|Table of Contents|

The expression and clinical significance of microRNA-576-3p and microRNA-766-5p in the serum of patients with epithelial ovarian cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3915-3920
Research Field:
Publishing date:

Info

Title:
The expression and clinical significance of microRNA-576-3p and microRNA-766-5p in the serum of patients with epithelial ovarian cancer
Author(s):
ZHANG Yingying1; LYU Lu2; CHEN Ru2; SUN Lijuan2; HU Xiaojing2; CUI Hongying2
1.Department of Gynecology;2.Department of Gynecology and Obstetrics,the People's Hospital of Hebi,Henan Hebi 458030,China.
Keywords:
epithelial ovarian cancer; microRNA-576-3p; microRNA-766-5p; diagnostic value
PACS:
R737.31
DOI:
10.3969/j.issn.1672-4992.2024.20.018
Abstract:
Objective:In order to improve the diagnostic accuracy of patients with epithelial ovarian cancer (EOC) in clinical practice,the expression and clinical significance of microRNA-5766-3p (miR-576-3p) and microRNA-766-5p (miR-766-5p) in the serum of EOC patients were investigated.Methods:A total of 103 EOC patients admitted to our hospital from August 2017 to August 2022 were regarded as the study group,103 patients with ovarian cysts were selected as the benign control group,and another 103 healthy individuals who underwent physical examination at our hospital during the same period were selected as the healthy control group.Measure and analyze the expression levels and correlation of serum miR-576-3p and miR-766-5p in patients,and explore the influencing factors related to the occurrence of EOC.Evaluate the diagnostic value of miR-576-3p and miR-766-5p for EOC using receiver operating characteristic curve (ROC).Results:There was a significant difference in PLR and NLR values among the three groups(χ2=151.671,69.059,P＜0.05).The serum miR-576-3p expression level in the study group (0.76±0.22) was lower than that in the healthy control group (1.02±0.17) and benign control group (0.92±0.12) (F=57.959,P＜0.001),while the miR-766-5p expression level in the study group (1.24±0.45) was significantly higher than that in the healthy control group (1.01±0.11) and benign control group (1.11±0.31) (F=13.227,P＜0.001),and the serum miR-576-3p expression level increased (0.89±0.11) (t=5.364,P＜0.001) after treatment,and the expression level of serum miR-766-5p decreased (1.11±0.35) (t=2.314,P＜0.05).The serum miR-576-3p expression levels in EOC patients with FIGO stage III+IV (0.58±0.16),lymph node metastasis (0.60±0.18),and poor degree of differentiation (0.63±0.20) were lower than those in EOC patients with FIGO stage I+II (0.87±0.26),no lymph node metastasis (0.85±0.35),and well degree of differentiation (0.82±0.31) (P＜0.05),while the trend of serum miR-766-5p expression levels was opposite.PLR (OR=2.114),NLR (OR=1.594),and miR-766-5p (OR=1.730) were all risk factors for EOC,while miR-576-3p (OR=0.627) was a protective factor (P＜0.05).The area under the curve (AUC) of serum miR-576-3p,miR-766-5p,PLR,NLR,and combined detection for diagnosing EOC was 0.801 (95%CI=0.739~0.853),0.741 (95%CI=0.676~0.800),0.906 (95%CI=0.857~0.942),0.776 (95%CI=0.713~0.831),and 0.970 (95%CI=0.937~0.989),respectively.Joint detection was superior to individual detection (Zcombined vs miR-576-3p=6.149,Zcombined vs miR-766-5p=6.300,Zcombined vs PLR=3.550,Zcombined vs NLR=5.592,P＜0.001).Conclusion:There is a certain correlation between the expression levels of serum miR-576-3p and miR-766-5p and the occurrence of EOC.The combined detection has high sensitivity in diagnosing EOC and has certain application value in assisting clinical diagnosis of EOC.

