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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2025 04

Page:

576-582

Research Field:

Publishing date:

Info

Title:

miR-29a-3p inhibits cisplatin resistance of osteosarcoma cells by targeting TRAF5

Author(s):

KUANG Jian¹; DONG Wengang²; DING Jiayun¹; XIE Qing¹; LIU Wenyi¹; ZHOU Zhijian¹; LIU Jun¹

1.Center for Diseases Control and Prevention,Southern Theater Command,Guangdong Guangzhou 510603,China;2.Department of Emergency Surgery,Shaanxi Provincial People¡¯s Hospital,Shaanxi Xi¡¯an 710068,China.

Keywords:

osteosarcoma; miR-29a-3p; TRAF5; cisplatin resistance

PACS:

R738.1

DOI:

10.3969/j.issn.1672-4992.2025.04.005

Abstract:

Objective:To explore the effect of miR-29a-3p on cisplatin resistance of osteosarcoma cells by regulating the expression of tumor necrosis factor receptor associated factor-5 (TRAF5).Methods:Real-time PCR assay was used to detect the level of miR-29a-3p in osteosarcoma cell lines,then miR-29a-3p level in 143B cells was detected by real-time PCR assay after administration of cisplatin to osteosarcoma 143B cells to inspect that whether miR-29a-3p changed along the administration time and concentration or not.After transfection of miR-29a-3p mimics into cisplatin-administered 143B cells,flow cytometry and Western blotting assays were carried out to investigate the effects of miR-29a-3p on apoptosis of 143B cells,and CCK-8 assay was carried out to study the effects of miR-29a-3p on proliferation of 143B cells.Online bioinformatics prediction through TargetScan website and dual luciferase reporter assays were performed to testify the targeting role of miR-29a-3p on TRAF5.Real-time PCR and Western blotting detected the expression level of miR-29a-3p and TRAF5.Results:Real-time PCR results showed that miR-29a-3p expression was aberrantly downregulated in osteosarcoma cell lines compared with osteoblast cell line hFOB1.19,and was the lowest in 143B cells.After administration of cisplatin,miR-29a-3p level was gradually downregulated dependent on increment of cisplatin concentration and action time in osteosarcoma 143B cells.After transfection of miR-29a-3p mimics into cisplatin-administered 143B cells,flow cytometry result demonstrated that miR-29a-3p improved cisplatin-administered 143B cells apoptosis,and CCK-8 assays results demonstrated that miR-29a-3p inhibited cisplatin-administered 143B cells proliferation.Western blotting result showed that the upgrading of miR-29a-3p significantly changed the expression of apoptosis-related genes of 143B cells after cisplatin treatment.Bioinformatics predicts that TRAF5 was the target gene of miR-29a-3p.Dual luciferase reporter assay confirmed that miR-29a-3p can target the TRAF5 inhibition.After over-expression of miR-29a-3p,the expression level of TRAF5 in 143B cells was lowered.Conclusion:miR-29a-3p may inhibit the cisplatin resistance of osteosarcoma cells 143B by inhibiting the expression of TRAF5.

References:

