«Previous Article|Table of Contents|Next Article»

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:

2024 03

Page:

431-437

Research Field:

Publishing date:

Info

Title:

Impacts of CircFOXK2 on malignant biological behavior of ovarian cancer cells by regulating miR-330-5p/SLC1A5 axis

Author(s):

DUAN Wei; LIU Yu

Department of Gynaecology,Binzhou Medical University Hospital,Shandong Binzhou 256600,China.

Keywords:

ovarian cancer; CircFOXK2; miR-330-5p/SLC1A5 axis; proliferation; apoptosis; migration and invasion

PACS:

R737.31

DOI:

10.3969/j.issn.1672-4992.2024.03.007

Abstract:

Objective:To investigate the impacts of CircFOXK2 on the malignant biological behavior of ovarian cancer(OC) cells and its regulatory mechanism on miR-330-5p/SLC1A5 axis in this process.Methods:SKOV3 cells of human OC cell line were grouped into NC group(non transfection),si-CircFOXK2-NC group(transfection of si-CircFOXK2-NC),si-CircFOXK2 group(transfection of si-CircFOXK2),si-CircFOXK2+anti-miR-330-5p-NC group(transfection of si-CircFOXK2 and anti-miR-330-5p-NC),and si-CircFOXK2+anti-miR-330-5p group(transfection of si-CircFOXK2 and anti-miR-330-5p).The expression of CircFOXK2,miR-330-5p and SLC1A5 mRNA in cells was detected by qRT-PCR.The expression level of SLC1A5 protein in cells was detected by Western blot.The proliferation of SKOV3 cells was detected by CCK-8.The number of SKOV3 cell clones was detected by clonogenic assay.The apoptosis of SKOV3 cells was detected by flow cytometry.The migration and invasion abilities of SKOV3 cells were measured by Transwell cell test.The double luciferase reporter gene experiment and RNA pull-down were applied to verify the targeting relationship of CircFOXK2,miR-330-5p and SLC1A5.Results:Compared with NC group and si-CircFOXK2-NC group,the expression of CircFOXK2 and SLC1A5 mRNA,expression of SLC1A5 protein,cell proliferation activity,the numbers of migration and invasion of OC cells in si-CircFOXK2 group were decreased(P£¼0.05),the expression of miR-330-5p and the rate of apoptosis were increased(P£¼0.05).The results of anti-miR-330-5p complement test showed that the expression of SLC1A5 mRNA and protein in si-CircFOXK2+anti-miR-330-5p group cells was increased,the cell proliferation activity and the ability of migration and invasion were decreased(P£¼0.05).Conclusion:Knocking-down CircFOXK2 can up-regulate the expression of miR-330-5p,down-regulate level of SLC1A5 mRNA and expression of SLC1A5 protein,and inhibit the proliferation,migration and invasion of OC cells.

References:

