|Table of Contents|

Metformin inhibits metabolism of multiple myeloma cells through hexokinase 2

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 05
Page:
743-748
Research Field:
Publishing date:

Info

Title:
Metformin inhibits metabolism of multiple myeloma cells through hexokinase 2
Author(s):
ZHANG Yuan1; 2; YAN Zhimin3; ZI Fuming1
1.Department of Hematology,the Second Affiliated Hospital of Nanchang University,Jiangxi Nanchang 330000,China;2.Department of Internal Medicine,Fuzhou Medical College of Nanchang University,Jiangxi Fuzhou 344000,China;3.Department of Hematology,the First Affiliated Hospital of Gannan Medical University,Jiangxi Ganzhou 341000,China.
Keywords:
metformin; multiple myeloma; HK2; glycolysis
PACS:
R733.3
DOI:
10.3969/j.issn.1672-4992.2025.05.004
Abstract:
Objective:To investigate the effect of metformin on glycolysis of multiple myeloma (MM) cells and its molecular mechanism.Methods:Human multiple myeloma cell lines ARP-1 and CAG were treated with different concentrations of metformin.The expression levels of HK2 and c-MYC were detected by Western blot.The glucose uptake rate and lactate levels inside and outside the cells were measured by colorimetry.The intracellular adenosine triphosphate (ATP) level was detected by CellTiter-LumiTM luminescence,and intracellular reduced nicotinamide adenine dinucleotide phosphate (NADPH) content was detected by ELISA method.Furthermore,the effect of metformin on glycolysis of ARP-1 cells was observed after knockout HK2 of ARP-1 cells by siRNA.Results:Metformin not only decreased the level of c-MYC,but also down-regulated the expression of HK2 and inhibited the glycolysis of MM cells.Knockdown of HK2 also inhibited glycolysis of MM cells,but promoted the expression of c-MYC,and the expression of c-MYC decreased after the use of metformin.Conclusion:Metformin inhibits glycolysis of multiple myeloma cell lines.HK2 may be a target for metabolic therapy of multiple myeloma.

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Memo:
江西省自然科学基金面上项目(编号：20202BABL206097)
Last Update: 1900-01-01