£Û1£Ý KEVIN M MCMAHON,VINCENT P EATON,GREGORY J CICHON,et al.Utilization of palliative care in osteosarcoma:A national cancer database review £ÛJ£Ý.Am J Hosp Palliat Care,2023,40(8):926-935.
£Û2£Ý SINCHUN HWANG,MEERA HAMEED,MARK KRANSDORF.The 2020 World Health Organization classification of bone tumors:what radiologists should know £ÛJ£Ý.Skeletal Radiol,2023,52(3):329-348.
£Û3£Ý YAN P,WANG J,YUE B,et al.Unraveling molecular aberrations and pioneering therapeutic strategies in osteosarcoma £ÛJ£Ý.Biochim Biophys Acta Rev Cancer,2024,1879(5):189171.
£Û4£Ý BERLANGA P,MU¦rOZ L,PIQUERAS M,et al.miR-200c and phospho-AKT as prognostic factors and mediators of osteosarcoma progression and lung metastasis £ÛJ£Ý.Mol Oncol,2016,10(7):1043-1053.
£Û5£Ý REN SQ,PAN RF,WANG ZD.Development and experimental verification of novel angiogenesis related prognostic model and immune infiltration characterization in osteosarcoma £ÛJ£Ý.Discov Oncol,2024,15(1):411.
£Û6£Ý HE HT,NI JD,HUANG J.Molecular mechanisms of chemoresistance in osteosarcoma (review)£ÛJ£Ý.Oncol Lett,2014,7(5):1352-1362.
£Û7£Ý WANG Y,HU Q,CAO Y,et al.FOSL1 promotes stem cell like characteristics and anoikis resistance to facilitate tumorigenesis and metastasis in osteosarcoma by targeting SOX2£ÛJ£Ý.Int J Mol Med,2024,54(5):94.
£Û8£Ý TONI NUNES,DIADDIN HAMDAN,CHRISTOPHE LEBOEUF,et al.Targeting cancer stem cells to overcome chemoresistance £ÛJ£Ý.Int J Mol Sci,2018,19(12):4036.
£Û9£Ý SUSANNA WEIDLINGER,SATU GRABER,IRINA BRATSCHI,et al.A systematic review of the gonadotoxicity of osteosarcoma and Ewing¡¯s sarcoma chemotherapies in postpubertal females and males £ÛJ£Ý.J Adolesc Young Adult Oncol,2024,13(4):597-606.
£Û10£Ý PAN GT,LIU YH,SHANG LR,et al.EMT-associated microRNAs and their roles in cancer stemness and drug resistance £ÛJ£Ý.Cancer Commun (Lond),2021,41(3):199-217.
£Û11£Ý WANG JY,YANG Y,MA YJ,et al.Potential regulatory role of lncRNA- miRNA-mRNA axis in osteosarcoma £ÛJ£Ý.Biomed Pharmacother,2020,121:109627.
£Û12£Ý YANG ZY,ZHAO N,CUI J,et al.Exosomes derived from cancer stem cells of gemcitabine-resistant pancreatic cancer cells enhance drug resistance by delivering miR-210£ÛJ£Ý.Cell Oncol (Dordr),2020,43(1):123-136.
£Û13£Ý SAMIRA ABEDI,ALI BEHMANESH,FARID NAJD MAZHAR,et al.Machine learning and experimental analyses identified miRNA expression models associated with metastatic osteosarcoma £ÛJ£Ý.Biochim Biophys Acta Mol Basis Dis,2024,1870(7):167357.
£Û14£Ý LI C,YU X,GUO D,et al.Association between common polymorphisms in ERCC gene and prognosis of osteosarcoma in patients treated with chemotherapy:a meta-analysis £ÛJ£Ý.Onco Targets Ther,2018,11:3495-3504.
£Û15£Ý SAVAGE SA,MIRABELLO L.Bone cancer:is the osteosarcoma genome targetable £ÛJ£Ý.Nat Rev Endocrinol,2017,13(9):506-508.
£Û16£Ý KANSARA M,THOMSON K,PANG P,et al.Infiltrating myeloid cells drive osteosarcoma progression via regulation of IL23£ÛJ£Ý.Cancer Discov,2019,9(11):1511-1519.
£Û17£Ý MARIL¦_ FANELLI,ELISA TAVANTI,MARIA PIA PATRIZIO,et al.Cisplatin resistance in osteosarcoma:In vitro validation of candidate DNA repair-related therapeutic targets and drugs for tailored treatments £ÛJ£Ý.Front Oncol,2020,10:331.