£Û1£ÝMU¦rOZ-GALV¦BNS,CARNERO A.Targeting cancer stem cells to overcome therapy resistance in ovarian cancer£ÛJ£Ý.Cells,2020,9(6):1402-1425.
£Û2£ÝZHANG R,SIU MKY,NGAN HYS,et al.Molecular biomarkers for the early detection of ovarian cancer£ÛJ£Ý.Int J Mol Sci,2022,23(19):12041-12057.
£Û3£ÝTIAN W,LEI N,ZHOU J,et al.Extracellular vesicles in ovarian cancer chemoresistance,metastasis,and immune evasion£ÛJ£Ý.Cell Death Dis,2022,13(1):64-76.
£Û4£ÝWEI X,LYU H,YANG S,et al.CircRNA PLOD2 enhances ovarian cancer propagation by controlling miR-378£ÛJ£Ý.Saudi J Biol Sci,2021,28(11):6260-6265.
£Û5£ÝZHENG J,YAN X,LU T,et al.CircFOXK2 promotes hepatocellular carcinoma progression and leads to a poor clinical prognosis via regulating the Warburg effect£ÛJ£Ý.J Exp Clin Cancer Res,2023,42(1):63-84.
£Û6£ÝZHANG W,LIU H,JIANG J,et al.CircRNA circFOXK2 facilitates oncogenesis in breast cancer via IGF2BP3/miR-370 axis£ÛJ£Ý.Aging(Albany NY),2021,13(14):18978-18992.
£Û7£ÝASL ER,SARABANDI S,SHADEMAN B,et al.MicroRNA targeting:A novel therapeutic intervention for ovarian cancer£ÛJ£Ý.Biochem Biophys Rep,2023,35:101519.
£Û8£ÝFU X,ZHANG L,DAN L,et al.LncRNA EWSAT1 promotes ovarian cancer progression through targeting miR-330-5p expression£ÛJ£Ý.Am J Transl Res,2017,9(9):4094-4103.
£Û9£ÝMA H,QU S,ZHAI Y,et al.circ_0025033 promotes ovarian cancer development via regulating the hsa_miR-370-3p/SLC1A5 axis£ÛJ£Ý.Cell Mol Biol Lett,2022,27(1):94-108.
£Û10£ÝWANG K,GUAN C,SHANG X,et al.A bioinformatic analysis:The overexpression and clinical significance of FCGBP in ovarian cancer£ÛJ£Ý.Aging(Albany NY),2021,13(5):7416-7429.
£Û11£ÝCHANDRA A,PIUS C,NABEEL M,et al.Ovarian cancer:Current status and strategies for improving therapeutic outcomes£ÛJ£Ý.Cancer Med,2019,8(16):7018-7031.
£Û12£ÝGENG Y,WANG M,WU Z,et al.Research progress of circRNA in malignant tumour metabolic reprogramming£ÛJ£Ý.RNA Biol,2023,20(1):641-651.
£Û13£ÝXU Q,DENG B,LI M,et al.circRNA-UBAP2 promotes the proliferation and inhibits apoptosis of ovarian cancer though miR-382-5p/PRPF8 axis£ÛJ£Ý.J Ovarian Res,2020,13(1):81-91.
£Û14£ÝZHANG Y,WANG Y,ZHAO G,et al.FOXK2 promotes ovarian cancer stemness by regulating the unfolded protein response pathway£ÛJ£Ý.J Clin Invest,2022,132(10):1-16.
£Û15£ÝZHANG N,FAN J,DENG Z.CircFOXK2 enhances tumorigenesis and immune evasion in non-small cell lung cancer by miR-485-5p/PD-L1 axis£ÛJ£Ý.Anticancer Drugs,2022,33(5):437-447.
£Û16£ÝVERDUCI L,STRANO S,YARDEN Y,et al.The circRNA-microRNA code:Emerging implications for cancer diagnosis and treatment£ÛJ£Ý.Mol Oncol,2019,13(4):669-680.
£Û17£ÝMENON A,ABD-AZIZ N,KHALID K,et al.miRNA:A promising therapeutic target in cancer£ÛJ£Ý.Int J Mol Sci,2022,23(19):11502-11531.
£Û18£ÝQIN L,SUN X,ZHOU F,et al.CircLRP6 contributes to prostate cancer growth and metastasis by binding to miR-330-5p to up-regulate NRBP1£ÛJ£Ý.World J Surg Oncol,2021,19(1):184-198.
£Û19£ÝXU S,LEI SL,LIU KJ,et al.circSFMBT1 promotes pancreatic cancer growth and metastasis via targeting miR-330-5p/PAK1 axis£ÛJ£Ý.Cancer Gene Ther,2021,28(3-4):234-249.
£Û20£ÝHAN L,ZHOU J,LI L,et al.SLC1A5 enhances malignant phenotypes through modulating ferroptosis status and immune microenvironment in glioma£ÛJ£Ý.Cell Death Dis,2022,13(12):1071-1085.
£Û21£ÝLUO H,PENG J,YUAN Y.CircRNA OXCT1 promotes the malignant progression and glutamine metabolism of non-small cell lung cancer by absorbing miR-516b-5p and upregulating SLC1A5£ÛJ£Ý.Cell Cycle,2023,22(10):1182-1195.

Memo

Memo:

-

Common functions

Last Update: 2023-12-29