|Table of Contents|

miR-93-5p regulates autophagy of HepG2 cells through the PI3K/AKT pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 16
Page:
2969-2974
Research Field:
Publishing date:

Info

Title:
miR-93-5p regulates autophagy of HepG2 cells through the PI3K/AKT pathway
Author(s):
ZHOU Man; CAO Ping; HOU Yilin; GAN Dingyun; LI Guangli; CHEN Wan; WU Jun
Wuhan Third Hospital(Tongren Hospital Affiliated to Wuhan University),Hubei Wuhan 430060,China.
Keywords:
type 2 diabetes; insulin resistance; miR-93-5p; phosphatidylinositol 3-kinases/protein kinase B pathway; autophagy
PACS:
R735.7
DOI:
10.3969/j.issn.1672-4992.2024.16.007
Abstract:
Objective:To investigate the effects of miR-93-5p on proliferation,autophagy,glucose consumption and phosphatidylinositol 3-kinases (PI3K)/protein kinase B (AKT) pathway of HepG2 cells.Methods:Insulin resistance cell model was induced by high glucose concentration,miR-93-5p inhibitor and NC were designed and synthesized,combined with autophagy inhibitor 3-MA.The cells were divided into Control group,IR group,IR+inhibitor NC group,IR+inhibitor group,IR+3-MA+inhibitor group.Cell proliferation activity was detected by CCK8 method,glucose consumption and glycogen synthesis were detected by kit,and the expression of autophagy genes microtubule-associated protein 1 light chain 3 (LC3)-I,LC3-II,hepatocyte growth factor (HGF) protein and PI3K/AKT pathway proteins (AKT,p-AKT) were detected by Western-Blot method.Results:Compared with the control group,the cell proliferation activity,glucose consumption,glycogen synthesis and HGF protein,LC3-II protein expression in IR group were decreased (P＜0.01),while the expressions of LC3-I protein and p-AKT/AKT value were increased (P＜0.01).Compared with IR and IR+inhibitor NC group,cell proliferation activity,glucose consumption and glycogen synthesis,HGF protein,LC3-II protein expression were increased in IR+inhibitor group (P＜0.01),LC3-I protein and p-AKT/AKT value expression were decreased in IR+inhibitor group (P＜0.01).Compared with IR+inhibitor group,3-MA reversed the effect of miR-93-5p inhibitor.Conclusion:miR-93-5p promotes autophagy of HepG2 cells through PI3K/AKT pathway,thereby inhibiting insulin resistance and alleviating body damage caused by diabetes.

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湖北省武汉市医学科研项目（编号：WX21D17）
Last Update: 1900-01-01