|Table of Contents|

Value of serum uMtCK detection in diagnosis of non-small cell lung cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3875-3879
Research Field:
Publishing date:

Info

Title:
Value of serum uMtCK detection in diagnosis of non-small cell lung cancer
Author(s):
LI Zhiwen1; XIN Qian2; LI Chenyu2; ZHANG Lei2; BI Qingqing2; MU Xiaofeng2
1.School of Medical Laboratory,Weifang Medical University,Shandong Weifang 261053,China;2.Department of Clinical Laboratory,Affiliated Qingdao Central Hospital of Qingdao University,Qingdao Cancer Hospital,Shandong Qingdao 266042,China.
Keywords:
uMtCK; CYFRA21-1; CEA; joint detection; NSCLC
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.20.010
Abstract:
Objective:To investigate the value of serum ubiquitous mitochondrial creatine kinase (uMtCK) level for clinical diagnosis and prognostic monitoring of non-small cell lung cancer (NSCLC),and the value of the combination of serum uMtCK,cytokeratin fragment 19 (CYFRA21-1) and carcino-embryonic antigen (CEA) for diagnosis of NSCLC.Methods:NSCLC patients from March 2023 to August 2023 were included as the experimental group of this study,and the healthy check-up population during the same period was used as the control group,and the levels of serum uMtCK,CYFRA21-1,and CEA of two groups were detected by chemiluminescence immunoassay (CLIA) to evaluate the differences.The diagnostic accuracy of single or combined detection of uMtCK,CYFRA21-1,CEA in NSCLC patients was analyzed by ROC curve.Results:A total of 149 newly discovered NSCLC patients,130 treated NSCLC patients and 85 healthy controls were included in this study.Serum uMtCK levels of overall,male,and female NSCLC patients were significantly higher than those of the corresponding control populations (42.30 vs 10.10,34.40 vs 8.40,62.90 vs 14.3,P＜0.05).ROC curve analysis showed that the AUC of the uMtCK assay for the total population,male and female patients was 0.818,0.834 and 0.828,respectively.The AUC of the combined uMtCK,CYFRA21-1,and CEA assays for the total population,male and female patients was 0.913,0.931 and 0.909,respectively,which was higher than the AUC of the three markers alone.The log-transformed multifactorial Logistic regression analysis showed that the OR of uMtCK was 3.09,significantly higher than that of CYFRA21-1 and CEA,and the AUC of the log-transformed multifactorial Logistic regression model was 0.954.Disease staging study showed that the serum expression level of uMtCK in patients with stage Ⅰ-Ⅱ was higher than that of patients with stage Ⅲ-Ⅳ (P＜0.05).The level of uMtCK was lower in surgically treated stage I-II patients than in untreated stage I-II patients,and the difference was statistically significant (P＜0.05).Conclusion:Serum uMtCK level can be used as an auxiliary diagnosis for NSCLC,especially for early lung cancer,and can be used to monitor the postoperative efficacy of early-stage patients with NSCLC.The combination of uMtCK,CYFRA21-1,and CEA and the log-transformed multifactor Logistic regression model can effectively improve the diagnostic efficacy of NSCLC.

References:

［1］ SIEGEL RL,MILLER KD,WAGLE NS,et al.Cancer statistics,2023［J］.CA Cancer J Clin,2023,73(1):17-48.
［2］ 国家卫生健康委办公厅.原发性肺癌诊疗指南(2022年版)［J］.协和医学杂志,2022,13(4):549-570. General Office of National Health Commission of the People's Republic of China.Clinical practice guideline for primary lung cancer(2022 version)［J］.Medical Journal of Peking Union Medical College Hospital,2022,13(4):549-570.
［3］ LIU Y,AO X,YU W,et al.Biogenesis,functions,and clinical implications of circular RNAs in non-small cell lung cancer［J］.Mol Ther Nucleic Acids,2022,27:50-72.
［4］ NASIM F,SABATH BF,EAPEN GA.Lung cancer［J］.Med Clin North Am,2019,103(3):463-473.
［5］ SCHABATH MB,COTE ML.Cancer progress and priorities:Lung cancer［J］.Cancer Epidemiol Biomarkers Prev,2019,28(10):1563-1579.
［6］ BI H,YIN L,FANG W,et al.Association of CEA,NSE,CYFRA 21-1,SCC-Ag,and ProGRP with clinicopathological characteristics and chemotherapeutic outcomes of lung cancer［J］.Lab Med,2023,54(4):372-379.
［7］ ZHAO ST,CHEN XX,YANG XM,et al.Application of monocyte-to-albumin ratio and neutrophil percentage-to-hemoglobin ratio on distinguishing non-small cell lung cancer patients from healthy subjects［J］.Int J Gen Med,2023,16:2175-2185.
［8］ CHANG W,ZHU J,YANG D,et al.Plasma versican and plasma exosomal versican as potential diagnostic markers for non-small cell lung cancer［J］.Respir Res,2023,24(1):140.
［9］ EDER M,FRITZ-WOLF K,KABSCH W,et al.Crystal structure of human ubiquitous mitochondrial creatine kinase［J］.Proteins,2000,39(3):216-225.
［10］ SCHLATTNER U,TOKARSKA-SCHLATTNER M,WALLIMANN T.Mitochondrial creatine kinase in human health and disease［J］.Biochim Biophys Acta,2006,1762(2):164-180.
［11］ WALLIMANN T,HEMMER W.Creatine kinase in non-muscle tissues and cells［J］.Mol Cell Biochem,1994,133-134:193-220.
［12］ KEKELIDZE T,KHAIT I,TOGLIATTI A,et al.Altered brain phosphocreatine and ATP regulation when mitochondrial creatine kinase is absent［J］.J Neurosci Res,2001,66(5):866-872.
［13］ PRATT R,VALLIS LM,LIM CW,et al.Mitochondrial creatine kinase in cancer patients［J］.Pathology,1987,19(2):162-165.
［14］ AMAMOTO R,UCHIUMI T,YAGI M,et al.The expression of ubiquitous mitochondrial creatine kinase is downregulated as prostate cancer progression［J］.J Cancer,2016,7(1):50-59.
［15］ QIAN XL,LI YQ,GU F,et al.Overexpression of ubiquitous mitochondrial creatine kinase (uMtCK) accelerates tumor growth by inhibiting apoptosis of breast cancer cells and is associated with a poor prognosis in breast cancer patients［J］.Biochem Biophys Res Commun,2012,427(1):60-66.
［16］ URANBILEG B,ENOOKU K,SOROIDA Y,et al.High ubiquitous mitochondrial creatine kinase expression in hepatocellular carcinoma denotes a poor prognosis with highly malignant potential［J］.Int J Cancer,2014,134(9):2189-2198.
［17］ LI M,LIU H,LI J,et al.Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation［J］.Respir Res,2021,22(1):190.
［18］ VAUPEL P,MULTHOFF G.Revisiting the Warburg effect:historical dogma versus current understanding［J］.J Physiol,2021,599(6):1745-1757.
［19］ PASCALE RM,CALVISI DF,SIMILE MM,et al.The Warburg effect 97 years after its discovery［J］.Cancers (Basel),2020,12(10):1-33.

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