|Table of Contents|

Impacts of circBACH1 on proliferation,apoptosis,and invasion of osteosarcoma cells by regulating the miR-101-3p/KPNA2 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 22
Page:
4254-4261
Research Field:
Publishing date:

Info

Title:
Impacts of circBACH1 on proliferation,apoptosis,and invasion of osteosarcoma cells by regulating the miR-101-3p/KPNA2 axis
Author(s):
ZHANG Lixi1; YANG Jian2; SHI Bo3
1.Department of Orthopedics,Tangshan Fengnan District Hospital,Hebei Tangshan 063300,China;2.Department of Orthopedics,Cangzhou Central Hospital,Hebei Cangzhou 061000,China;3.Department of Orthopedics,Cangzhou People's Hospital,Hebei Cangzhou 061000,China.
Keywords:
circular RNA BACH1; microRNA-101-3p; nuclear transporter protein karyopherin α2; osteosarcoma; proliferation; apoptosis; invasion
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2024.22.007
Abstract:
Objective:To explore the impacts of circular RNA BACH1 (circBACH1) on proliferation,apoptosis,and invasion of osteosarcoma (OS) cells by targeting microRNA-101-3p (miR-101-3p)/nuclear transporter protein karyopherin α2 (KPNA2) axis.Methods:The qRT-PCR method was applied to analyze the expression levels of circBACH1 and miR-101-3p in OS tissues.Dual Luciferase was applied to detect the targeting relationship between circBACH1 and miR-101-3p,and between KPNA2 and miR-101-3p.The corresponding plasmid transfected in OS cells MG-63 was labeled si-NC group,si-circBACH1 group,si-circBACH1+anti-NC group,si-circBACH1+anti-miR-101-3p group,miR-NC group,miR-101-3p mimics group,and miR-101-3p mimics+pcDNA group,miR-101-3p mimics+KPNA2 group.Plate cloning was applied to detect cell proliferation.Annexin V-FITC/PI method was applied to detect cell apoptosis.Transwell experiment was applied to detect cell invasion ability.Western blot was applied to detect the protein expression changes of cyclin D1,C-caspase-3,E-cadherin,N-cadherin and Vimentin.Mouse transplantation tumor experiment was applied to verify the effect of circBACH1 on OS tumor growth and miR-101-3p/KPNA2.Results:In OS tissues,circBACH1 expression was up-regulated and miR-101-3p expression was down-regulated (P＜0.05).Inhibiting the expression of circBACH1 was able to obviously inhibit the proliferation and invasion of OS cells,and induce apoptosis (P＜0.05).Inhibition of miR-101-3p expression can promote the proliferation and invasion of OS cells and inhibit cell apoptosis (P＜0.05).Overexpression of miR-101-3p can down-regulate the expression of KPNA2,inhibit the proliferation and invasion of OS cells,and induce apoptosis (P＜0.05).Overexpression of KPNA2 was able to reverse the inhibitory effect of overexpression of miR-101-3p on OS cell proliferation and invasion (P＜0.05).The results of the mouse transplantation tumor experiment showed that inhibiting the expression of circBACH1 resulted in a decrease in the volume and quality of the transplanted tumor,an increase in the expression level of miR-101-3p,and a decrease in the expression level of KPNA2 (P＜0.05).Conclusion:circBACH1 is highly expressed in OS tissues,and inhibition of circBACH1 expression can inhibit the proliferation and invasion of OS cells and induce apoptosis,which is related to the regulation of miR-101-3p/KPNA2 axis.

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