|Table of Contents|

The mechanism of miR-4429 regulate endoplasmic reticulum stress pathway through PIK3R1 to affect the malignant phenotype of breast cancer

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 16
Page:
2951-2958
Research Field:
Publishing date:

Info

Title:
The mechanism of miR-4429 regulate endoplasmic reticulum stress pathway through PIK3R1 to affect the malignant phenotype of breast cancer
Author(s):
ZHANG Yinghui1; SHEN Liandi1; LI Rui1; SHANG Xiaohui1; CAO Lingcong1; YAN Jun2
1.Department of Breast,Jiading District Maternal and Child Health Hospital,Shanghai 201822,China;2.Department of Oncology,Jiading District Central Hospital,Shanghai 201800,China.
Keywords:
breast cancer; miR-4429; endoplasmic reticulum stress; PIK3R1
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.16.005
Abstract:
Objective:To investigate the effect of microRNA（miRNA） miR-4429 in breast cancer cells MDA-MB-231 proliferation,migration and apoptosis,and its regulation effect on PIK3R1 and endoplasmic reticulum (ER) stress pathway.Methods:Construct miR-4429 overexpression and knockdown MDA-MB-231 cell model by liposome transfection,and detect its efficiency through qRT-PCR.MTT assay,clone formation assay,Annexin-PI staining assay and Transwell assay were used to detect the proliferation,apoptosis,and invasion of MDA-MB-231 cells,respectively.Dual-luciferase experiment and Western blot were used to verify the targeting relationship between miR-4429 and PIK3R1.qRT-PCR and Western blot were used to investigate the regulation of miR-4429 on the ER stress pathway.Results:Established miR-4429 overexpression and knockdown MDA-MB-231 cell model.The proliferation and invasion ability of MDA-MB-231 cells in the miR-4429 mimic group were lower than those in its Ctrl group,while the apoptosis ability was higher than that in its Ctrl group (P＜0.05).miR-4429 could target to PIK3R1 and suppress its protein expression,and downregulate genes related to the ER stress pathway.Conclusion:miR-4429 targets to PIK3R1 and downregulates ER stress pathway related genes expression,inhibits the proliferation and invasion of breast cancer cells and promotes apoptosis,suggesting that miR-4429 may be a potential target for the treatment of breast cancer.

References:

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上海市嘉定区科委课题(编号：JDKW-2022-0041）
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