|Table of Contents|

Study on the growth inhibition of Xihuang Pill on 4T1 murine breast cancer model and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 20
Page:
3828-3833
Research Field:
Publishing date:

Info

Title:
Study on the growth inhibition of Xihuang Pill on 4T1 murine breast cancer model and its mechanism
Author(s):
FAN Huiting; QI Xin; GUAN Nianbo; PEI Yingxia; ZHANG Yun; HOU Wei; LIN Hongsheng
Department of Oncology,Guang'anmen Hospital,China Academy of Chinese Medical Sciences,Beijing 100053,China.
Keywords:
Xihuang Pill; myeloid-derived suppressor cells; tumor immunity; cytokines
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.20.002
Abstract:
Objective:To investigate the inhibitory effect of Xihuang Pill (XHP) on the growth of 4T1 tumor-bearing mice and its immune regulatory mechanism.Methods:The murine breast cancer model was established to observe the effects of different doses of XHP on tumor growth.The proportion of myeloid-derived suppressor cells (MDSCs) in peripheral blood,spleen and bone marrow of tumor-bearing mice was detected by flow cytometry,and the proportion of ROS,CD3+T,CD8+T and CD8+T IFN-γ+ cells in spleen of mice was detected.The contents of IL-1β,IL-6 and MCP-1 in peripheral blood of tumor-bearing mice were detected by Luminex liquid chip technology.Results:XHP 0.9,0.6 and 0.3 g/kg treatment groups showed certain inhibitory effects on tumor growth (P＜0.01 or P＜0.05),among which the inhibitory effect of 0.9 g/kg group was the most obvious,and the tumor inhibitory rate reached 40%.XHP decreased the proportion of MDSCs in peripheral blood,bone marrow and spleen of tumor-bearing mice,and the level of ROS in spleen cells,increased the proportion of CD3+T cells,CD8+T cells and CD8+T IFN-γ+ cells in spleen cells,and significantly reduced the expression of IL-6 and IL-1β,which were closely related to the generation of MDSCs of tumor-bearing mice.Conclusion:XHP can inhibit the growth of 4T1 breast cancer in mice by reducing the proportion of MDSCs and enhancing the anti-tumor immune function.The reduction of IL-6 and IL-1β may be one of the mechanisms of its regulation on MDSCs,and further investigation is needed for its mechanism of action.

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Memo

Memo:
National Natural Science Foundation of China(No.81603567）；国家自然科学青年科学基金项目（编号：81603567）
Last Update: 1900-01-01