|Table of Contents|

Expression of MTERF3 in human brain glioma tissues and its relationship with the prognosis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
443-450
Research Field:
Publishing date:

Info

Title:
Expression of MTERF3 in human brain glioma tissues and its relationship with the prognosis
Author(s):
LIN Yaru1; 2; LIU Ru'ai1; 2; ZI Jiaji1; 2; PU Yuanqian1; 2; CHEN Ying3; DAI Liping3; YU Min4; XIONG Wei1; 2
1.College of Basic Medical Sciences,Dali University,Yunnan Dali 671000,China;2.Key Laboratory of Clinical Biochemistry in Yunnan Provincial Universities,Yunnan Dali 671000,China;3.Department of Pathology,Dali Bai Autonomous Prefecture People's Hospital,Yunnan Dali 671000,China;4.College of Life Sciences,Yunnan University,Yunnan Kunming 650091,China.
Keywords:
MTERF3; brain glioma; immunohistochemistry; TCGA dataset; prognositic significance
PACS:
R739.41
DOI:
10.3969/j.issn.1672-4992.2024.03.009
Abstract:
Objective:To investigate the expression of mitochondrial transcription termination factor 3 (MTERF3) gene in human glioma tissues,and to analyze its relationship with clinicopathological characteristics and prognosis.Methods:RT-PCR,Western blot,immunohistochemistry were performed to analyze the mRNA and protein expression levels of MTERF3 in 28 human brain glioma tissues and 10 non-cancerous brain tissues.MTERF3 mRNA expression,clinicopathological parameters and prognosis of 522 brain glioma tissues and 10 non-cancerous brain tissues were downloaded from TCGA database by R language software.The relationship between the expression of MTERF3 mRNA and clinicopathological characteristics was analyzed.Kaplan-Meier method was used to analyze the relationship between MTERF3 mRNA expression and overall survival(OS) and disease-free survival(DFS) in human brain glioma.Cox regression model was performed for the multivariate analysis of the factors affected the prognosis of brain glioma.cBioportal was used to analyze the correlation between MTERF3 gene expression level and biomarker genes expression level in brain gliomas.Results:The immunohistochemistry results showed that MTERF3 protein was located in cytoplasma,and the positive expression rate of MTERF3 protein in brain glioma tissues was 64.29%.The positive expression rate of MTERF3 protein in high grade glioma tissues(81.25%) was higher than that in low grade glioma tissues(41.67%).The expression level of MTERF3 mRNA and protein in brain glioma tissues was significantly higher than that in non-cancerous brain tissues by RT-PCR and Western blot(P<0.05).The analysis of TCGA dataset showed that the expression level of MTERF3 mRNA in glioma tissues was significantly higher than that in non-cancerous brain tissues(P<0.01),and the expression level of MTERF3 mRNA was related to pathological type, age and WHO grade(all of which were P<0.05),but not gender,incidence site and sample type.Kaplan-Meier analysis showed that the OS and DFS of patients with high MTERF3 expression were significantly lower than those with low MTERF3 expression(Log-rank P<0.01).Cox multivariate analysis showed that age and pathological type were independent influencing factors for the poor prognosis of glioma patients(all P<0.05).The mRNA expression level of MTERF3 gene was positively correlated with that of IDH1,TP53,and MKI67 genes(P＜0.01,r>0) in glioma,but negatively correlated with that of PTEN,RBFOX3,and BRAF genes(P＜0.01,r<0).Conclusion:MTERF3 expression is significantly up-regulated in human brain glioma tissues,moreover,the expression level of MTERF3 is correlated with malignant degree and prognosis.Up-regulaion of MTERF3 could be used as a potential poor prognostic marker and therapeutic target in brain glioma.

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Memo

Memo:
National Natural Science Foundation of China（No.32160167,82160516）;国家自然科学基金项目（编号：32160167,82160516）；云南省应用基础研究专项面上项目（编号：202201AT070004,202301AT070023）；云南省昆虫生物医药研发重点实验室开放课题（编号：AG202203,AP2022006）
Last Update: 2023-12-29