|Table of Contents|

The effects of lncRNA TFAP2A-AS1/miR-9-5p on proliferation,invasion and migration of endometrial carcinoma cells through ERK pathway

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 01
Page:
18-24
Research Field:
Publishing date:

Info

Title:
The effects of lncRNA TFAP2A-AS1/miR-9-5p on proliferation,invasion and migration of endometrial carcinoma cells through ERK pathway
Author(s):
ZHAO Yanhong; ZHAO Da; FU Lin; SU Dan; GUO Bo
Hainan Women and Children Medical Center,Hainan Haikou 570100,China.
Keywords:
endometrial carcinoma; lncRNA TFAP2AAS1; miR-9-5p; ERK pathway
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.01.004
Abstract:
Objective:To investigate the effect and mechanism of lncRNA TFAP2A-AS1 on the proliferation,invasion and migration of endometrial cancer cells.Methods:50 cases of endometrial carcinoma tissues and corresponding adjacent tissues were selected.Endometrial carcinoma cell lines(RL95-2,HEC-1A,HHUA,HEC-1B and Ishikawa) and endometrial epithelial cells hEEC were selected.Ishikawa transfected si-TFAP2A-AS1(si-TFAP2A-AS1 group),si-NC(si-NC group),miR-9-5p mimics(miR-9-5p group) and miR-NC(miR-NC group).RL95-2 cells were transfected with OE-TFAP2A-AS1(TFAP2A-AS1 group),Vector(Vector group),sh-miR-9-5p(sh-miR-9-5p group) and sh-NC(sh-NC group).CCK-8 was used to detect cell proliferation.Transwell was used to detect cell invasion and migration.Double luciferase and pull down assays were used to analyze the targeting relationship between TFAP2A-AS1 and miR-9-5p and the targeting relationship between miR-9-5p and ERK.The expression levels of TFAP2A-AS1 and miR-9-5p in endometrial carcinoma tissues,adjacent tissues,endometrial carcinoma cells,and hEEC cells were detected by real-time fluorescent quantitative PCR(RT-qPCR).The levels of extracellular regulated protein kinase(ERK) and phosphorylated ERK(p-ERK) were detected by Western blot.Results:The expression level of TFAP2A-AS1 in endometrial carcinoma was lower than that in adjacent tissues,while the expression level of miR-9-5p was higher than that in adjacent tissues(P＜0.05).The expression level of TFAP2A-AS1 and miR-9-5p in endometrial carcinoma is related to the degree of differentiation,tumor diameter,TNM stage and lymph node metastasis.The expression levels of TFAP2A-AS1 and miR-9-5p in endometrial carcinoma were negatively correlated(r=－0.782,P=0.002).Compared with hEEC cells,the expression level of TFAP2A-AS1 in RL95-2,HEC-1A,HHUA,HEC-1B and Ishikawa cells decreased,and the expression level of miR-9-5p increased(P＜0.05).Compared with si-NC group,the OD value of cells in si-TFAP2A-AS1 group at 48 h and 72 h,and the number of invasive and migratory cells increased(P＜0.05).Compared with Vector group,the OD value of TFAP2A-AS1 group at 48 h and 72 h,and the number of invasive and migratory cells decreased(P＜0.05).Compared with miR-NC group,the OD value of cells in miR-9-5p group at 48 h and 72 h,and the number of invasive and migratory cells increased(P＜0.05).Compared with sh NC group,the OD value of cells in sh-miR-9-5p group at 48 h and 72 h,and the number of invasive and migratory cells decreased(P＜0.05).TFAP2A-AS1 negatively regulates the expression levels of miR-9-5p and p-ERK.miR-9-5p can target ERK protein.Conclusion:TFAP2A-AS1 overexpression can down-regulate miR-9-5p,inhibit ERK pathway,and inhibit the malignant biological behavior of endometrial cancer cells.

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Memo:
海南省临床医学中心建设项目资助(编号:琼卫医函〔2021〕75号)
Last Update: 2023-11-30