|Table of Contents|

Study on the mechanism of lncRNA LUCAT1 promoting the growth and metastasis of renal clear cell carcinoma by regulating the miR-141-3p/SOX11 axis

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 01
Page:
25-34
Research Field:
Publishing date:

Info

Title:
Study on the mechanism of lncRNA LUCAT1 promoting the growth and metastasis of renal clear cell carcinoma by regulating the miR-141-3p/SOX11 axis
Author(s):
MENG Lidan1; WANG Xiaoling1; SONG Junyu2
1.Department of Nephrology,Hengshui People's Hospital,Hebei Hengshui 053000,China;2.Department of Spleen and Stomach,Hengshui Hospital of Traditional Chinese Medicine,Hebei Hengshui 053000,China.
Keywords:
LUCAT1; miR-141-3p; SOX11; kidney renal clear cell carcinoma; metastasis
PACS:
R737.11
DOI:
10.3969/j.issn.1672-4992.2024.01.005
Abstract:
Objective:To investigate the effects of lncRNA LUCAT1 on the growth and metastasis of kidney renal clear cell carcinoma(KIRC) by regulating miR-141-3p/SOX11 axis.Methods:LUCAT1 expression in KIRC was analyzed by bioinformatics,and the downstream miRNA/mRNA regulation axis was predicted.qRT-PCR was used to detect the expression of LUCAT1,miR-141-3p and SOX11,and Western blot was used to detect the expression of SOX11.The proliferation,migration and invasion of KIRC cells were observed by cell biological function experiment.Dual luciferase assay verified the targeting binding relationship between LUCAT1,between miR-141-3p and miR-141-3p and SOX11.The binding relationship between LUCAT1 and miR-141-3p was verified by RIP experiments.The effect of LUCAT1 on tumor growth was observed in nude mice.Results:LUCAT1 and SOX11 were highly expressed in KIRC,while miR-141-3p was low expressed in KIRC by bioinformatics and cell expressions.Results of MTT,wound healing and cell invasion experiments showed that LUCAT1 promoted the proliferation,migration and invasion of KIRC cells.Subsequently,double luciferase experiments showed that LUCAT1 could act as a molecular sponge of miR-141-3p,and we also confirmed that miR-141-3p could restore the promoting effect of LUCAT1 on the proliferation,migration and invasion of KIRC cells.Further studies showed that SOX11 was a direct target of miR-141-3p,and SOX11 could restore the inhibitory effect of miR-141-3p on the proliferation,migration and invasion of KIRC cells.Finally,we also confirmed that LUCAT1 can promote the growth of KIRC tumors in vivo through in vivo experiments.Conclusion:LUCAT1 is upregulated in KIRC,and can promote the growth and metastasis of KIRC by regulating SOX11 through adsorption of miR-141-3p.This suggests that LUCAT1 is promising as a biomarker and potential molecular therapeutic target for KIRC.

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Last Update: 2023-11-30