|Table of Contents|

Expression of circular RNA circ_104939 in lung adenocarcinoma tissues and its clinical significance

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 01
Page:
35-41
Research Field:
Publishing date:

Info

Title:
Expression of circular RNA circ_104939 in lung adenocarcinoma tissues and its clinical significance
Author(s):
DAI Wanhui1; PENG Huiyong2; DAI Jindong3; ZHU Dongwei3
1.Department of Clinic Laboratory,Nanjing Second Hospital,Nanjing University of Chinese Medicine,Jiangsu Nanjing 210000,China;2.Department of Clinic Laboratory,the First People's Hospital of Zhenjiang,Jiangsu Zhenjiang 212000,China;3.Department of Immunology,Jiangsu Key Laboratory of Laboratory Medicine,School of Medicine,Jiangsu University,Jiangsu Zhenjiang 212000,China.
Keywords:
lung adenocarcinoma; circular RNA; circ-104939
PACS:
R734.2
DOI:
10.3969/j.issn.1672-4992.2024.01.006
Abstract:
Objective:To investigate the expression level and clinical value of circ_104939 in lung adenocarcinoma.Methods:Ten pairs of lung adenocarcinoma tumor tissues and adjacent tissues were collected and detected by circular RNA sequencing,and circ_104939 with significantly higher expression in tumor tissues was taken as the research object.The technology of qRT-PCR was used to detect the expression of circ_104939 in 30 pairs of lung adenocarcinoma tissues and adjacent tissues to verify the sequencing results.The expression of circ-104939 in lung adenocarcinoma cell lines(HCC-78,A549,KTA-7 and PC-9) and normal bronchial epithelial cells BEAS-2B were detected and analyzed by qRT-PCR.After circ_104939 was knocked down by siRNA,proliferation,migration,invasion and cell cycle changes of lung adenocarcinoma cells(KTA-7 and PC-9) were detected by MTT,plate scratches assay,Transwell assay and flow cytometry cell cycle assay.RNA pulldown and luciferase reporting assay were used to verify the possible target of circ_104939.After circ_104939 was knocked down by siRNA,lung adenocarcinoma cells(KTA-7 and PC-9) were subcutaneously injected into nude mice to construct tumor-bearing mouse models,and the volume and weight of transplanted tumor were detected.Results:The expression level of circ_104939 in lung adenocarcinoma tissues was significantly higher than that in adjacent tissues(P＜0.01).The expression level of circ_104939 in lung adenocarcinoma cells was significantly higher than that in normal bronchial epithelial cells(P＜0.05 or P＜0.01).After circ_104939 knockdown,KTA-7 and PC-9 cell proliferation,invasion ability,cell cycle progression and PC-9 cell migration ability decreased(P＜0.05 or P＜0.01).Mechanistically,circ_104939 serves as a sponge for miR-191-5p.After circ_104939 knockout,tumor volume decreased(P＜0.05 or P＜0.01),and tumor weight also decreased significantly(P＜0.01 or P＜0.001) in tumor-bearing mice.Conclusion:The expression of circ_104939 is highly increased in lung adenocarcinoma tissues,which is expected to be a promising target for diagnosis and treatment of lung adenocarcinoma.

References:

