|Table of Contents|

PAK5 and p53 competitively combine with DDX5 to promote the proliferation of breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 05
Page:
749-755
Research Field:
Publishing date:

Info

Title:
PAK5 and p53 competitively combine with DDX5 to promote the proliferation of breast cancer cells
Author(s):
CAI Yihang; WANG Leyan; ZHAO Xin; LI Yang
Molecular Cell Biology,College of Life Sciences,China Medical University,Liaoning Shenyang 110122,China．
Keywords:
phosphorylation; competitive combination; p21-activated kinase 5 (PAK5); DEAD-box helicase 5 (DDX5)
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2025.05.005
Abstract:
Objective:To explore the effects and possible mechanisms of phosphorylation modification of p21-activated kinase 5 (PAK5) on the binding of DDX5 to p53.Methods:In order to explore the effect of PAK5 on the binding of DDX5 and p53,in breast cancer cells overexpressing PAK5,Co-IP was used to verify the change of the binding ability of DDX5 and p53,and GST pulldown experiment was used to verify the effect of PAK5 on the direct binding ability of DDX5 and p53.Construct a DDX5 truncated plasmid and validate the binding domains of PAK5 and p53 with DDX5 through GST pulldown experiment.Construct a DDX5 phosphorylation site mutant plasmid,purify DDX5 proteins with different phosphorylation states,and validate the binding status of DDX5 and p53 through GST pulldown experiment.The Real-Time PCR experiment confirmed that PAK5 inhibits the expression of mature miR-143 and miR-145.Finally,CCK8 experiment was used to detect the effect of overexpression of DDX5 with different phosphorylation states on the proliferation of breast cancer cells.Results:Overexpression of PAK5 weakened the binding of DDX5 to p53,and promoted the proliferation of breast cancer cells.Conclusion:PAK5 may promote the growth of breast tumors by phosphorylating DDX5 and competitively binding to DDX5 with p53,and thereby inhibiting the expression of mature miR-143 and miR-145.

References:

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Memo

Memo:
辽宁省教育厅青年科学家项目（编号：LJ212410159117）;辽宁省沈阳市科技局2023年助力中国医科大学高质量发展专项（编号:23-506-3-01-34）
Last Update: 1900-01-01