|Table of Contents|

Expression and prognostic significance of mitochondrial transcription elongation factor in human brain glioma tissues

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 21
Page:
4070-4076
Research Field:
Publishing date:

Info

Title:
Expression and prognostic significance of mitochondrial transcription elongation factor in human brain glioma tissues
Author(s):
LIN Yaru1; 2; WANG Xinmeng1; 2; ZI Jiaji1; 2; PU Yuanqian1; 2; DONG Baoru3; YU Min4; XIONG Wei1; 2
1.College of Basic Medical Sciences,Yunnan Dali 671000,China;2.Yunnan Provincial Key Laboratory of Entomological Biopharmaceutica,College of Pharmacy,Dali University,Yunnan Dali 671000,China;3.Department of General Surgery,the Second People’s Hospital of Baoshan,Yunnan Baoshan 678000,China;4.College of Life Sciences,Yunnan University,Yunnan Kunming 650091,China.
Keywords:
mitochondrial transcription elongation factor; brain glioma; immunohistochemistry; prognosis
PACS:
R739.41
DOI:
10.3969/j.issn.1672-4992.2024.21.010
Abstract:
Objective:To investigate the expression of human mitochondrial transcription elongation factor(TEFM) in glioma tissues and its relationship with clinicopathological features and prognosis．Methods:From January 2019 to June 2021，68 cases of glioma tissues and 35 cases of non-tumorous brain tissues surgical resected and confirmed by pathology were collected.Immunohistochemistry and Western blot was used to determine the expressions of TEFM in glioma tissues and non-tumorous brain tissues.Real-time fluorescence quantitative RT-PCR (qRT-PCR) was used to detect the mRNA level of TEFM in the above tissues.The expression and distribution of TEFM mRNA in different clinicopathological features of glioma (age,sex,pathological type,pathological grade,history of headache,laterality,and supratentorial location) were analyzed.The relationship between TEFM mRNA level and prognosis was analyzed according to follow-up data．Results:Immunohistochemical analysis revealed that TEFM protein is predominantly localized in the cytoplasm.The positive expression rate of TEFM in various grades of brain glioma tissues (91.18%) significantly surpassed that in non-tumor brain tissues (37.14%),showing a highly significant intergroup difference (P＜0.01).Western blot and qRT-PCR results showed that both TEFM protein and mRNA were significantly increased in glioma tissue (P＜0.01).The expression of TEFM gene in glioma patients was significantly correlated with age,pathological grade,pathological type,laterality and supratentorial location (P＜0.05).Kaplan-Meier survival analysis showed that the expression of TEFM gene significantly affected the overall survival(OS) and disease-free survival(DFS) of glioma patients,while the OS and DFS of patients with high expression of TEFM were significantly shortened (Log-rank P＜0.05).Using the COX regression model to analyze the independent risk factors for the prognosis of patients with gliomas,age,pathological grade,pathological type and IDH1 mutation status are independent risk factors that affect the prognosis of glioma patients (P＜0.05).Conclusion:TEFM is significantly up-regulated in human brain glioma tissues compared to non-tumorous brain tissues.Patients with high TEFM expression have significantly shorter OS and DFS.

References:

［1］LI J,MENG Q,ZHOU X,et al.Gospel of malignant glioma:oncolytic virus therapy［J］.Gene,2022,818:146217.
［2］KOMORI T.The 2016 WHO classification of tumours of the central nervous system:the major points of revision［J］.Neurol Med Chir(Tokyo),2017,57(7):301-311.
［3］GHIASEDDIN AP,SHIN D,MELNICK K,et al.Tumor treating fields in the management of patients with malignant gliomas［J］.Curr Treat Options Oncol,2020,21(9):76.
［4］权俊杰,屈建强,周乐.肝细胞再生磷酸因子-1在脑胶质瘤组织中的表达及对脑胶质瘤细胞凋亡的影响［J］.河北医学,2017,23(5):724-728.QUAN JJ,QU JQ,ZHOU L.Expression of hepatocyte growth factor-1 in human glioma and effect on apoptosis of glioma cells［J］.Hebei Medicine,2017,23(5):724-728.
［5］BRLEK P,KAFKA A,BUKOVAC A,et al.Integrative cBioPortal analysis revealed molecular mechanisms that regulate EGFR-PI3K-AKT-mTOR pathway in diffuse gliomas of the brain［J］.Cancers(Basel),2021,13(13):3247.
［6］孙晓梅,梅雯,何冰,等.Ki-67、Bcl-6、Bcl-2及TEFM在弥漫大B细胞淋巴瘤中的表达及预后意义［J］.大理大学学报,2023,8(2):62-69.SUN XM,MEI W,HE B,et al.Expression and prognostic significance of Ki-67,Bcl-6,Bcl-2 and TEFM in diffuse large B cell lymphoma［J］.Journal of Dali University,2023,8(2):62-69.
［7］李素芬,余敏,熊伟.线粒体转录延伸因子：调控线粒体DNA复制与转录转换的分子开关［J］.中国生物化学与分子生物学报,2020,36(5):504-511.LI SF,YU M,XIONG W.Mitochondrial transcription elongation factors:a replication-transcription switch in human mitochondria［J］.Chinese Journal of Biochemistry and Molecular Biology,2020,36(5):504-511.
［8］王唯斯,李翔宇,郝西琳,等.基于TCGA数据集分析脑胶质瘤中TEFM基因mRNA的表达及意义［J］.解放军医药杂志,2019,31(5):15-21.WANG WS,LI XY,HAO XL,et al.Expression and significance of TEFM gene in brain glioma on the basis of TCGA dataset analysis［J］.Medical & Pharmaceutical Journal of Chinese People’s Liberation Army,2019,31(5):15-21.
［9］VAN HAUTE L,O’CONNOR E,DIAZ-MALDONADO H,et al.TEFM variants impair mitochondrial transcription causing childhood-onset neurological disease［J］.Nat Commun,2023,14(1):1009.
［10］李素芬,李彬,普元倩,等.肝细胞性肝癌中线粒体转录延伸因子的表达及意义［J］.临床与实验病理学杂志,2021,37(8):911-916.LI SF,LI B,PU YQ,et al.Expression of mitochondrial transcription elongation factor in hepatocellular carcinoma and its significance［J］.Chinese Journal of Clinical and Experimental Pathology,2021,37(8):911-916.
［11］POSSE V,SHAHZAD S,FALKENBERG M,et al.TEFM is a potent stimulator of mitochondrial transcription elongation factor in vitro［J］.Nucleic Acid Res,2015,43(5):2615-2624.
［12］MINCZUK M,HE J,DUCH AM,et al.TEFM (c17ofr42) is necessary for transcription of human mtDNA［J］.Nucleic Acid Res,2011,39(10):4284-4299.
［13］王燕,夏小鹏,何鑫,等.HAX1及VEGFA表达与脑胶质瘤预后的相关性及临床价值［J］.临床与实验病理学杂志,2023,39(1):29-36.WANG Y,XIA XP,HE X,et al.Expression and clinical significance of HAX1 and VEGFA in human glioma［J］.Chinese Journal of Clinical and Experimental Pathology,2023,39(1):29-36.
［14］及时雨,蒋国梁,齐平建,等.脑胶质瘤组织中OCT4A和OCT4B水平及临床意义分析［J］.临床肿瘤学杂志,2018,23(10):893-899.JI SY,JIANG GL,QI PJ,et al.Levels and clinical significance of OCT4A and OCT4B in glioma tissues［J］.Chinese Clinical Oncology,2018,23(10):893-899.
［15］丁玉召,符静波,郑喜胜,等.脑 胶质瘤组织中的circSORBS1水平及临床意义分析［J］.临床肿瘤学杂志,2023,28(4):360-364.DING YZ,FU JB,ZHENG XS,et al.Expression and clinical significance of circSORBS1 in glioma tissues［J］.Chinese Clinical Oncology,2023,28(4):360-364.
［16］VERBURG N, POUWELS PJ,BOELLAARD R,et al.Accurate delineation of glioma infiltration by advanced PET/MR neuro-imaging (fron-tier study):a diagnostic study protocol［J］.Neurosurgery,2016,79(4):535-540.
［17］GHOUZLANI A,KANDOUSSI S,TALL M,et al.Immune checkpoint inhibitors in human glioma microenvironment［J］.Front Immunol,2021,12:679425.
［18］MAIR DB,AMES HM,LI R.Mechanisms of invasion and motility of high-grade gliomas in the brain［J］.Mol Biol Cell,2018,29(21):2509-2515.
［19］林树楷,李钢,周奋,等.脑胶质瘤组织中lncRNA-LINC01116的表达变化及其意义［J］.山东医药,2018,58(27):1-4.LIN SK,LI G,ZHOU F,et al.Change in expression of lncRNA-LINC01116 in brain glioma［J］.Shandong Medical Journal,2018,58(27):1-4.
［20］ZENG Z,CHEN Y,GENG X,et al.NcRNAs:multi angle participation in the regulation of glioma chemotherapy resistance (review)［J］.Int J Oncol,2022,60(6):76.
［21］LI S,WANG W,ZI J,et al.Elevated expression of mitochondrial transcription elongation factor (TEFM) predicts poor prognosis in low grade glioma-an analysis of the cancer genome atlas(TCGA) dataset［J］.Transl Cancer Res,2020,9(5):3610-3622.
［22］LUO Y,MA J,LU W.The significance of mitochondrial dysfunction in cancer［J］.Int J Mol Sci,2020,21(16):5598.
［23］SOON BH,ABU N,ABDUL MN,et al.Somatic mitochondrial DNA mutations in different grades of glioma［J］.Per Med,2022,19(1):25-39.
［24］SRINIVASAN S,GUHA M,KASHINA A,et al.Mitochondrial dysfunction and mitochondrial dynamics-the cancer connection［J］.Biochim Biophys Acta Bioenerg,2017,1858(8):602-614.
［25］WAN L,WANG Y,ZHANG Z,et al.Elevated TEFM expression promotes growth and metastasis through activation of ROS/ERK signaling in hepatocellular carcinoma［J］.Cell Death Dis,2021,12(4):325.
［26］FEI Z,WANG W,LI S,et al.High expression of the TEFM gene predicts poor prognosis in hepatocellular carcinoma［J］.J Gastrointest Oncol,2020,11(6):1291-1304.

Memo

Memo:
National Natural Science Foundation of China（No.32160167,82160516）;国家自然科学基金项目(编号：32160167,82160516)；云南省应用基础研究专项面上项目(编号：202201AT070004，202301AT070023)；云南省昆虫生物医药研发重点实验室开放课题(编号：AG202203,AP2022006)；云南省教育厅科学研究基金项目(编号：2024Y875)
Last Update: 2024-09-30