|Table of Contents|

Long non-coding RNA ZMIZ1-AS1 promotes osteosarcoma progression by stabilization of ZMIZ1

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
412-419
Research Field:
Publishing date:

Info

Title:
Long non-coding RNA ZMIZ1-AS1 promotes osteosarcoma progression by stabilization of ZMIZ1
Author(s):
ZHOU YichiLI QingboCAI LeiZHOU ChuankunWANG ZhengkunKOU BowenLIU Weijun
Wuhan Fourth Hospital,Hubei Wuhan 430000,China.
Keywords:
long non-coding RNAZMIZ1-AS1osteosarcoma
PACS:
R738.1
DOI:
10.3969/j.issn.1672-4992.2024.03.004
Abstract:
Objective:To explore the role of ZMIZ1-AS1 and its downstream signaling pathway in the progression of osteosarcoma(OS).Methods:Cell proliferation,invasion,and migration were detected by colony formation,Transwell,and wound healing assays.The binding of SOX2 or MYC protein with ZMIZ1-AS1 promoter was explored by ChIP assay and dual-luciferase reporter assay.Interaction between PTBP1 protein and ZMIZ1-AS1(or ZMIZ1 mRNA) was detected by RIP assay.Results:SOX2 and MYC are the downstream effectors of the Hippo pathway and transcriptionally activated ZMIZ1-AS1.Compared with the controls,OS tissues and cells contained higher ZMIZ1-AS1 expression.Silencing of ZMIZ1-AS1 repressed OS cell viability,proliferation,migration,and invasion.Our findings further showed that ZMIZ1-AS1 recruits RNA-binding protein PTBP1 to stabilize ZMIZ1 mRNA.PTBP1 or ZMIZ1 overexpression rescues the suppressive effects of silenced ZMIZ1-AS1 on OS cellular processes.Importantly,ZMIZ1-AS1 promotes OS growth in vivo by stabilization of ZMIZ1.Conclusion:Long non-coding RNA ZMIZ1-AS1 promotes OS progression by stabilization of ZMIZ1.

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Memo

Memo:
职业病危害识别与控制湖北省重点实验室开放基金项目(编号:OHIC2020Z12);湖北省武汉市卫健委医学科研面上项目(编号:WX21D83)
Last Update: 2023-12-29