|Table of Contents|

The role of berberine in proliferation,migration,apoptosis and autophagy of bladder cancer cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2025 03
Page:
367-374
Research Field:
Publishing date:

Info

Title:
The role of berberine in proliferation,migration,apoptosis and autophagy of bladder cancer cells and its mechanism
Author(s):
ZHAO Yuwan; GAO Sheng; MIAO Bailiang; QIU Jingping; ZHU Lugang; LIU Jianjun
Department of Urology,Affiliated Hospital of Guangdong Medical University,Guangdong Zhanjiang 524002,China.
Keywords:
berberine; bladder cancer; apoptosis; autophagy
PACS:
R737.14
DOI:
10.3969/j.issn.1672-4992.2025.03.003
Abstract:
Objective:To explore the anticancer effect of berberine (BBR) in bladder cancer cells and its mechanism.Methods:The inhibitory effect of BBR on the proliferation of bladder cancer cells was analyzed by CCK-8 assay and clonal formation assay,the migration ability of bladder cancer cells was detected by scratch assay and transwell assay,apoptosis detection kit was used to detect the changes of apoptosis,MDC kit and electron microscope were used to detect the changes of intracellular autophagosomes.Western blot was used to analyze the changes of Cleaved-PARP,Bax,Cleaved-caspase 9,Cleaved-caspase 3,and Bcl2,LC3B,Beclin1,P62,P-AKT and P-mTOR protein levels.Results:CCK-8 and clonal formation experiments showed that BBR significantly inhibited the proliferation of bladder cancer cells.Both scratch and Transwell experiments showed that BBR could inhibit the growth of bladder cancer cells by inhibiting their migration ability.BBR could depolarize the mitochondrial membrane of bladder cancer cells and induced apoptosis of bladder cancer cells through Caspase cascade.The intracellular autophagosomes were observed by MDC staining and electron microscopy,and it was found that BBR promoted autophagy in bladder cancer cells.By analyzing the expression levels of autophagy related proteins,it was further confirmed that BBR induced autophagy in bladder cancer cells.The expression levels of P-AKT and P-mTOR decreased in bladder cancer cells treated with BBR,suggesting that BBR can inhibit cell growth by inhibiting the AKT/m-TOR pathway in bladder cancer cells.Conclusion:BBR can significantly inhibit the proliferation and migration of bladder cancer cells,and induce apoptosis and autophagy.Moreover,BBR can inhibit the growth of bladder cancer cells by inhibiting the AKT/mTOR signaling pathway.

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