|Table of Contents|

The function,clinical significance and prognostic values of KRT16 in pancreatic carcinoma

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
490-496
Research Field:
Publishing date:

Info

Title:
The function,clinical significance and prognostic values of KRT16 in pancreatic carcinoma
Author(s):
SUN Weiwei; KUANG Peng; MA Dandan; LI Zhonghu; JIN Weidong; JIANG Hui
Department of General Surgery,General Hospital of Central Theater Command,Hubei Wuhan 430070,China.
Keywords:
KRT16; pancreatic carcinoma; function; clinical significance; prognosis
PACS:
R735.9
DOI:
10.3969/j.issn.1672-4992.2024.03.018
Abstract:
Objective:To research the biological functions of KRT16 in SW1990 cell line.To observe the expression of KRT16 in pancreatic carcinoma tissues.And to analyze the relationship between KRT16 expression level and clinicopathologic feature,prognosis of pancreatic carcinoma patients.Moreover,to investigate the clinical significance and prognosis value of KRT16 in pancreatic carcinoma.And to explore possible related mechanisms.Methods:EdU immunofluorescence test,Transwell assay,scratch test and flow cytometry were used to detect the proliferation,invasion,migration and apoptosis of SW1990 cells,respectively.Meanwhile,56 cases of tissue samples and clinical case data from January 2016 to December 2018 were collected in our hospital.KRT16 protein expression in pancreatic carcinoma tissues was detected by immunohistochemistry(IHC).The correlation between KRT16 expression level and clinicopathologic feature,prognosis of pancreatic carcinoma patients were analyzed.The STRING database was used to analyze the network of proteins interacting with KRT16.Gene set enrichment analysis(GSEA) was used to predict the possible signal pathways of KRT16 in pancreatic carcinoma.Results:Cell function experiment results showed that overexpression KRT16 significantly enhanced SW1990 cell proliferation,invasion,migration and inhibited its apoptosis(P＜0.001).However,interference KRT16 significantly inhibited SW1990 cell proliferation,invasion,migration and inhanced its apoptosis(P＜0.001).IHC results showed that KRT16 protein was high expression in 36 cases of pancreatic carcinoma tissues and low expression in 20 cases.Then KRT16 protein expression level and M stage were independent risk factors affecting the prognosis of pancreatic carcinoma patients(P＜0.05).Kaplan-Meier survival curves revealed that pancreatic carcinoma patients with high KRT16 levels had shorter survival(P＜0.001).STRING database showed that there was an interaction between KRT16 and KRT5 and so on.GSEA research results showed that the KRT16 mRNA high expression samples were enriched into VEGF signaling pathway,axon guidance and so on(P＜0.05).Conclusion:KRT16 promotes the proliferation,invasion and migration ability of SW1990 cell.Meanwhile,the high expression of KRT16 may be predict an unfavorable prognosis in pancreatic carcinoma patients.Moreover,it plays an important role in pancreatic carcinoma by regulating interacting proteins KRT5 and activating VEGF signaling pathways.And it may serves as a novel prognostic factor and therapeutic target of pancreatic carcinoma.

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Memo

Memo:
National Natural Science Foundation of China（No.81902501）;国家自然科学基金项目（编号：81902501）;湖北省自然科学基金项目（编号：2021CFB500）
Last Update: 2023-12-29