References:

［1］ 李由由,卢丹.上皮性卵巢癌组织中配对盒基因8、波形蛋白表达与临床病理特征及化疗后复发的关系［J］.实用临床医药杂志,2023,27(18):1-4,10. LI YY,LU D.The relationship between the expression of pairing box gene 8 and vimentin in epithelial ovarian cancer tissue and clinical pathological features,as well as recurrence after chemotherapy ［J］.Journal of Practical Clinical Medicine,2023,27(18):1-4,10.
［2］ 刘坤,焦洪斌,杨青.磁共振弥散加权成像联合糖类抗原125对卵巢交界性肿瘤与上皮性卵巢癌的鉴别诊断价值［J］.实用临床医药杂志,2023,27(5):31-36. LIU K,JIAO HB,YANG Q.The differential diagnostic value of diffusion-weighted magnetic resonance imaging combined with carbohydrate antigen 125 in ovarian borderline tumors and epithelial ovarian cancer ［J］.Journal of Practical Clinical Medicine,2023,27(5):31-36.
［3］ SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:Globocan estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA:A Cancer Journal for Clinicians,2021,71(3):209-249.
［4］ 侯芳,彭洁,李洁,等.上皮性卵巢癌患者miR-760,SOCS3表达及其临床意义［J］.中国性科学,2022,31(4):55-59. HOU F,PENG J,LI J,et al.The expression and clinical significance of miR-760 and SOCS3 in patients with epithelial ovarian cancer［J］.Chinese Journal of Sexual Science,2022,31(4):55-59.
［5］ ZUO Y,ZHENG W,TANG Q,et al.miR-576-3p overexpression enhances cisplatin sensitivity of ovarian cancer cells by dysregulating PD-L1 and cyclin D1［J］.Molecular Medicine Reports,2021,23(1):81-82.
［6］ GAO E,LI Y,ZHAO W,et al.Necessity of thoracoto my in pulmonary metastasis of osteosarcoma［J］.Journal of Thoracic Disease,2019,11(8):3578-3583.
［7］ LI H,CAO B,ZHAO R,et al.circDNMT1 promotes malignant progression of gastric cancer through targeting miR-576-3p/Hypoxia inducible factor-1 alpha axis［J］.Front Oncol,2022,30(12):192-193.
［8］ 刘国成,官一平,王成,等.微小RNA-766-5p通过调控组氨酸磷酸酶基因表达影响胃癌细胞增殖和放射敏感性［J］.安徽医药,2022,26(2):274-278. LIU GC,GUAN YP,WANG C,et al.MicroRNA-766-5p affects gastric cancer cell proliferation and radiosensitivity by regulating the expression of histidine phosphatase gene［J］.Anhui Medicine,2022,26(2):274-278.
［9］ 周琦,吴小华,刘继红,等.卵巢恶性肿瘤诊断与治疗指南(第四版)［J］.中国实用妇科与产科杂志,2018,34(7):739-749. ZHOU Q,WU XH,LIU JH,et al.Guidelines for diagnosis and treatment of ovarian malignant tumors (fourth edition)［J］.Chinese Journal of Practical Gynecology and Obstetrics,2018,34(7):739-749.
［10］ 王卉,陈学平,陈运,等.ENTPD5在上皮性卵巢癌组织中高表达:基于Oncomine数据库及生物信息学方法［J］.南方医科大学学报,2021,41(4):555-561. WANG H,CHEN XP,CHEN Y,et al.High expression of ENTPD5 in epithelial ovarian cancer tissue:based on Oncomine database and bioinformatics methods［J］.Journal of Southern Medical University,2021,41(4):555-561.
［11］ 韦昕芳,熊丽丽,姜陵.上皮性卵巢癌患者血清中CENPU,ErbB2基因表达与患者临床病理特征及预后的关系［J］.实用癌症杂志,2022,37(7):1085-1088. WEI XF,XIONG LL,JIANG L.The relationship between the expression of CENPU and ErbB2 genes in the serum of patients with epithelial ovarian cancer and their clinical pathological characteristics and prognosis［J］.Journal of Practical Cancer,2022,37(7):1085-1088.
［12］ 霍叶琳,王月,安娜,等.TIM-3基因在上皮性卵巢癌中高表达并促进癌细胞的增殖和迁移［J］.南方医科大学学报,2022,42(2):190-200. HUO YL,WANG Y,AN N,et al.TIM-3 gene is highly expressed in epithelial ovarian cancer and promotes cancer cell proliferation and migration［J］.Journal of Southern Medical University,2022,42(2):190-200.
