|Table of Contents|

LINC01006 targets miR-331-3p to regulate the proliferation,apoptosis and metastasis of multiple myeloma cells

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2024 01
Page:
7-12
Research Field:
Publishing date:

Info

Title:
LINC01006 targets miR-331-3p to regulate the proliferation,apoptosis and metastasis of multiple myeloma cells
Author(s):
LI Hang; LI Qiang; ZHU Pinwei; ZHE Nana; GE Jinli; ZHOU Zhencang; LIU Zhaoyu; CHEN Dengke
Department of Hematology,The First People's Hospital of Zunyi City(The Third Affiliated Hospital of Zunyi Medical University),Guizhou Zunyi 563000,China.
Keywords:
multiple myeloma; LINC01006; miR-331-3p; proliferation; metastasis; apoptosis
PACS:
R733.3
DOI:
10.3969/j.issn.1672-4992.2024.01.002
Abstract:
Objective:To investigate the role of LINC01006 targeting miR-331-3p in the development of multiple myeloma(MM).Methods:LINC01006 and miR-331-3p expression in plasma cells from bone marrow specimens of 39 MM patients and 22 normal controls was calculated by RT-qPCR.si-LINC01006,si-NC,miR-331-3p mimics,miR-NC,si-LINC01006+anti-miR-NC and si-LINC01006+anti-miR-331-3p were transfected into MM cell U266,respectively.CCK-8,Flow cytometry and Transwell method were applied to evaluate U266 cell inhibition rate,apoptosis rate and metastasis number.The DIANA Tools website was used to predict the interaction between LINC01006 and miR-331-3p,and which was further verified by the dual luciferase reporter gene method.Results:The expression of LINC01006 was increased,while the expression of miR-331-3p was decreased in plasma cells from bone marrow specimens of MM patients.Transfection of si-LINC01006 could increase the cell inhibition rate and apoptosis rate(P＜0.05),reduce the number of cell metastases(P＜0.05),and up-regulate miR-331-3p expression(P＜0.05).Transfection of miR-331-3p mimics could increase the cell inhibition rate and apoptosis rate(P＜0.05),and reduce the number of cell metastases(P＜0.05).miR-331-3p was found to directly bind with LINC01006.Transfection of anti-miR-331-3p significantly reversed the effect of transfection of si-LINC01006 on the inhibition rate,apoptosis rate and metastasis number of MM cells.Conclusion:Silencing LINC01006 inhibits the proliferation and metastasis and induces apoptosis of MM cells by promoting miR-331-3p expression,thereby inhibiting the development of MM.

References:

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贵州省遵义市科技计划项目［编号：遵市科合HZ（2022）96号］
Last Update: 2023-11-30