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Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 21

Page:

4028-4035

Research Field:

Publishing date:

Info

Title:

Preparation and in vitro cell imaging study of fluorescent probe liposomes responsive to myeloperoxidase

Author(s):

DUAN Jiangbo¹; 2; TAO Jianlin³; ZHU Jiang⁴; HOU Yiling¹; 2

Keywords:

myeloperoxidase; luminol; Cy5; fluorescent probe liposomes; cell imaging

PACS:

R741.044

DOI:

10.3969/j.issn.1672-4992.2024.21.003

Abstract:

Objective:To prepare luminol-Cy5 fluorescent probe liposomes which responsive to myeloperoxidase,optimize the preparation process and explore the in vitro cell imaging effect.Methods:Using lecithin,cholesterol,and collagen as raw materials of liposomes,luminol-Cy5 fluorescent probe liposomes were prepared by thin film hydration method.The preparation process was optimized using single factor analysis and response surface methodology.At the same time,RAW264.7 mouse macrophage cells that showed inflammation after LPS stimulation were used as the model to explore the cellular imaging effect of fluorescent probe liposomes responsive to myeloperoxidase.Results:The results showed that when the mass ratio of luminol to Cy5 was 2¡Ã1,the film material ratio was 4¡Ã1 and the hydration temperature was 40 ¡æ,the fluorescence probe liposome encapsulation effect was the best,with a luminol encapsulation efficiency of 57.26% and a Cy5 encapsulation efficiency of 60.22%.The average particle size of luminol-Cy5 fluorescent probe liposome was 205.01 nm,and the Zeta potential was -30.41 mV.The cell imaging results showed that in RAW264.7 mouse macrophages stimulated by LPS,luminol in the fluorescent probe liposome was able to respond to myeloperoxidase activity and emit blue fluorescence.At the same time,this blue fluorescence could stimulate Cy5 in the fluorescent liposome to emit red fluorescence at 677 nm,achieving imaging detection of deep inflammatory cells.Conclusion:Luminol-Cy5 fluorescent probe liposomes could respond to myeloperoxidase in vitro and achieve imaging detection of deep inflammatory cells through bioluminescence resonance energy transfer (BRET).They had certain application value in the evaluation and treatment of inflammatory reactions caused by tumors and epilepsy.

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