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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 22

Page:

4235-4244

Research Field:

Publishing date:

Info

Title:

Expression of CST6 in thyroid papillary carcinoma and its effect on cell biological function

Author(s):

HU Jinbo¹; ZHANG Lu²; NIU Haigang¹; 3; GUO Wenke³; TIAN Xiaoai³; GUO Jianping³

1.Fenyang College of Shanxi Medical University,Shanxi Lvliang 032200,China;2.Affiliated Hospital of Hebei University,Hebei Baoding 071000,China；3.Fenyang Hospital,Shanxi Province,Shanxi Lvliang 032200,China.

Keywords:

cysteine protease inhibitory factor 6; papillary thyroid carcinoma; cervical lymph node metastasis; epithelial-mesenchymal transition

PACS:

R736.1

DOI:

10.3969/j.issn.1672-4992.2024.22.004

Abstract:

Objective:To study the expression of cysteine protease inhibitory factor 6 (Cystatin M/E,Cystatin 6,CST6) in papillary thyroid carcinoma (PTC) and its correlation with cervical lymph node metastasis,and the relationship between the expression of CST6 and the malignant biological behavior of PTC cells,and initially explore its pathway.relationship between CST6 expression and the malignant biological behavior of PTC cells,and preliminarily explore its pathway of action.Methods:119 PTC patients were selected,and immunohistochemistry was applied to detect CST6 protein expression in PTC and thyroid follicular epithelial tissues,and the protein expression level of CST6 was detected by RT-qPCR and Western Blot in human papillary thyroid carcinoma cell lines TPC-1,K1,and B-CPAP,as well as in human thyroid normal cell line Nthy-ori 3-1.CST6 overexpressing and interfering cell lines were screened and constructed according to the expression status,and changes in the invasion and migration ability of PTC cells were determined by Transwell and Wound-Healing assays.The change trend of key proteins (E-cadherin,N-cadherin,Vimentin) in epithelial-mesenchymal transition (EMT) was detected by Western Blot.Results:Compared with normal thyroid tissues,CST6 was expressed at a lower level in PTC tissues,and CST6 was highly expressed in the follicular epithelium of the thyroid gland of patients with pN1 versus those of thyroid cancer tissues of patients with pN0.The expression level of CST6 was correlated with the length of the tumor diameter (P＜0.000 1) as well as with the metastasis of the cervical lymph nodes (P＜0.000 3).Compared with the OE-CST6 group,the sh-CST6 group had a stronger trend of invasion and migration.Meanwhile,E-cadherin protein expression was significantly down-regulated and N-cadherin and Vimentin protein expression was significantly up-regulated in sh-CST6 group (P＜0.05).Conclusion:CST6,as a protective factor,was lowly expressed in PTC,and its expression degree was correlated with lymph node metastasis.Overexpression of CST6 protein could inhibit the migration and invasion ability of PTC cells,as well as reduce cervical lymph node metastasis caused by the trans-epithelial-mesenchymal transition pathway.

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山西省吕梁市重点研发项目(编号：2020SHFZ63)；山西省汾阳医院科技攻关项目(编号：202305)

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