|Table of Contents|

Preparation and anti-tumor activity of gambogic acid nanopreparations

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 24
Page:
4503-4507
Research Field:
Publishing date:

Info

Title:
Preparation and anti-tumor activity of gambogic acid nanopreparations
Author(s):
LI Jiaojiao; ZHAO Mengnan; MI Dandan; WANG Rujing; LI Yuke; JING Qian; ZOU Lan; XIE Jinjin; YANG Huan; DU Xin; SHI Sanjun
School of Pharmacy,Chengdu University of Traditional Chinese Medicine，Sichuan Chengdu 611137,China.
Keywords:
gambogic acid; B16F10; nano preparations; proliferate; transfer; apoptosis; stem cell
PACS:
R730.52
DOI:
10.3969/j.issn.1672-4992.2023.24.003
Abstract:
Objective:To optimize the drug load ratio of gambogic acid liposome (GA-LIP) and albumin nanoparticles (GA-BSA NP),and to study the anti-tumor activity of GA-LIP and GA-BSA NP in vitro.Methods:GA-LIP and GA-BSA NP were prepared.The particle size and PDI were analyzed by Antonpa particle size analyzer.The encapsulation efficiency was determined by high performance liquid chromatography (HPLC).The inhibitory effect of GA,GA-LIP and GA-BSA NP on the proliferation of B16F10 was detected by MTT assay,the cell migration ability was detected by cell scab assay,the apoptosis rate was detected by flow cytometry,and the inhibitory effect of B16F10 on the proliferation of tumor stem cell spheres was detected.Results:The optimized drug load ratio of GA-LIP was 1∶5,and GA-BSA NP was 1∶10.The IC50 of GA on B16F10 cells was 0.155 0 μmol/L,GA-LIP was 0.107 8 μmol/L,GA-BSA NP was 0.081 5 μmol/L.Both GA-LIP and GA-BSA NP inhibited B16F10 cell migration,promoted cell apoptosis,and inhibited tumor stem cell spheres' growth.Conclusion:Both GA-LIP and GA-BSA NP can effectively inhibit the proliferation,migration and promote apoptosis of B16F10 cells,and inhibit the growth of tumor stem cells spheres.The NP activity of BSA in the two preparations is stronger.

References:

［1］ LIU Y,CHEN Y,LIN L,et al.Gambogic acid as a candidate for cancer therapy:A review ［J］.Int J Nanomedicine,2020,15:10385-10399.
［2］ LI M,SU F,ZHU M,et al.Research progress in the field of gambogic acid and its derivatives as antineoplastic drugs ［J］.Molecules,2022,27(9):2937.
［3］ JIANG LL,LI K,LIN QH,et al.Gambogic acid causes fin developmental defect in zebrafish embryo partially via retinoic acid signaling ［J］.Reproductive Toxicology (Elmsford,N.Y.),2016,63:161-168.
［4］ LIU YT,HAO K,LIU XQ,et al.Metabolism and metabolic inhibition of gambogic acid in rat liver microsomes ［J］.Acta Pharmacologica Sinica,2006,27(9):1253-1258.
［5］ HATAMI E,JAGGI M,CHAUHAN SC,et al.Gambogic acid:A shining natural compound to nanomedicine for cancer therapeutics ［J］.Biochimica et Biophysica Acta.Reviews on Cancer,2020,1874(1):188381.
［6］ 白皎皎,陈新棉,曾代文,等.藤黄酸抗肿瘤作用机制及其纳米制剂的研究进展 ［J］.华西药学杂志,2018,33(06):546-549. BAI JJ,CHEN XM,ZENG DW,et al.Research progress on the antitumor mechanism of gambogic acid and its nano preparations ［J］.West China Journal of Pharmaceutical Sciences,2018,33(06):546-549.
［7］ 赵广超,庄婕.藤黄酸的制剂研究进展 ［J］.中国药师,2020,23(07):1404-1408. ZHAO GC,ZHUANG J,Research progress of gambogic acid preparation ［J］.China Pharmacist,2020,23(07):1404-1408.
［8］ 仲曼,胡慧慧,缪明星,等.纳米药物制剂体内分析方法及药动学研究进展和问题策略分析 ［J］.药物评价研究,2022,45(07):1413-1425. ZHONG M,HU HH,MIAO MX,et al.Research progress and problem strategy analysis of in vivo analysis methods and pharmacokinetics of nano-drug preparation ［J］.Drug Evaluation Research,2022,45(07):1413-1425.
［9］ WANG S,CHENG K,CHEN K,et al.Nanoparticle-based medicines in clinical cancer therapy ［J］.Nano Today,2022,45:101512.
［10］ SHEN X,LIU X,LI T,et al.Recent advancements in serum albumin-based nanovehicles toward potential cancer diagnosis and therapy ［J］.Frontiers in Chemistry,2021,9:746646.
［11］ 刘静,唐浩,米鹏,等抗肿瘤纳米药物临床转化研究进展 ［J］.科技导报,2018,36(22):118-126. LIU J,TANG H,MI P,et al.,Progress in clinical transformation of antitumor nanodrugs ［J］.Science & Technology Review,2018,36(22):118-126.
［12］ 武恒仪,潘娅,杨佳.藤黄酸纳米脂质体的制备与优化 ［J］.当代化工,2019,48(12):2785-2788. WU HY,PAN Y,YANG J,Preparation and optimization of gambogic acid nano-liposomes ［J］.Contemporary Chemical Industry,2019,48(12):2785-2788.
［13］ HAN L,ZHAN H,SUN X,et al.A density-changing centrifugation method for efficient separation of free drugs from drug-loaded particulate delivery systems ［J］.AAPS J,2019,21(3):33.
［14］ SHEN S,XU X,LIN S,et al.A nanotherapeutic strategy to overcome chemotherapeutic resistance of cancer stem-like cell ［J］.Nat Nanotechnol,2021,16(1):104-113.
［15］ 曹珊珊.氢溴酸常山酮微球制剂和多室脂质体制剂的研究 ［D］.上海:上海交通大学,2017:6. CAO SS.Study on microsphere preparation and multi-compartment liposome preparation of haloshanone hydrobromide ［D］.Shanghai:Shanghai Jiao Tong University,2017:6.
［16］ 赵东陆，邢琦，宋航，等.藤黄酸通过调节PPARγ表达与内质网应激抑制弥漫性大B细胞淋巴瘤生长［J］.现代肿瘤医学，2022,30（05）：773-778. ZHAO Donglu,XING Qi,SONG Hang,et al.Gambogic acid inhibits the growth of diffuse large B-cell lymphoma by regulating PPARγ expression and endoplasmic reticulum stress［J］.Modern Oncology,2022,30（05）：773-778.
［17］ 韩旭萌,焦明丽,詹合琴.藤黄酸作用靶点及相关信号通路研究进展 ［J］.新乡医学院学报,2020,37(07):696-701. HAN XM,JIAO ML,ZHAN HQ,Research progress of gambogic acid targets and related signaling pathways ［J］.Journal of Xinxiang Medical University,2020,37(07):696-701.
［18］ XU H,ZHANG D,WEI R,et al.Gambogic acid induces pyroptosis of colorectal cancer cells through the GSDME-dependent pathway and elicits an antitumor immune response ［J］.Cancers,2022,14(22):5505.
［19］ 李建雄,耿爽,胡树坚,等.脂质体递送系统功能结构设计与应用研究进展 ［J］.化工进展,2023,42(04):1-12. LI JX,GENG S,HU SJ,et al.Progress in functional structure design and application of liposome delivery system ［J］.Chemical Industry and Engineering Progress,2023,42(04):1-12.
［20］ 张曼玉,楼晨曦,曹傲能.主动靶向载药脂质体在肿瘤治疗中的研究进展 ［J］.生物医学工程学杂志,2022,39(03):633-638. ZHANG MY,LOU CX,CAO AN.Active targeting of drug-carrying liposomes in the treatment of cancer ［J］.Journal of Biomedical Engineering,2022,39(03):633-638.
［21］ ABBASI H,KOUCHAK M,MIRVEIS Z,et al.What we need to know about liposomes as drug nanocarriers:an updated review ［J］.Advanced Pharmaceutical Bulletin,2023,13(1):7-23.
［22］ SOLANKI R,ROSTAMABADI H,PATEL S,et al.Anticancer nano-delivery systems based on bovine serum albumin nanoparticles:A critical review ［J］.International Journal of Biological Macromolecules,2021,193(Pt A):528-540.
［23］ BABAEI G,AZIZ SG,JAGHI NZZ.EMT,cancer stem cells and autophagy; the three main axes of metastasis ［J］.Biomedicine Pharmacotherapy,2021,133:110909.
［24］ WALCHER L,KISTENMACHER AK,SUO H,et al.Cancer stem cells-origins and biomarkers:perspectives for targeted personalized therapies ［J］.Frontiers in Immunology,2020,11:1280.
［25］ 王雨枫,徐岷.细胞外基质与肿瘤干细胞相互作用的研究进展［J］.现代肿瘤医学，2021,29(16):2922-2926. WANG Yufeng,XU Min.Research progress on the interaction between extracellular matrix and cancer stem cells［J］.Modern Oncology,2021,29(16):2922-2926.

Memo

Memo:
National Natural Science Foundation of China(No.82003689);国家自然科学基金青年项目（编号：82003689）；四川省杰出青年科技基金（编号：2022JDJQ0052）
Last Update: 1900-01-01