|Table of Contents|

Exosomes of breast cancer adriamycin-resistant MCF-7/ADR cells transfer tTG to participate in drug resistance of MCF-7 cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 03
Page:
393-397
Research Field:
Publishing date:

Info

Title:
Exosomes of breast cancer adriamycin-resistant MCF-7/ADR cells transfer tTG to participate in drug resistance of MCF-7 cells
Author(s):
ZHANG Huanhai1; SHEN Yuanyuan1; WANG Xiaohong2; ZHANG Xiangsheng1; CHENG Kai1
1.Yantai Affiliated Hospital of Binzhou Medical University,Shandong Yantai 264100,China;2.Binzhou Medical University Hospital,Shandong Binzhou 256600,China.
Keywords:
exosomes; tTG; breast cancer; drug resistance of adriamycin
PACS:
R737.9
DOI:
10.3969/j.issn.1672-4992.2024.03.001
Abstract:
Objective:To study the process of breast cancer adriamycin-resistant cell MCF-7/ADR participating in the drug resistance of sensitive cell MCF-7 through tTG transmitted by exosomes.Methods：This experiment first cultured MCF-7 and MCF-7/ADR cells,and then measured the content of exosomes in MCF-7 and MCF-7/ADR cells,and extracted the exosomes for later use.MCF-7/ADR exosomes(EXO/ADR) were co cultured with MCF-7 cells to establish MCF-7 exosomes resistant cells(MCF-7+EXO/ADR).Western blot was used to detect the expression level of tTG protein in MCF-7,MCF-7/ADR,and MCF-7+EXO/ADR cells.Flow cytometry was used to detect the expression level of tTG protein in sensitive cell lines exosomes(EXO/S) and drug-resistant cell lines exosomes(EXO/ADR),respectively.CCK-8 method was used to detect the drug sensitivity of MCF-7,MCF-7/ADR,and MCF-7+EXO/ADR to doxorubicin.Results：Both EXO/S and EXO/ADR expressed CD63 and TSG101,but low expression of Calnexin.Flow cytometry results showed that the tTG expression level of EXO/S was (18.3±3.63)%,and that of EXO/ADR was (46.07±8.31)%.The expression level of tTG protein in EXO/ADR was significantly higher than that in EXO/S,and the difference was statistically significant（P＜0.05）.Western blot was used to detect the expression level of tTG in MCF-7,MCF-7/ADR and MCF-7+EXO/ADR groups,and the tTG expression level of MCF-7+EXO/ADR increased.The IC50 of MCF-7/ADR cells and MCF-7+EXO/ADR cells against adriamycin was （1.91±0.18）μg/mL,（82.11±3.51）μg/mL,and （6.16±1.15）μg/mL.Conclusion：MCF-7/ADR cells may secrete exosomes to transfer tTG and participate in the process of adriamycin resistance of MCF-7.

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Memo

Memo:
山东省医药卫生科技发展计划项目（编号：202104010967）；滨州医学院“临床+X”项目（编号：滨医行发〔2022〕22号）
Last Update: 2023-12-29