£Û18£Ý XI XH,BAO YZ,ZHOU YF,et al.Oncogenic gene TRIM10 confers resistance to cisplatin in osteosarcoma cells and activates the NF-¦ÊB signaling pathway £ÛJ£Ý.Cell Biol Int,2021,45(1):74-82.
£Û19£Ý QIU CS,SU WL,SHEN NN,et al.MNAT1 promotes proliferation and the chemo-resistance of osteosarcoma cell to cisplatin through regulating PI3K/Akt/mTOR pathway£ÛJ£Ý.BMC Cancer,2020,20(1):1187.
£Û20£Ý WANG CR,ZHANG YH,KONG WH,et al.Delivery of miRNAs using nanoparticles for the treatment of osteosarcoma £ÛJ£Ý.Int J Nanomedicine,2024,22(19):8641-8660.
£Û21£Ý YAN CL,DOU YN,XIA RL,et al.Research progress on the role of lncRNA,circular RNA,and microRNA networks in regulating ferroptosis in osteosarcoma £ÛJ£Ý.Biomed Pharmacother,2024,176:116924.
£Û22£Ý ¿ï½¡, ¶­ÎĸÕ, ¶¡¼ÒÔÆ, µÈ. miR-29a-3pÒÖÖƹÇÈâÁöϸ°û¸ÉÐÔ¼°Æä·Ö×Ó»úÖÆ£ÛJ/OL£Ý. ÏÖ´úÖ×Áöҽѧ, 2025£Û2025-02-12£Ý.https://kns.cnki.net/kcms2/article/abstract?v=YBmesx2FU7mivw3Mf9IRNNM8fSlBtwDlJjFqjk0vYqC2Zu4cVl3Xp2-9BVFFKi51oytHzUZqX1ybY--0Nyiizl7EHzFAZJtnt7hyerSsIvt-DaHMBYsGxtzu6pFekktCLL1pzc5yYO0DMxz4qh9ERg4fBXhdpnTiWf2z6re99UogLQvCLgvwOu0_9nkZZNeH&uniplatform=NZKPT&language=CHS.KUANG J,DONG WG,DING JY,et al.miR-29a-3p inhibits osteosarcoma cells stemness and its molecular mechanism£ÛJ/OL£Ý.Modern Oncology,2025£Û2025-02-12£Ý.https://kns.cnki.net/kcms2/article/abstract?v=YBmesx2FU7mivw3Mf9IRNNM8fSlBtwDlJjFqjk0vYqC2Zu4cVl3Xp2-9BVFFKi51oytHzUZqX1ybY--0Nyiizl7EHzFAZJtnt7hyerSsIvt-DaHMBYsGxtzu6pFekktCLL1pzc5yYO0DMxz4qh9ERg4fBXhdpnTiWf2z6re99UogLQvCLgvwOu0_9nkZZNeH&uniplatform=NZKPT&language=CHS.
£Û23£Ý ZHANG K,HAN XL,HU WB,et al.miR-29a-3p inhibits the malignant characteristics of non-small cell lung cancer cells by reducing the activity of the Wnt/¦Â-catenin signaling pathway £ÛJ£Ý.Oncol Lett,2022,24(4):379.
£Û24£Ý WANG X,LIU SS,CAO L,et al.miR-29a-3p suppresses cell proliferation and migration by downregulating IGF1R in hepatocellular carcinoma £ÛJ£Ý.Oncotarget,2017,8(49):86592-86603.
£Û25£Ý LIU S,LIU DJ,LIU JW,et al.miR-29a-3p promotes migration and invasion in ameloblastoma via Wnt/¦Â-catenin signaling by targeting catenin beta interacting protein £ÛJ£Ý.Head Neck,2021,43(12):3911-3921.
£Û26£Ý ZHANG ZP,GUO X,GUO XF,et al.MicroRNA-29a-3p delivery via exosomes derived from engineered human mesenchymal stem cells exerts tumour suppressive effects by inhibiting migration and vasculogenic mimicry in glioma£ÛJ£Ý.Aging (Albany NY),2021,13(4):5055-5068.
£Û27£Ý ZHU K,ZHANG Y,LI DD,et al.MiR-29a-3p mediates phosphatase and tensin homolog and inhibits osteoarthritis progression £ÛJ£Ý.Funct Integr Genomics,2024,24(2):54.
£Û28£Ý SUN G,ZHENG C,DENG Z,et al.TRAF5 promotes the occurrence and development of colon cancer via the activation of PI3K/AKT/NF-¦ÊB signaling pathways £ÛJ£Ý.J Biol Regul Homeost Agents,2020,34(4):1257-1268.
£Û29£Ý LIANG Z,LI XL,LIU SQ,et al.MiR-141-3p inhibits cell proliferation,migration and invasion by targeting TRAF5 in colorectal cancer £ÛJ£Ý.Biochem Biophys Res Commun,2019,514(3):699-705.
£Û30£Ý CHEN Z,ZHAO L,ZHAO F,et al.MicroRNA-26b regulates cancer proliferation migration and cell cycle transition by suppressing TRAF5 in esophageal squamous cell carcinoma £ÛJ£Ý.Am J Transl Res,2016,8(5):1957-1970.

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