|Table of Contents|

Screening of differential miRNA in serum exosomes of patients with papillary thyroid carcinoma with different iodine levels

Journal Of Modern Oncology[ISSN:1672-4992/CN:61-1415/R]

Issue:
2023 23
Page:
4351-4356
Research Field:
Publishing date:

Info

Title:
Screening of differential miRNA in serum exosomes of patients with papillary thyroid carcinoma with different iodine levels
Author(s):
LUO Xiaoting1; ZHANG Linlin2; LIU Qinghua3; ZHANG Hui4; XING Hao5; YIN Fengyan5; HE Qian1; KONG Wei6; XING Weijia1
Keywords:
papillary thyroid carcinoma; high iodine; exosome; miRNA; biomarker
PACS:
R736.1
DOI:
10.3969/j.issn.1672-4992.2023.23.010
Abstract:
Objective:To explore biomarkers that can stably diagnose papillary thyroid carcinoma(PTC) without the influence of high iodine,the expression profiles of serum exosomal miRNA in patients with PTC and healthy subjects at different iodine levels were studied.Methods:Serum samples from PTC patients and healthy people were collected,and serum iodine was determined by catalytic spectrophotometry.PTC patients with high iodine and normal iodine were 10 cases respectively,and healthy people with high iodine and normal iodine were 5 cases respectively.Then,exosome was extracted from serum.MiRNAs of exosome were extracted and performed by high-throughput sequencing.Bioinformatics analysis of target gene was done by method of gene ontology(GO) and Kyoto encyclopedia of genes and genomes(KEGG).Results:The differential-expressed miRNAs of PTC patients and healthy people in the high iodine and normal iodine groups were screened separately.They were intersected from these two groups to obtain 12 common differential-expressed miRNAs,which were significantly enriched to 6 pathways related to PTC.Conclusion:Compared with healthy people,miR-3158-3p and miR-186-5p are down-regulated in PTC patients,and miR-122-5p,miR-375-3p,miR-10a-5p,miR-10b-5p,miR-148a-3p,miR-223-5p,let-7d-3p,miR-651-5p are up-regulated.These 10 miRNAs can be used as stable potential biomarkers to distinguish PTC patients from healthy individuals in the general population.

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Last Update: 2023-10-31