|Table of Contents|

Construction of a KRAS conditional mutation mouse lung adenocarcinoma model

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 18
Page:
3420-3426
Research Field:
Publishing date:

Info

Title:
Construction of a KRAS conditional mutation mouse lung adenocarcinoma model
Author(s):
CHEN Yong1; 2; WU Jun3; WANG Mengmeng1; WANG Xiaolin4; SHU Yusheng4
1.Dalian Medical University,Liaoning Dalian 116004,China;2.Department of Thoracic Surgery,Sichuan Cancer Hospital & Institute,Sichuan Cancer Center,Sichuan Chengdu 610041,China;3.Yangzhou University,Jiangsu Yangzhou 225009,China;4.Department of Thoracic Surgery,Subei People's Hospital,Jiangsu Yangzhou 225001,China.
Keywords:
lung adenocarcinoma; mouse model; KRAS; cyclization recombination enzyme
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.18.004
Abstract:
Objective:To establish a genetically engineered a mouse model of lung adenocarcinomaand investigate the development of lung adenocarcinoma at different time points,as well as differences in tumor formation between male and female mice.Methods:C57BL/6J mice with KRASLSL-G12D conditional mutations were used.After breeding and amplification,adenovirus carrying Cre recombinase (Ad-Cre) was delivered to the lung tissue of mice via endotracheal intubation to induce mutation of the KRAS gene,thereby initiating lung tumor formation.Whole lung tissues were collected at 1~8 months post-infection,embedded in paraffin,and subjected to HE staining to compare lung adenocarcinoma tumor formation at different time points and between different sexes.Results:With increasing delivery time of Ad-Cre,the proportion of lung tumors in mice increased,reaching 100% tumor formation rate at 6 months,with tumor number and size increasing over time.In mice with lung adenocarcinoma tumors,except for atypical hyperplasia observed at 1 month post-infection with Ad-Cre,invasive lung adenocarcinomas were formed after 2 months.Additionally,there was no significant difference in lung adenocarcinoma tumor formation efficiency between female and male mice.Conclusion:KRASLSL-G12D conditional mutation mice can effectively establish a lung adenocarcinoma model through endotracheal intubation delivery of Ad-Cre,with no significant effect of gender on lung adenocarcinoma tumor formation rate.

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Memo

Memo:
江苏省卫生健康委员会老年健康科研课题项目（编号：LKZ2022019）；江苏省扬州市科技局社会发展项目（编号：YZ2023084）；江苏省扬州市创新能力建设计划项目（编号：YZ2022168）
Last Update: 1900-01-01