|Table of Contents|

Effect of silencing GOLM1 on radiosensitivity of cervical cancer Siha cells and its mechanism

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 01
Page:
13-17
Research Field:
Publishing date:

Info

Title:
Effect of silencing GOLM1 on radiosensitivity of cervical cancer Siha cells and its mechanism
Author(s):
JIA Chunli; LU Pengfei; ZHANG Hua
Cancer Center，the First Affiliated Hospital of Xinjiang Medical University，State Key Laboratory of Pathogenesis,Prevention and Treatment of High Incidence Diseases in Central Asia,Xinjiang Urumqi 830000,China.
Keywords:
cervical cancer; GOLM1 gene; radiation; invasion; migration; radiosensitivity
PACS:
R737.33
DOI:
10.3969/j.issn.1672-4992.2024.01.003
Abstract:
Objective:To investigate the effect of GOLM1 on radiosensitivity of human cervical cancer Siha cells and its possible mechanism.Methods:A lentivirus vector was constructed to knock down the expression of GOLM1 in cervical cancer cell Siha,and the interference efficiency was detected by qPCR.Cells were irradiated with 4 Gy X-ray and their activity was detected using MTT assay.Cervical cancer cells irradiated with radiation were divided into GOLM1-SiRNA group,NC-SiRNA group,and GOLM1-SiRNA combined with IGF1 group.Cell morphology was observed,invasion and migration abilities were observed by cell invasion and migration experiments,and cell proliferation abilities were detected by clone formation experiments.Western blot was used to detect epithelial mesenchymal transformation related proteins and PI3K/Akt pathway proteins.Results:After irradiation,the cell activity of the knockdown group with GOLM1 expression in Siha cells decreased most significantly,and the invasive and migratory abilities of the cells decreased,as well as the clone formation ability decreased.The combination of GOLM1-SiRNA and PI3K/Akt signal transduction pathway activator IGF1 increased the ability of cell invasion and migration,and enhanced the ability of clone formation.Decreasing the expression of GOLM1 increased the expression of epithelial marker protein E-cadherin,while reducing the expression of interstitial marker proteins N-cadherin and p-Akt.After adding IGF1,the expression of E-cadherin protein decreased,while the expression of N-cadherin and p-Akt protein increased.Conclusion:The decreased expression of GOLM1 increases the sensitivity of cervical cancer cells to radiation after irradiation.This effect may be achieved by mediating the PI3K/Akt signal transduction pathway to affect the invasion and migration ability of cells.

References:

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Last Update: 2023-11-30