|Table of Contents|

Regulation of miR-129-2 on migration and invasion ability of triple negative breast cancer cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 09
Page:
1594-1599
Research Field:
Publishing date:

Info

Title:
Regulation of miR-129-2 on migration and invasion ability of triple negative breast cancer cells
Author(s):
GUO Binbin; ZHAI Xiaojian; WANG Zheng; ZHANG Hao
Department of Breast Surgery,Nanyang Central Hospital,Henan Nanyang 473000,China.
Keywords:
miR-129-2; triple negative breast cancer; migration; invasion
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.09.004
Abstract:
Objective:To detect the expression level of miR-129-2 in cancer tissues of patients with triple-negative breast cancer(TNBC),analyze the relationship between the expression of miR-129-2 and clinical data of patients,and explore its influence on the migration and invasion of cancer cells.Methods:Real-time fluorescence quantitative PCR(RT-qPCR) was used to detect the expression level of miR-129-2 in TNBC cancer tissues,adjacent tissues and cell lines,and the correlation between the expression level of miR-129-2 and clinical data of patients was analyzed statistically.The effects of miR-129-2 on the migration and invasion of cancer cells were investigated by scratch assay and Transwell assay.Results:miR-129-2 expression was down-regulated in cancer tissues of 40 TNBC patients and MDA-MB-231 cells(P＜0.05,P＜0.01),and the expression level of miR-129-2 was not related to age and menopause,but was related to tumor size,stage and lymph node metastasis.The results of scratch test showed that overexpression of miR-129-2 reduced the migration distance of cells,the healing rate of scratches decreased,and the migration ability weakened(P＜0.01).Transwell invasion assay showed that overexpression of miR-129-2 could reduce the number of invasion cells and significantly inhibit the invasion ability of cancer cells(P＜0.01).Down-regulating the level of miR-129-2 enhanced the migration and invasion ability of MDA-MB-231 cells.Conclusion:The expression of miR-129-2 is low in TNBC,and the expression of miR-129-2 is related to the severity of the disease.The expression level of miR-129-2 affects the migration and invasion ability of TNBC cancer cells.

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Last Update: 2024-03-29