[1]ADAMS SJ,STONE E,BALDWIN DR,et al.Lung cancer screening［J］.Lancet,2023,401(10374):390-408.
[2] SUCCONY L,RASSL DM,BARKER AP,et al.Adenocarcinoma spectrum lesions of the lung:detection,pathology and treatment strategies［J］.Cancer Treat Rev,2021,99:102237.
[3] SALMENA L,POLISENO L,TAY Y,et al.A ceRNA hypothesis:the rosetta stone of a hidden RNA language［J］.Cell,2011,146(3):353-358.
[4] CHEN LL,YANG L.Regulation of circRNA biogenesis［J］.RNA Biol,2015,12(4):381-388.
[5] LI X,YANG L,CHEN LL.The biogenesis,functions,and challenges of circular RNAs［J］.Mol Cell,2018,71(3):428-442.
[6] CHEN L,SHAN G.CircRNA in cancer:fundamental mechanism and clinical potential［J］.Cancer Lett,2021,505:49-57.
[7] KRISTENSEN LS,HANSEN TB,VENO MT,et al.Circular RNAs in cancer:opportunities and challenges in the field［J］.Oncogene,2018,37(5):555-565.
[8] KRISTENSEN LS,ANDERSEN MS,STAGSTED LVW,et al.The biogenesis,biology and characterization of circular RNAs［J］.Nat Rev Genet,2019,20(11):675-691.
[9] HANSEN TB,JENSEN TI,CLAUSEN BH,et al.Natural RNA circles function as efficient microRNA sponges［J］.Nature,2013,495(7441):384-388.
[10] SHEN Y,ZHANG N,CHAI J,et al.CircPDIA4 induces gastric cancer progression by promoting ERK1/2 activation and enhancing biogenesis of oncogenic circRNAs［J］.Cancer Res,2023,83(4):538-552.
[11] KULCHESK FR,CHRISTOFF AP,MARGIS R.Circular RNAs are miRNA sponges and can be used as a new class of biomarker［J］.J Biotechnol,2016,238:42-51.
[12] SONG R,MA S,XU J,et al.A novel polypeptide encoded by the circular RNA ZKSCAN1 suppresses HCC via degradation of mTOR［J］.Mol Cancer,2023,22(1):16.
[13] ZHAO S,LI S,LIU W,et al.Circular RNA signature in lung adenocarcinoma:amioncocirc database-based study and literature review［J］.Front Oncol,2020,10:523342.
[14] ZHAO Y,ZHANG C,TANG H,et al.Mechanism of RNA circHIPK3 involved in resistance of lung cancer cells to gefitinib［J］.Biomed Res Int,2022,2022:4541918.
[15] CHEN X,MAO R,SU W,et al.Circular RNA circHIPK3 modulates autophagy via MIR124-3p-STAT3-PRKAA/AMPKα signaling in STK11 mutant lung cancer［J］.Autophagy,2020,16(4):659-671.
[16] SIEGEL RL,MILLER KD,JEMAL A.Cancer statistics,2019［J］.CA Cancer J Clin,2019,69(1):7-34.
[17] ZHANG ZH,WANG Y,ZHANG Y,et al.The function and mechanisms of action of circular RNAs in urologic cancer［J］.Mol Cancer,2023,22(1):61.
[18] SU L,ZHAO J,SU H,et al.CircRNAs in lung adenocarcinoma:diagnosis and therapy［J］.Curr Gene Ther,2022,22(1):15-22.
[19] ZHANG X,XU Y,MA L,et al.Essential roles of exosome and circRNA_101093 on ferroptosis desensitization in lung adenocarcinoma［J］.Cancer Commun(Lond),2022,42(4):287-313.
[20] YANG C,YUAN W,YANG X,et al.Circular RNA circ-ITCH inhibits bladder cancer progression by sponging miR-17/miR-224 and regulating p21,PTEN expression［J］.Mol Cancer,2018,17(1):19.
[21] LI Y,ZHENG Q,BAO C,et al.Circular RNA is enriched and stable in exosomes:a promising biomarker for cancer diagnosis［J］.Cell Res,2015,25(8):981-984.
[22] ZHOU B,MO Z,LAI G,et al.Targeting tumor exosomal circular RNA cSERPINE2 suppresses breast cancer progression by modulating MALT1-NF-［J］.J Exp Clin Cancer Res,2023,42(1):48.
[23]江丽华，王朝霞.环状RNA在肿瘤中的研究进展［J］.现代肿瘤医学，2019,27（19）：3516-3520. JIANG LH,WANG ZX.Progression of circular RNA in tumors［J］.Modern Oncology，2019,27（19）：3516-3520.
[24] LOUIS C,LECLERC D,COULOUARN C.Emerging roles of circular RNAs in liver cancer［J］.JHEP Rep,2022,4(2):100413.
[25] ZHOU LY,ZHANG FW,TONG J,et al.MiR-191-5p inhibits lung adenocarcinoma by repressing SATB1 to inhibit Wnt pathway［J］.Mol Genet Genomic Med,2020,8(1):e1043.

Memo

Memo:
National Natural Science Foundation of China（No.82202001）;国家自然科学基金（编号：82202001）；江苏大学创新创业训练计划项目（编号：202310299413X）
Last Update: 2023-11-30