|Table of Contents|

Effects of NEDD4L enhancing IGF2BP2 ubiquitination on proliferation,apoptosis,migration and invasion of colorectal cancer cells SW620

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3653-3660
Research Field:
Publishing date:

Info

Title:
Effects of NEDD4L enhancing IGF2BP2 ubiquitination on proliferation,apoptosis,migration and invasion of colorectal cancer cells SW620
Author(s):
YANG Xiulian1; WANG Mei2; DONG Hui3; MENG Fanjun1; WANG Lixin1
1.Department of Clinical Laboratory,Cardio-Cerebrovascular Hospital,General Hospital of Ningxia Medical University,Ningxia Yinchuan 750002,China;2.Department of Cardiology,Gongli Hospital of Shanghai Pudong New Area,Shainghai 200000,China;3.Institute of Medical Sciences,General Hospital of Ningxia Medical University,Ningxia Yinchuan 750004,China.
Keywords:
colorectal cancer cells SW620; colorectal cancer; ubiquitin ligase; ubiquitination; cell cycle; migration and invasion
PACS:
R735.3+5
DOI:
10.3969/j.issn.1672-4992.2024.19.005
Abstract:
Objective:To investigate the effect of NEDD4L on the migration and invasion of human metastatic colorectal cancer cell SW620 and its possible mechanism.Methods:Bioinformatics analysis was used to screen the IGF2BP2/NEDD4L ubiquitination axis and construct SW620 cell line with NEDD4L overexpression.NEDD4L mRNA expression level was detected by real-time fluorescence quantitative PCR(RT-qPCR).Cell proliferation and apoptosis were detected by CCK-8 method and flow cytometry.Cell scratch and Transwell assays were used to detect cell migration and invasion.SW620 cells were treated with CHX and MG132,and the expression of IGF2BP2 protein was detected by Western blot.The recombinant plasmids of Flag-IGF2BP2,His-NEDD4L and HA-Ub were constructed,and the effect of NEDD4L on the ubiquitination level of IGF2BP2 was detected by co-immunoprecipitation in 293T cells.Results:NEDD4L overexpression was successfully achieved in SW620 cells,and the cell proliferation ability was significantly decreased(P＜0.05),and the apoptosis ability was significantly increased(P＜0.01)，simultaneous inhibition of cell cycle progression(P＜0.05).The cell migration rate and the number of cell invasion were significantly decreased(P＜0.01).Western blot results showed that overexpression of NEDD4L significantly reduced the protein expression levels of IGF2BP2,YAP and VEGFA.Co-IP results showed that NEDD4L enhanced the binding ability of IGF2BP2 to Ub.Conclusion:NEDD4L function as a tumor suppressor in colorectal cancer cells by enhancing the ubiquitination of IGF2BP2 and reducing the protein expressions of YAP and VEGFA.

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Memo

Memo:
宁夏自然科学基金（编号：2022AAC03476）
Last Update: 2024-08-30