|Table of Contents|

Effects of silencing GATA4 on ovarian granulosa cells in rats with polycystic ovary syndrome

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3665-3670
Research Field:
Publishing date:

Info

Title:
Effects of silencing GATA4 on ovarian granulosa cells in rats with polycystic ovary syndrome
Author(s):
HE Cong1; HUANG Yani1; LIU Chenchen2; YUAN Dong3; SHAO Jinkun4; KOU Lingzhou1
1.Department of Oncology;2.Cardiac Function Room;3.Department of Radiology Imaging;4.Department of Ultrasound Imaging,Zhongxiang People's Hospital/Zhongxiang Hospital of People's Hospital Affiliated to Wuhan University,Hubei Jingmen 431900,China.
Keywords:
polycystic ovary syndrome; rat model; ovarian granulosa cell; GATA-binding protein 4; proliferation; apoptosis; cysteine aspartate protease 3; phosphatidylinositol-3-kinase/serine threonine kinase signaling pathway
PACS:
R733.31
DOI:
10.3969/j.issn.1672-4992.2024.19.007
Abstract:
Objective:To explore the effects and related mechanisms of silencing GATA-binding protein 4(GATA4) on proliferation and apoptosis of ovarian granulosa cells,secretory function and inflammatory response in rats with polycystic ovary syndrome(PCOS).Methods:PCOS models were prepared.The ovarian granulosa cells in PCOS rats were cultured and divided into control group(Ctrl group),siRNA-NC group and siRNA-GATA4 group.The expression of GATA4 in ovarian granulosa cells,cells proliferation and apoptosis in the three groups were detected after transfection.The levels of hormones and inflammatory factors in cell supernatant were detected by enzyme-linked immunosorbent assay(ELISA).The expressions of activated cysteine aspartate protease 3(cleaved caspase-3),phosphatidylinositol-3-kinase(PI3K) and phosphorylated-serine threonine kinase(p-AKT) proteins were detected by Western Blotting.Results:The proliferation rate of ovarian granuleous cells,expressions of GATA4,PI3K and P-Akt in siRNA-GATA4 group were significantly lower than those in Ctrl group,while apoptosis rate,expression of cleaved caspase-3 and levels of estradiol(E2),luteinizing hormone(LH),testosterone(T),interleukin-6(IL-6),IL-1β and tumor necrosis factor-α(TNF-α) in cell supernatant were significantly higher than those in Ctrl group(P＜0.05).There was no significant difference in proliferation rate of ovarian granuleous cells,apoptosis rate,expressions of GATA4,cleaved caspase-3,PI3K and P-Akt proteins or levels of E2,LH,T,IL-6,IL-1β and TNF-α between siRNA-NC group and Ctrl group(P＞0.05).Conclusion:Silencing GATA4 can inhibit proliferation and promote apoptosis of ovarian granulosa cells,aggravate abnormal hormones secretion and inflammatory response in PCOS rats,and the mechanisms may be related to down-regulating PI3K/AKT signaling pathways.

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Last Update: 2024-08-30