|Table of Contents|

Role of calcium binding protein S100A11 promoting the progression of colorectal cancer and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
405-411
Research Field:
Publishing date:

Info

Title:
Role of calcium binding protein S100A11 promoting the progression of colorectal cancer and its mechanism
Author(s):
NING Junya; LIU Min; ZHANG Jiaojiao; GAO Lijuan; CAO Jimin
Key Laboratory of Cell Physiology at Shanxi Medical University,Ministry of Education,Key Laboratory of Cell Physiology of Shanxi Province,and the Department of Physiology,Shanxi Medical University,Shanxi Taiyuan 030001,China.
Keywords:
S100A11; colorectal cancer; cell migration; bioinformatics; gene expression regulation
PACS:
R735.3
DOI:
10.3969/j.issn.1672-4992.2024.03.003
Abstract:
Objective:To investigate the effect and mechanism of calcium binding protein S100A11 in regulating colorectal cancer(CRC) progression.Methods:In this study,GEPIA,UALCAN and HPA databases were used to analyze the expression of S100A11 in CRC and its relationship with the clinical stage and prognosis of CRC.In addition,CRC cell lines/immortalized intestinal epithelial cell were used for verification.The effect of S100A11 on CRC cell migration and CRC biological pathway was further analyzed by RNA interference assay.Results:Database analysis showed that the expression of S100A11 in CRC tissues was significantly higher than that in normal colorectal tissues.And S100A11 was closely associated with the late stage and poor prognosis of CRC.RT-qPCR and Western blot results also showed that the expression of S100A11 was increased in CRC cells.Knocking down S100A11 in SW480 cells effectively inhibited cell migration and epithelial-mesenchymal transition(EMT) process.After silencing S100A11 expression in CRC cells,a total of 149 differentially expressed genes were screened by transcriptome sequencing and biogenic analysis.The functional networks enrichment results of these differentially expressed genes were mainly involved in inflammatory bowel disease,focal adhesion and PI3K-Akt signaling pathway.Conclusion:This study found that high expression of S100A11 is an essential factor in CRC metastasis,and preliminarily revealed the key signaling pathways regulated by S100A11 in CRC cells,providing experimental basis for further exploration of the molecular mechanism by which S100A11 regulating CRC progression.

References:

［1］SUNG H,FERLAY J,SIEGEL RL,et al.Global cancer statistics 2020:GLOBOCAN estimates of incidence and mortality worldwide for 36 cancers in 185 countries［J］.CA Cancer J Clin,2021,71(3):209-249.
［2］MORGAN E,ARNOLD M,GINI A,et al.Global burden of colorectal cancer in 2020 and 2040:Incidence and mortality estimates from GLOBOCAN［J］.Gut,2023,72(2):338-344.
［3］FENG RM,ZONG YN,CAO SM,et al.Current cancer situation in China:Good or bad news from the 2018 global cancer statistics［J］.Cancer Commun(Lond),2019,39(1):22.
［4］BURNETT-HARTMAN AN,LEE JK,DEMB J,et al.An update on the epidemiology,molecular characterization,diagnosis,and screening strategies for early-onset colorectal cancer［J］.Gastroenterology,2021,160(4):1041-1049.
［5］SONG D,ZHANG Z,LI W,et al.Judgment of benign and early malignant colorectal tumors from ultrasound images with deep multi-view fusion［J］.Comput Methods Programs Biomed,2022,215:106634.
［6］PETEROVA E,BURES J,MORAVKOVA P,et al.Tissue mRNA for S100A4,S100A6,S100A8,S100A9,S100A11 and S100P proteins in colorectal neoplasia:A pilot study［J］.Molecules,2021,26(2):402.
［7］LIANG Q,FU J,WANG X,et al.circS100A11 enhances M2a macrophage activation and lung inflammation in children with asthma［J］.Allergy,2023,78(6):1459-1472.
［8］TENG Y,XIE R,XU J,et al.Tubulointerstitial nephritis antigen-like 1 is a novel matricellular protein that promotes gastric bacterial colonization and gastritis in the setting of Helicobacter pylori infection［J］.Cell Mol Immunol,2023,20(8):924-940.
［9］ZHANG M,ZHENG S,JING C,et al.S100A11 promotes TGF-beta1-induced epithelial-mesenchymal transition through SMAD2/3 signaling pathway in intrahepatic cholangiocarcinoma［J］.Future Oncol,2018,14(9):837-847.
［10］ZHANG MX,GAN W,JING CY,et al.S100A11 promotes cell proliferation via p38/MAPK signaling pathway in intrahepatic cholangiocarcinoma［J］.Mol Carcinog,2019,58(1):19-30.
［11］GOU D,LIU R,SHAN X,et al.Gluconeogenic enzyme PCK1 supports S-adenosylmethionine biosynthesis and promotes H3K9me3 modification to suppress hepatocellular carcinoma progression［J］.J Clin Invest,2023,133(13):161713.
［12］ZENG B,CHEN Y,CHEN H,et al.Synergistic inhibition of NUDT21 by secretory S100A11 and exosomal miR-487a-5p promotes melanoma oligo-to poly-metastatic progression［J］.Mol Oncol,2023，17(12):2743-2766.
［13］GUO AJ,WANG FJ,JI Q,et al.Proteome analyses reveal S100A11,S100P,and RBM25 are tumor biomarkers in colorectal cancer［J］.Proteomics Clin Appl,2021,15(1):e2000056.
［14］TANG Z,KANG B,LI C,et al.GEPIA2:An enhanced web server for large-scale expression profiling and interactive analysis［J］.Nucleic Acids Res,2019,47(W1):W556-W560.
［15］CHANDRASHEKAR DS,KARTHIKEYAN SK,KORLA PK,et al.UALCAN:An update to the integrated cancer data analysis platform［J］.Neoplasia,2022,25:18-27.
［16］LANCZKY A,GYORFFY B.Web-based survival analysis tool tailored for medical research(KMplot):Development and implementation［J］.J Med Internet Res,2021,23(7):e27633.
［17］ZHANG H,CHEN Z,ZHANG A,et al.The role of calcium signaling in melanoma［J］.Int J Mol Sci,2022,23(3):1010.
［18］WU YY,LI XF,WU S,et al.Role of the S100 protein family in rheumatoid arthritis［J］.Arthritis Res Ther,2022,24(1):35.
［19］NIU Y,SHAO Z,WANG H,et al.LASP1-S100A11 axis promotes colorectal cancer aggressiveness by modulating TGFbeta/Smad signaling［J］.Sci Rep,2016,6:26112.
［20］MA G,DAI W,ZHANG J,et al.ELK1-mediated upregulation of lncRNA LBX2-AS1 facilitates cell proliferation and invasion via regulating miR-491-5p/S100A11 axis in colorectal cancer［J］.Int J Mol Med,2021,48(1):138.
［21］CHAUDHARY PK,KIM S.An insight into GPCR and G-proteins as cancer drivers［J］.Cells,2021,10(12):3288.
［22］ZECCHIN D,MOORE C,MICHAILIDIS F,et al.Combined targeting of G protein-coupled receptor and EGF receptor signaling overcomes resistance to PI3K pathway inhibitors in PTEN-null triple negative breast cancer［J］.EMBO Mol Med,2020,12(8):e11987.
［23］BURRIDGE K.Focal adhesions:A personal perspective on a half century of progress［J］.FEBS J,2017,284(20):3355-3361.
［24］DESCHOUT H,PLATZMAN I,SAGE D,et al.Investigating focal adhesion substructures by localization microscopy［J］.Biophys J,2017,113(11):2508-2518.
［25］JBARA A,LIN KT,STOSSEL C,et al.RBFOX2 modulates a metastatic signature of alternative splicing in pancreatic cancer［J］.Nature,2023,617(7959):147-153.
［26］SHEN J,CAO B,WANG Y,et al.Hippo component YAP promotes focal adhesion and tumour aggressiveness via transcriptionally activating THBS1/FAK signalling in breast cancer［J］.J Exp Clin Cancer Res,2018,37(1):175.
［27］KAO LY,CHAO WT.Rab11-mediated focal adhesion turnover in sarcoma cell migration［J］.Chin J Physiol,2021,64(1):43-50.
［28］PAPE J,MAGDELDIN T,STAMATI K,et al.Cancer-associated fibroblasts mediate cancer progression and remodel the tumouroid stroma［J］.Br J Cancer,2020,123(7):1178-1190.
［29］DUAN S,HUANG W,LIU X,et al.IMPDH2 promotes colorectal cancer progression through activation of the PI3K/AKT/mTOR and PI3K/AKT/FOXO1 signaling pathways［J］.J Exp Clin Cancer Res,2018,37(1):304.

Memo

Memo:
National Natural Science Foundation of China（No.82203412）;国家自然科学基金项目（编号：82203412）
Last Update: 2023-12-29