|Table of Contents|

LncRNA FEZF1-AS1 promotes proliferation and invasion of melanoma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 19
Page:
3631-3635
Research Field:
Publishing date:

Info

Title:
LncRNA FEZF1-AS1 promotes proliferation and invasion of melanoma cells
Author(s):
LIU Bingxue1; WANG Runqian2; XUE Linyi2; PENG Huiyong3; ZHU Dongwei2
1.Department of Clinical Laboratory,Nanjing Hospital Affiliated to Nanjing University of Traditional Chinese Medicine(The Second Hospital of Nanjing)，Nanjing Public Health Medical Center,Jiangsu Nanjing 211100,China;2.Department of Immunology,Jiangsu Key Laboratory of Laboratory Medicine,School of Medicine,Jiangsu University,Jiangsu Zhenjiang 215031,China;3.Department of Clinical Laboratory,Zhenjiang First People's Hospital,Jiangsu University,Jiangsu Zhenjiang 215031,China.
Keywords:
lncRNA FEZF1-AS1; melanoma; proliferation; invasion
PACS:
R739.5
DOI:
10.3969/j.issn.1672-4992.2024.19.001
Abstract:
Objective:To investigate the effect of lncRNA FEZF1-AS1 on the proliferation and invasion of melanoma cells.Methods:lncRNA sequencing analysis was performed in melanoma tissues and adjacent tissues,and FEZF1-AS1 was the most significantly up-regulated lncRNA in melanoma tissues.The expression of lncRNA FEZF1-AS1 in melanoma cell A375 was silenced by the transfection of a lentivirus vector.Flow cytometry and qRT-PCR were used to verify the transfection effect.The proliferation and invasion ability of A375 were detected by CCK-8 and Transwell assay.The A375 cell before and after silencing lncRNA FEZF1-AS1 were subcutaneously injected into mice,and the volume,weight and survival time of tumors were detected.Western blot was used to detect the effect of lncRNA FEZF1-AS1 on Notch1 expression in A375 cell.Results:Compared with adjacent tissues,the expression of lncRNA FEZF1-AS1 was significantly increased in melanoma tissues.The expression of lncRNA FEZF1-AS1 was significantly decreased in A375 cell by lentivirus transfection.After silencing lncRNA FEZF1-AS1,the proliferation rate and invasion ability of A375 cell were decreased.After knockdown of lncRNA FEZF1-AS1,the tumor volume and weight of melanoma model mice were reduced,and the survival time was significantly prolonged.The expression of Notch1 protein in cells was decreased after silencing lncRNA FEZF1-AS1.Conclusion:LncRNA FEZF1-AS1 can promote the proliferation and invasion of melanoma cells A375,which is related to the enhancement of Notch1 signaling pathway by lncRNA FEZF1-AS1.

References:

[1]LONG GV,SWETTER SM,MENZIES AM,et al.Cutaneous melanoma［J］.Lancet,2023,402(10400):485-502.
[2] YANG N,LU J,LU Y,et al.Primary malignant melanotic melanoma and hypomelanotic melanoma of the female urethra:case series and a review of the literature in China［J］.Melanoma Res,2019,29(1):59-64.
[3] AHMED B,QADIR MI,GHAFOOR S.Malignant melanoma:Skin cancer-diagnosis,prevention,and treatment［J］.Crit Rev Eukaryot Gene Expr,2020,30(4):291-297.
[4] NEMETH K,BAYRAKTAR R,FERRACIN M,et al.Non-coding RNAs in disease:from mechanisms to therapeutics［J］.Nat Rev Genet,2024,25(3):211-232.
[5] KATOH M,KATOH M.Precision medicine for human cancers with Notch signaling dysregulation (Review)［J］.Int J Mol Med,2020,45(2):279-297.
[6] YANG M,LU H,LIU J,et al.lncRNAfunc:a knowledge base of lncRNA function in human cancer［J］.Nucleic Acids Res,2022,50(D1):1295-1306.
[7] WANG W,MIN L,QIU X,et al.Biological function of long non-coding RNA(LncRNA) xist［J］.Front Cell Dev Biol,2021,9:645647.
[8] CHOI SW,KIM HW,NAM JW.The small peptide world in long noncoding RNAs［J］.Brief Bioinform,2019,20(5):1853-1864.
[9] ZHANG Z,LU YX,LIU F,et al.lncRNA BREA2 promotes metastasis by disrupting the WWP2-mediated ubiquitination of Notch1［J］.Proc Natl Acad Sci USA,2023,120(8):e2206694120.
[10] SCHADENDORF D,VAN AKKOOI A CJ,BERKING C,et al.Melanoma［J］.Lancet,2018,392(10151):971-984.
[11] 邢志超，张佳佳，麦威.外泌体在黑色素瘤中的研究进展［J］.现代肿瘤医学，2020，28（10）：1777-1780. XING ZC，ZHANG JJ，MAI W.Research progress of exosomes in melanoma［J］.Modern Oncology，2020，28（10）：1777-1780.
[12]任宇，刘宝瑞，邹征云，等.晚期恶性黑色素瘤的靶向及免疫治疗研究进展［J］.现代肿瘤医学，2018，26（12）：1970-1974. REN Y,LIU BR,ZOU ZY,et al.Progress of targeted therapy and immunotherapy for advanced melanoma［J］.Modern Oncology，2018，26（12）：1970-1974.
[13] SARKAR D,LEUNG EY,BAGULEY BC,et al.Epigenetic regulation in human melanoma:past and future［J］.Epigenetics,2015,10(2):103-121.
[14] LI Y,GAO Y,NIU X,et al.LncRNA BASP1-AS1 interacts with YBX1 to regulate Notch transcription and drives the malignancy of melanoma［J］.Cancer Sci,2021,112(11):4526-4542.
[15] CHEN J,YIN R,LIU X.Progress in role of FEZF1-AS1 in non-small cell lung cancer［J］.Chinese Journal of Lung Cancer,2020,23(4):294-298.
[16] HUANG S,LI C,HUANG J,et al.LncRNA FEZF1-AS1 promotes non-small lung cancer cell migration and invasion through the up-regulation of NOTCH1 by serving as a sponge of miR-34a［J］.BMC Pulm Med,2020,20(1):110-124.
[17] BIAN Z,ZHANG J,LI M,et al.LncRNA-FEZF1-AS1 promotes tumor proliferation and metastasis in colorectal cancer by regulating PKM2 signaling［J］.Clin Cancer Res,2018,24(19):4808-4819.
[18] LIANG M,LI Y,DAI T,et al.lncRNA FEZF1-AS1 regulates biological behaviors of cervical cancer by targeting miRNA-1254［J］.Food Sci Nutr,2021,9(9):4722-4737.
[19] GHARAIBEH L,ELMADANY N,ALWOSAIBAI K,et al.Notch1 in cancer therapy:Possible clinical implications and challenges［J］.Mol Pharmacol,2020,98(5):559-576.
[20]徐海伟，魏刚，孟杨，等.慢病毒介导的Notch1基因沉默抑制软骨肉瘤SW1353细胞的增殖和侵袭［J］.现代肿瘤医学，2019，27（13）：2262-2267. XU HW，WEI G，MENG Y，et al.Inhibitory effect of lentivirus mediated Notch1 gene silencing on proliferation and invasion of chondrosarcoma SW1353 cells［J］.Modern Oncology，2019，27（13）：2262-2267.
[21] SOUTH AP,PURDIE KJ,WATT SA,et al.NOTCH1 mutations occur early during cutaneous squamous cell carcinogenesis［J］.J Invest Dermatol,2014,134(10):2630-2638.
[22] WIELAND E,RODRIGUEZ-VITA J,LIEBLER SS,et al.Endothelial Notch1 activity facilitates metastasis［J］.Cancer Cell,2017,31(3):355-367.
[23] ABBY E,DENTRO SC,HALL MWJ,et al.Notch1 mutations drive clonal expansion in normal esophageal epithelium but impair tumor growth［J］.Nat Genet,2023,55(2):232-245.
[24]SALEM A,WU Y,DING Q,et al.Impact of NOTCH1 expression in primary breast adenoidcystic carcinoma［J］.J Clin Pathol,2024,35(3):299-314.

Memo

Memo:
National Natural Science Foundation of China（No.82202001）;国家自然科学基金（编号：82202001）
Last Update: 2024-08-30