［13］ STALEY SA,TUCKER K,NEWTON M,et al.Sarcopenia as a predictor of survival and chemotoxicity in patients with epithelial ovarian cancer receiving platinum and taxane based chemotherapy［J］.Gynecol Oncol,2020,156(3):695-700.
［14］ 王思念,吕进,张巧云,等.miRNA与肿瘤侵袭转移［J］.现代肿瘤医学，2016,24(03):485-487. WANG Sinian,LYU Jin,ZHANG Qiaoyun，et al.miRNA and invasion and metastasis of cancer［J］.Modern Oncology,2016,24(03):485-487.
［15］ 徐蕾蕾,华馥,李长华,等.上皮性卵巢癌组织miR-338-3p,miR-1294的表达及临床意义［J］.现代生物医学进展,2020,20(22):4293-4297. XU LL,HUA F,LI CH,et al.The expression and clinical significance of miR-338-3p and miR-1294 in epithelial ovarian cancer tissue［J］.Modern Biomedical Progress,2020,20(22):4293-4297.
［16］ ZHOU ZG,LIU R,TAN HL,et al.The application of dexmedelomidine combined with dezocine in thoraco-scopic radical resection of lung cancer and its effect on awakening quality of patients［J］.European Review for Medical and Pharmacological Sciences,2019,23(17):7694-7702.
［17］ ELDALY MN,METWALLY FM,SHOU WG,et al.Clinical potentials of miR-576-3p,miR-613,NDRG2 and YKL40 in colorectal cancer patients［J］.Asian Pacific Journal of Cancer Prevention,2020,21(6):1689-1695.
［18］ 韩林育,王娜,张习军.circRNA-0000285靶向miR-576-3p调控宫颈癌细胞增殖,迁移,侵袭的分子机制［J］.中国优生与遗传杂志,2022,30(1):35-39. HAN LY,WANG N,ZHANG XJ.The molecular mechanism of circRNA-0000285 targeting miR-576-3p in regulating the proliferation,migration,and invasion of cervical cancer cells［J］.Chinese Journal of Eugenics and Genetics,2022,30(1):35-39.
［19］ 郭秀莲,邢海,王巍.miR-766-5p过表达介导MMP-7对胶质瘤细胞增殖,侵袭与凋亡的影响及机制［J］.医学分子生物学杂志,2022,19(5):360-365. GUO XL,XING H,WANG W.Overexpression of miR-766-5p mediates the effects and mechanisms of MMP-7 on proliferation,invasion,and apoptosis of glioma cell［J］.Journal of Medical Molecular Biology,2022,19(5):360-365.
［20］ YANG C,MA X,GUAN G,et al.MicroRNA-766 promotes cancer progression by targeting NR3C2 in hepatocellular carcinoma［J］.FASEB J,2019,33(1):1456-1467.
［21］ 梁芳,张昊,郜辉,等.外周血NLR联合血清AFP、GP73、DKK1检测对原发性肝癌的诊断价值［J］.现代肿瘤医学，2021,29(06):989-993. LIANG Fang,ZHANG Hao,GAO Hui,et al.Diagnostic value of peripheral blood NLR combined with serum AFP,GP73 and DKK1 in primary hepatocellular carcinoma［J］.Modern Oncology,2021,29(06):989-993.
［22］ ZHANG CL,JIANG XC,LI Y,et al.Independent predictive value of blood inflammatory composite markers in ovarian cancer:recent clinical evidence and perspective focusing on NLR and PLR［J］.Journal of Ovarian Research,2023,16(1):36-37.
［23］ 李飞霞,田刚,余婷,等.血清CA125,HE4联合PLR,NLR MLR诊断上皮性卵巢癌的临床价值［J］.山东医药,2020,60(10):70-72. LI FX,TIAN G,YU T,et al.The clinical value of serum CA125,HE4 combined with PLR,NLR MLR in the diagnosis of epithelial ovarian cancer［J］.Shandong Pharmaceutical,2020,60(10):70-72.
［24］ 陈玲,王衍,孙洪芳,等.基于术前NLR,LMR及白蛋白的炎症预后指数评估上皮性卵巢癌患者预后的价值［J］.现代肿瘤医学,2021,29(17):3069-3073. CHEN L,WANG Y,SUN HF,et al.The value of preoperative NLR,LMR,and albumin based inflammatory prognostic indices in evaluating the prognosis of epithelial ovarian cancer patients［J］.Modern Oncology,2021,29(17):3069-3073.
［25］ FAROLFI A,SCARPI E,GRECO F,et al.Inflammatory indexes as predictive factors for platinum sensitivity and as prognostic factors in recurrent epithelial ovarian cancer patients:a MITO24 retrospective study［J］.Scientific Reports,2020,10(1):1-2.

Memo

Memo:
河南省医学科技攻关计划项目(编号：2018020289)
Last Update: 1900-01-01