|Table of Contents|

miR-223-3p affects lipopolysaccharide-induced inflammatory response in lung cancer A549 cells by modulating the TLR4/MyD88/NF-κB pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
437-442
Research Field:
Publishing date:

Info

Title:
miR-223-3p affects lipopolysaccharide-induced inflammatory response in lung cancer A549 cells by modulating the TLR4/MyD88/NF-κB pathway
Author(s):
WANG Xiansheng1; YU Hongyan1; GUO Xiaoyan1; HOU Fengyan2
1.Department of Respiratory Medicine；2.Department of Oncology，Affiliated Hospital of Hebei Engineering University，Hebei Handan 056000，China.
Keywords:
lung cancer; lipopolysaccharide; miR-223-3p; TLR4/MyD88/NF-κB pathway
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.03.008
Abstract:
Objective:To investigate the effect of miR-223-3p on lipopolysaccharide-induced inflammatory response in lung cancer A549 cells.Methods:The experiment was divided into 2 parts,each part was divided into 2 groups,total 4 groups:miRNA negative control(mimic NC)+LPS,miR-223-3p mimic(miR-223-3p mimic)+LPS and miR-223-3p mimic+BAY11-7082+LPS,miR-223-3p mimic+equal amount of solvent(vehicle)+LPS.The expression levels of TLR4/MyD88/NF-κB in each group were subsequently detected using Western blot assay,pro-inflammatory cytokine levels by enzyme-linked immunosorbent assay(ELISA),and cell viability by CCK-8 assay.Results:Compared with group mimic NC+LPS,group miR-223-3p mimic+LPS showed low TLR4/MyD88/NF-κB protein expression levels,low secretion of pro-inflammatory cytokines as well as high cell viability.Compared with group miR-223-3p mimic+BAY11-7082+LPS,group miR-223-3p mimic+vehicle+LPS showed low TLR4/MyD88/NF-κB protein expression,low secretion of pro-inflammatory cytokines as well as high cell viability.Conclusion:miR-223-3p can inhibit inflammation in lung cancer by inhibiting TLR4/MyD88/NF-κB to promote A549 cell proliferation and inhibit secretion of pro-inflammatory cytokines.This study provides a new strategy for the treatment and drug development of lung cancer in the future.

References:

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Last Update: 2023-12-29