|Table of Contents|

Effect of ampelopsin on proliferation,apoptosis,invasion and migration of osteosarcoma cells by regulating the PPARγ signaling pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 22
Page:
4284-4289
Research Field:
Publishing date:

Info

Title:
Effect of ampelopsin on proliferation,apoptosis,invasion and migration of osteosarcoma cells by regulating the PPARγ signaling pathway
Author(s):
QUAN Bibo; LI Huaijing
Nanyang Medical College,Henan Nanyang 473000，China.
Keywords:
ampelopsin; PPARγ signaling pathway; osteosarcoma cells; biological behavior
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2024.22.011
Abstract:
Objective:To investigate the effect of ampelopsin (AMP) on the biological behavior of osteosarcoma cells by regulating the peroxisome proliferator-activated receptor γ (PPARγ) signaling pathway.Methods:U2OS cells with good growth were separated into six groups:Blank group,20 μmol/L AMP group,40 μmol/L AMP group,80 μmol/L AMP group,80 μmol/L AMP+GW9662 (PPARγ inhibitor) group,and 80 μmol/L AMP+ROZ (PPARγ activator-rosiglitazone) group.CCK-8 method and clone formation experiment were applied to detect cell proliferation.Transwell and scratch experiments were applied to detect the invasion and migration of U2OS cells.Flow cytometry was applied to detect U2OS cell apoptosis.Western blot was applied to detect the expression levels of Bcl-2,PCNA,MMP-9,and PPARγ proteins.Results:Compared with the blank group,the proliferation,clone number,invasion,migration rates,Bcl-2,PCNA,and MMP-9 expression of U2OS cells in the 20 μmol/L AMP group,40 μmol/L AMP group,and 80 μmol/L AMP group obviously reduced,the apoptosis rate and PPARγ expression obviously increased (P＜0.05).Compared with the 80 μmol/L AMP group,the proliferation,clone number,invasion,migration rates,Bcl-2,PCNA,and MMP-9 expression of U2OS cells in the 80 μmol/L AMP+GW9662 group obviously increased,the apoptosis rate and PPARγ expression significantly reduced (P＜0.05).However,the proliferation,clone number,invasion,migration rates,Bcl-2,PCNA,and MMP-9 expression of U2OS cells in the 80 μmol/L AMP+ROZ group significantly reduced,the apoptosis rate and PPARγ expression obviously increased (P＜0.05).Conclusion:AMP inhibits the malignant biological behavior of osteosarcoma cells by activating the PPARγ signaling pathway.

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