|Table of Contents|

Expression and clinical significance of TCOF1 in hepatocellular carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3712-3718
Research Field:
Publishing date:

Info

Title:
Expression and clinical significance of TCOF1 in hepatocellular carcinoma
Author(s):
FU Xiangyu1; JIANG Zhixian1; YAN Zhiqiang2; ZUO Shi3
1.Guizhou Medical University,Guizhou Guiyang 550025,China;2.Department of Gastrointestinal Surgery;3.Department of Hepatobiliary Surgery,the Affiliated Hospital of Guizhou Medical University,Guizhou Guiyang 550004,China.
Keywords:
TCOF1; hepatocellular carcinoma; prognosis; Nomograms
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.19.015
Abstract:
Objective:To investigate the differential expression,prognostic value and biological function of treacle ribosome biogenesis factor 1(TCOF1) in hepatocellular carcinoma(HCC) by bioinformatics analysis,immunohistochemistry and clinical data analysis.Methods:To analyze the expression of TCOF1 in HCC tissues and its relationship with prognosis using data from the cancer genome atlas (TCGA).Immunohistochemistry was used to detect the expression of TCOF1 in 107 HCC patients and analyze its clinical significance.Kaplan-Meier curve analysis was applied to investigate the relationship between TCOF1 expression in HCC tissues and prognosis.We perform univariate and multivariate Cox regression analysis to identify independent factors associated with prognosis in HCC patients,and construct a predictive model,and draw Nomogram to visualization prediction models.Gene set enrichment analysis(GSEA) was used to explore the biological functions of TCOF1.Results:The expression level of TCOF1 in HCC tissues was significantly higher than that in normal liver tissues(P<0.001),and high expression was associated with poor prognosis of patients (P<0.05).Univariate and multivariate Cox regression analysis revealed that high expression of Fibrinogen(Fg) (HR=1.520,95%CI=1.166~1.981,P=0.002), PATA (HR=1.048,95%CI=1.004~1.094,P=0.031), and TCOF (HR=11.126,95%CI=1.002~1.265,P=0.046) were independent risk factors for the prognosis of HCC patients.A prognostic model was constructed and a column chart was drawn to predict the 1-,3-,and 5-year OS of HCC patients.Immunohistochemical detection confirmed positive expression of TCOF1 in HCC tissues while weakly positive or negative expression in adjacent liver tissues.GSEA enrichment analysis results suggested that TCOF1 may be involved in nine signaling pathways including oocyte meiosis and the p53 signaling pathway.Conclusion:TCOF1 is highly expressed in HCC tissues and is closely related withpoor prognosis of patients,suggesting that TCOF1 may be a potential biomarker for evaluating the prognosis of patients with HCC.

References:

［1］BRAY F,LAVERSANNE M,SUNG H,et al.Global cancer statistics 2022:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries ［J］.CA Cancer J Clin,2024,74(3):229-263.
［2］SANKAR K,GONG J,OSIPOV A,et al.Recent advances in the management of hepatocellular carcinoma［J］.Clin Mol Hepatol,2024,30(1):1-15.
［3］RUMGAY H,ARNOLD M,FERLAY J,et al.Global burden of primary liver cancer in 2020 and predictions to 2040［J］.J Hepatol,2022,77(6):1598-1606.
［4］LI C,WANG H,CHEN R,et al.Outcomes and recurrence patterns following curative hepatectomy for hepatocellular carcinoma patients with different China liver cancer staging［J］.Am J Cancer Res,2022,12(2):907-921.
［5］FOWLER KJ,CHERNYAK V,RONOT M,et al.Hepatocellular carcinoma:It is time to focus on prognosis［J］.Radiology,2023,307(3):e220884.
［6］GRZANKA M,PIEKIELKO-WITKOWSKA A.The role of TCOF1 gene in health and disease:Beyond treacher collins syndrome［J］.Int J Mol Sci,2021,22(5):1-19.
［7］GU W,SUN L,WANG J,et al.The oncogenic role of treacle ribosome biogenesis factor 1(TCOF1) in human tumors:a pan-cancer analysis［J］.Aging (Albany NY),2022,14(2):943-960.
［8］HU J,LAI Y,HUANG H,et al.TCOF1 upregulation in triple-negative breast cancer promotes stemness and tumour growth and correlates with poor prognosis［J］.British Journal of Cancer,2021,126(1):57-71.
［9］NIE X,ZHAO C,ZHANG Y,et al.Nucleolar protein treacle ribosome biogenesis factor 1 maintains gastric cancer cell proliferation by regulating R-loop associated DNA replication stress［J］.J Gastroenterol Hepatol,2023,38(7):1170-1180.
［10］ZHU G,XIA H,TANG Q,et al.An epithelial-mesenchymal transition-related 5-gene signature predicting the prognosis of hepatocellular carcinoma patients［J］.Cancer Cell Int,2021,21(1):166.
［11］SHEN W,SONG Z,ZHONG X,et al.Sangerbox:A comprehensive,interaction-friendly clinical bioinformatics analysis platform［J］.iMeta,2022,1(3):e36.
［12］VOGEL A,MEYER T,SAPISOCHIN G,et al.Hepatocellular carcinoma［J］.The Lancet,2022,400(10360):1345-1362.
［13］NIE X,XIAO D,GE Y,et al.TRF2 recruits nucleolar protein TCOF1 to coordinate telomere transcription and replication［J］.Cell Death & Differentiation,2020,28(3):1062-1075.
［14］SAKAI D,TRAINOR PA.Treacher collins syndrome:Unmasking the role of Tcof1/treacle［J］.The International Journal of Biochemistry & Cell Biology,2009,41(6):1229-1232.
［15］APIZI A,WANG L,WUSIMAN L,et al.Establishment and verification of a prognostic model of liver cancer by RNA-binding proteins based on the TCGA database ［J］.Transl Cancer Res,2022,11(7):1925-1937.
［16］WU C,XIA D,WANG D,et al.TCOF1 coordinates oncogenic activation and rRNA production and promotes tumorigenesis in HCC［J］.Cancer Sci,2022,113(2):553-564.
［17］JIANG W,WANG D,YU J.Recent advances in fibrinogen research in colorectal cancer［J］.Journal of Biosciences and Medicines,2023,11(04):28-38.
［18］张雪,张革红.白蛋白、前白蛋白与纤维蛋白原的比值在肿瘤中的研究进展［J］. 现代肿瘤医学,2023,31(20):3880-3884. ZHANG X,ZHANG GH.Research progress of the ratio of albumin, prealbumin and fibrinogen in tumor［J］.Modern Oncology,2023,31(20):3880-3884.
［19］邓建忠,金建华,陆文斌，等.术前外周血纤维蛋白原水平联合血小板与淋巴细胞比值的评分对结直肠癌中预后的评估价值［J］.医学研究生学报,2020,33(03):274-279. DENG JZ,JIN JH,LU WB,et al.The predictive value of preoperative fibrinogen level in peripheral blood combined with platelet-to-lymphocyte ratio in the prognosis of colorectal cancer［J］.Journal of Medical Research & Combat Trauma Care,2020,33(03):274-279.
［20］YU M,LI X,LU Y,et al.Development and validation of a novel risk prediction model using recursive feature elimination algorithm for acute-on-chronic liver failure in chronic hepatitis B patients with severe acute exacerbation［J］.Frontiers in Medicine,2021,8(1):1-11.
［21］CALO E,GU B,BOWEN ME,et al.Tissue-selective effects of nucleolar stress and rDNA damage in developmental disorders［J］.Nature,2018,554(7690):112-117.

Memo

Memo:
National Natural Science Foundation of China（No.82260535）;国家自然科学基金地区项目(编号：82260535)
Last Update: 2024-08-30