZHANG L,et al.LINC01006 promotes cell proliferation and metastasis in pancreatic cancer via miR-2682-5p/HOXB8 axis ［J］.Cancer Cell Int,2019,19:320.
[24] ZHANG Y,LIU H,ZHANG Q,et al.Long noncoding RNA LINC01006 facilitates cell proliferation,migration,and epithelial-mesenchymal transition in lung adenocarcinoma via targeting the microRNA 129-2-3p/CTNNB1 axis and activating Wnt/β-Catenin signaling pathway ［J］.Mol Cell Biol,2021,41(6):e0038020.
[25] WU Y,YU B,LI Y,et al.LINC01006 and miR-3199 serve as novel markers of poor prognosis in colon cancer and regulate cell proliferation,migration and invasion ［J］.Int J Gen Med,2022,15:1677-1687.

Memo

Memo:
National Natural Science Foundation of China(No.82002870）；国家自然科学基金（编号：82002870）
Last Update: 1900-01-01