|Table of Contents|

PLCD3 regulates EMT process to promote proliferation,invasion and migration of colorectal cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 18
Page:
3433-3440
Research Field:
Publishing date:

Info

Title:
PLCD3 regulates EMT process to promote proliferation,invasion and migration of colorectal cancer cells
Author(s):
YU Yantao1; GAO Shuyang1; SHAN Hai2; ZHANG Chenkai2; LIU Bin3; LI Ruiqi4; WANG Daorong1
1.Northern Jiangsu People's Hospital Affiliated to Dalian Medical University,Jiangsu Yangzhou 225001,China;2.Yangzhou University,Jiangsu Yangzhou 225001,China;3.General Surgery Institute of Yangzhou,Yangzhou University,Jiangsu Yangzhou 225001,China;4.Nanjing University,Jiangsu Nanjing 210046,China.
Keywords:
PLCD3; colorectal cancer; proliferation; invasion; migration; epithelial mesenchymal transition(EMT)
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.18.006
Abstract:
Objective:To investigate the expression of phospholipase PLCD3 in colorectal cancer tissues and cells,its impact on colorectal cancer cell proliferation,invasion,migration,and the underlying mechanisms.Methods:Immunohistochemistry was employed to analyze PLCD3 expression in colorectal tissues,and prognosis data were obtained from Kaplan-Meier Plotter databases.The expression levels of PLCD3 in human immortalized colonic epithelial cells (NCM460) and colorectal cancer cells were confirmed via RT-qPCR,and genetic tools were utilized to interfere with SW620 and SW480 cells.Proliferation,migration,and invasion were assessed using colony formation assay,CCK8 proliferation assay,Scratch assay,and Transwell assay.Changes in EMT-related proteins were detected by Western blot.Results:PLCD3 expression was significantly higher in colorectal cancer tissues compared to adjacent tissues(P＜0.05),and high PLCD3 expression was strongly correlated with poor prognostic survival(P＜0.001).Knockdown of PLCD3 inhibited SW620 cell proliferation,invasion,and migration,and the relative expression of N-cadherin,MMP2,and MMP9 was significantly reduced (all P＜0.01),and the expression level of E-cadherin was obviously increased (P＜0.001).Overexpression of PLCD3 promoted the proliferation,invasion and migration of SW480 cells,and the relative expression of N-cadherin,MMP2 and MMP9 was greatly elevated (all P＜0.001),while the expression level of E-cadherin was significantly reduced (P＜0.000 1).Conclusion:Colorectal cancer exhibits up-regulated PLCD3 expression,which may promote colorectal cancer cell proliferation,invasion,and migration by regulating the EMT process.

References:

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江苏省扬州市科技计划项目（编号：YZ2020159）
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