|Table of Contents|

Expression and clinical significance of AURKA in colorectal cancer tissues

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 03
Page:
440-447
Research Field:
Publishing date:

Info

Title:
Expression and clinical significance of AURKA in colorectal cancer tissues
Author(s):
LIN Qian1; TAO Yiping1; SHI Hao1; WANG Yuxing2; TONG Xiyan1; YU Hongnyu1
1.Central Laboratory,Xinhua Hospital Affiliated to Dalian University,Liaoning Dalian 116044,China;2.Department of Critical Care Medicine,People's Hospital of Chengmai County,Hainan Chengmai 571900,China.
Keywords:
colorectal cancer; AURKA; miRNA; bioinformatics analysis; prognosis
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2025.03.013
Abstract:
Objective:To analyze the expression of Aurora Kinase A(AURKA) in colorectal cancer tissues and its correlation with clinical pathological features of patients,predict upstream target genes,and explore its clinical significance in colorectal cancer.Methods:Bioinformatics analysis was used to investigate the expression of AURKA in colorectal cancer tissues,and immunohistochemistry was employed to detect the expression of AURKA in colorectal cancer and adjacent tissues.The TargetScan database was screened for miRNAs targeting AURKA.PCR was used to detect the mRNA expression of AURKA and miRNAs in colorectal cancer and adjacent tissues,and analyze the relationship between them and clinical pathology.Results:Bioinformatics analysis and immunohistochemistry revealed that the expression level of AURKA in colorectal cancer was significantly higher than that in adjacent tissues(P＜0.05),and was correlated with patient vascular invasion,neural invasion,Ki-67 expression,and TP53 mutation status.Through the TargetScan database,miR-490-3p was identified as a possible upstream target gene of AURKA,and their expressions were negatively correlated in colorectal cancer.miR-490-3p was also associated with vascular invasion and TP53 mutation.Conclusion:AURKA is highly expressed in colorectal cancer and may participate in the occurrence and development of colorectal cancer through the regulation of miR-490-3p,potentially serving as a marker and therapeutic target for colorectal cancer.

References:

［1］ SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020: globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
[2] MARMOL I,SANCHEZ-DE-DIEGO C,PRADILLA DIESTE A,et al.Colorectal carcinoma:a general overview and future perspectives in colorectal cancer［J］.Int J Mol Sci,2017,18(1):197.
[3] ZHANG Y,WANG Y,ZHANG B,et al.Methods and biomarkers for early detection,prediction,and diagnosis of colorectal cancer［J］.Biomed Pharmacother,2023,163:114786.
[4] BILLER LH,SCHRAG D.Diagnosis and treatment of metastatic colorectal cancer:a review［J］.JAMA,2021,325(7):669-685.
[5] JIANG M,LIU S,LIN J,et al.A pan-cancer analysis of molecular characteristics and oncogenic role of hexokinase family genes in human tumors［J］.Life Sci,2021,264:118669.
[6] XIAO X,ZHANG Z,LUO R,et al.Identification of potential oncogenes in triple-negative breast cancer based on bioinformatics analyses［J］.Oncol Lett,2021,21(5):363.
[7] DU R,HUANG C,LIU K,et al.Targeting AURKA in cancer:molecular mechanisms and opportunities for cancer therapy［J］.Mol Cancer,2021,20(1):15.
[8] CIARDIELLO F,CIARDIELLO D,MARTINI G,et al.Clinical management of metastatic colorectal cancer in the era of precision medicine［J］.CA Cancer J Clin,2022,72(4):372-401.
[9] WOLF AMD,FONTHAM ETH,CHURCH TR,et al.Colorectal cancer screening for average-risk adults:2018 guideline update from the American Cancer Society［J］.CA Cancer J Clin,2018,68(4):250-281.
[10] YAN M,WANG C,HE B,et al.Aurora-A Kinase:a potent oncogene and target for cancer therapy［J］.Med Res Rev,2016,36(6):1036-1079.
[11] BERDNIK D,KNOBLICH JA.Drosophila Aurora-A is required for centrosome maturation and actin-dependent asymmetric protein localization during mitosis［J］.Curr Biol,2002,12(8):640-644.
[12] ZHANG J,LI B,YANG Q,et al.Prognostic value of Aurora kinase A(AURKA) expression among solid tumor patients:a systematic review and meta-analysis［J］.Jpn J Clin Oncol,2015,45(7):629-636.
[13] DUAN Z,CHINN D,TU MJ,et al.Novel synergistic combination of mitotic arrest and promotion of apoptosis for treatment of pancreatic adenocarcinoma［J］.Transl Oncol,2019,12(4):683-692.
[14] SONG X,DU R,GUI H,et al.Identification of potential hub genes related to the progression and prognosis of hepatocellular carcinoma through integrated bioinformatics analysis［J］.Oncol Rep,2020,43(1):133-146.
[15] HONG OY,KANG SY,NOH EM,et al.Aurora kinase A inducesmigration and invasion by inducing epithelial-to-mesenchymal transition in colon cancer cells［J］.Beb Rep,2022,55(2):87-91.
[16] WANG LH,XIANG J,YAN M,et al.The mitotic kinase Aurora-A induces mammary cell migration and breast cancer metastasis by activating the Cofilin-F-actin pathway［J］.Cancer Res,2010,70(22):9118-9128.
[17] CHEN H,HU J,XIONG X,et al.AURKA inhibition induces Ewing's sarcoma apoptosis and ferroptosis through NPM1/YAP1 axis［J］.Cell Death Dis,2024,15(1):99.
[18] XIE Y,ZHU S,ZHONG M,et al.Inhibition of Aurora Kinase A induces necroptosis in pancreatic carcinoma［J］.Gastroenterology,2017,153(5):1429-1443.e5.
[19] LI SS,ZHU HJ,LI JY,et al.MiRNA-875-3p alleviates the progression of colorectal cancer via negatively regulating PLK1 level［J］.Eur Rev Med Pharmacol Sci,2020,24(3):1126-1133.
[20] KABEKKODU SP,SHUKLA V,VARGHESE VK,et al.Clustered miRNAs and their role in biological functions and diseases［J］.Biol Rev Camb Philos Soc,2018,93(4):1955-1986.
[21] GUO L,FU J,SUN S,et al.MicroRNA-143-3p inhibits colorectal cancer metastases by targeting ITGA6 and ASAP3［J］.Cancer Sci,2019,110(2):805-816.
[22] GAO X,LAI Y,ZHANG Z,et al.Long non-coding RNA RP11-480I12.5 promotes the proliferation,migration,and invasion of breast cancer cells through the miR-490-3p-AURKA-Wnt/β-Catenin axis［J］.Front Oncol,2020,10:948.
[23] ZHANG H,BAO J,ZHAO S,et al.MicroRNA-490-3p suppresses hepatocellular carcinoma cell proliferation and migration by targeting the aurora kinase A gene(AURKA) ［J］.Arch Med Sci,2019,16(2):395-406.
[24] WANG H,YANG G,YU Y,et al.MicroRNA-490-3p suppresses the proliferation and invasion of hepatocellular carcinoma cells via targeting TMOD3［J］.Oncol Lett,2020,20(4):95.

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辽宁省大连市中医药科学研究计划项目（编号:22Z 11015）
Last Update: 1900-01-01